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61 articles for “PCR detection”
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Research ArticleOpen accessPhrase in the titleEffects of Aerobic Exercise on PPAR-γ Expression in Adipose Tissue and RT-PCR Detection AnalysisXiaodong Zhan, Chenguang Bi, Jie WeiThis study aimed to examine the effect of organized aerobic exercise on the expression level of Peroxisome Proliferator-Activated Receptor gamma (PPAR-γ) gene in adipose tissue by performing digital variant of RT-PCR, Droplet Digital PCR (ddPCR) which is a highly sensitive method for counting the gene copy numbers. The proposed scheme consists of three-stage analytical procedure, namely, Subphase I Controlled Aerobic … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 29 Sep 2026 · 580 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease VirusAsmarani Kusumawati, Tenri A. Wanahari, Issabellina D. Tampubolon, Basofi A. MappakayaJembrana disease virus is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. Infection of Jembrana Disease Virus (JDV) on Bali cattle have caused substantial economic losses for farmers in Indonesia and Australia. In order to control the spread, development of a sensitive detection method is important. In this study, we used three … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 2 · 26 Jun 2015 · 7,798 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleRapid Real Time PCR Based Detection of Cell Count in Case of Urinary Tract InfectionPoulomi Nandy, Ashoke Ranjan Thakur, Shaon Ray ChaudhuriMicrobial identification and antimicrobial susceptibility testing methods currently used in clinical microbiology laboratories require at least two to three days because they rely on the growth and isolation of micro-organisms. This long, but necessary, delay has enormous consequences on prophylactic usage of antimicrobial drugs. This study was an attempt to reduce this detection time span. Taq Man Real Time PCR … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 2 · 22 Jun 2013 · 8,214 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEIAV Effectiveness Detection by Nested PCR Comparing two Different Samples: PBMCs and Bronchoalveolar WashElizangela Maira Dos Santos, Pedro Moreira Couto Motta, Marcos Bryan Heinemann et al.Problem statement: Equine Infectious Anemia (EIA) is a chronic, relapsing infectious disease of horses caused by Equine Infectious Anemia Virus (EIAV). The objective of this experiment was compared EIAV effectiveness detection in Peripheral Blood Mononuclear Cells (PBMCs) and Bronchoalveolar wash (BAW) from naturally infected horses. Approach: Fifty seven DNA samples isolated from PBMCs and BAW of naturally infected animals were … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 7,462 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDetection and Localization of Anti and Pro-apoptotic mRNA Genes in Human Colorectal Cancer Using in situ RT-PCRMohd. Nazil Salleh, Boo Siaw Shi, Wan Khairuzzaman Wan Ramli et al.Problem statement: Recent studies using conventional Polymerase Chain Reaction have shown that anti-apoptotic (Cyclooxygenase-2, COX-2 and Nuclear Factor-κB, NF-κB) and proapoptotic mRNA (Bax and Bad) are involved in the tumorigenesis of colorectal cancer. The aim of this study was to localize the expression of anti and pro-apoptotic mRNA genes using Reverse Transcription in situ Polymerase Chain Reaction (RT in situ … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,441 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEstablishment and Application of Rapid Quantitative Detection Technology for Bacillus cereusFeilong Sun, Hanyu Li, Jingxue Qian et al.Infections caused by Bacillus cereus often occur, causing a certain degree of health hazards and economic losses to people around the world. Rapid quantitative detection is of great significance for prevention. This study aims to develop a simple and rapid quantitative detection method for B. cereus, which can be used to monitor the contamination of B. cereus in food samples. … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 1 · 21 Mar 2023 · 5,564 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPolymerase Chain Reaction Detection of Pasteurella multocida Type B: 2 in Mice Infected with Contaminated River WaterFaez Firdaus Jesse Abdullah, Mohammed Muqdad Khaleel, Lawan Adamu et al.Hemorrhagic septicemia is an acute, deadly disease of cattle and buffaloes associated with colossal economic loss in the livestock industry in the Asian regions particularly Malaysia. Therefore, this study was conducted to investigate on the Polymerase chain reaction detection of Pasteurella multocida type B: 2 in mice inoculated through different routes with river water contaminated with infected mice carcasses. Sixty … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 3 · 17 Sep 2013 · 7,392 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetection of Listeria monocytogenes in Ready-to-Eat Meat ProductsAsma M.M.A. Abdelgadir, Kunwar K. Srivastava, P. Gopal ReddyProblem statement: The objective of this study was to develop a rapid and sensitive method for the detection of Listeria monocytogenes and to screen large number of ready-to-eat (RTE) meat samples. Approach: A total of 250 fresh RTE meat samples of different varieties (chicken, turkey, beef, pork and cold cuts) were purchased from local grocery stores. Results: From the 250 … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 4 · 30 Jan 2012 · 7,421 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetection of Phytoplasma on Indian Fig (Opuntia ficus-indica Mill) in Mexico Central RegionRicardo Hernández-Pérez, Juan Carlos Noa-Carrazana, Ricardo Gaspar et al.Problem statement: The Indian fig (a species of prickly pear cactus), has been known as Nopal, comprises an approximate area of 100,000 ha, in plantations used for human consumption. "Pyramids" Indian fig area located in the northeastern State of Mexico has been an important Indianfig area in the country, with 15810 ha, where a phytoplasma has been consistently present in … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,729 accessesView PDFCite
Research ArticleOpen accessIn the abstractCo-infestation of Honey Bee Colonies with Tropilaelaps mercedesae and Varroa destructor in the Republic of KazakhstanUlzhan Nuraliyeva, Gaukhar Moldakhmetova, Oleg Krupskiy et al.Beekeeping is a well-developed industry in the Republic of Kazakhstan; however, significant winter colony losses necessitate the annual import of thousands of honey bee package colonies from neighboring southern countries. The recent detection of the invasive mite Tropilaelaps mercedesae in several of these source countries poses a serious biosecurity threat to Kazakhstani apiculture. This study aimed to identify potentially infested … …American Journal of Animal and Veterinary Sciences, Vol. 21, Issue 1 · 11 Apr 2026 · 1,850 accessesView PDFCite