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8 articles for “O. F. Gorbachenko”
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Research ArticleOpen accessIn the author namesSSR Analysis of the Chloroplast and Mitochondrial Genomes of Cultivar Lines and Wild Types of the Sunflower (Helianthus L.)Vladimir Arturovich Khachumov, Alexander Vyacheslavovich Usatov, Kirill Vitalievich Azarin et al.SSR analysis of chloroplast and mitochondrial genomes of cultivar lines and wild species of sunflower (Helianthus L.) were completed. The polymorphism of 18 microsatellite loci of the chloroplast and mitochondrial genomes was investigated. Based on the obtained data was constructed dendrogram showing the genetic diversity of chloroplast and mitochondrial genomes of sunflowers. The studied species were clustered into annual and … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 19 Feb 2020 · 7,676 accessesView PDFCite
Research ArticleOpen accessIn the author namesComparative Analysis of the Complete Chloroplast Genome of the Alloplasmic Sunflower (Helianthus L.) Lines with Various CMS TypesKirill Azarin, Maxim Makarenko, Alexander Usatov et al.The complete chloroplast genomes of sunflower fertile line HA89 and isonuclear CMS lines with four different cytoplasmic backgrounds (PET1, PET2, ANN2 and MAX) were sequenced. A total of 451 polymorphic sites, with including 58 SSRs, 317 SNPs and 76 microindels were revealed between the fertile and CMS cytotypes. Among the alloplasmic male-sterile lines, cpDNA of CMS-MAX had the largest number … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 1 · 2 Mar 2018 · 9,419 accessesView PDFCite
Research ArticleOpen accessIn the author namesThe Investigation of Organelle Genomes of Extra Nuclear Sunflower Mutants with Variegated PhenotypeIrina Dremuk, Nikolay Kozel, Mariya Logacheva et al.The comparative analysis of complete chloroplast and mitochondrial DNA sequences of the original inbred line 3629 and three extra nuclear mutant lines variegated-1, variegated-15, variegated-17, obtained by the N-nitroso-N-methylurea induced mutagenesis, was done. The studied mutant lines were presented two different phenotypes with diverse chlorophyll content: Pale/green mutant line variegated-17 with 1.8% relative (to 3629 line) chlorophyll content in mutant … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 24 Oct 2017 · 7,638 accessesView PDFCite
InvestigationOpen accessIn the author namesA State of PSI and PSII Photochemistry of Sunflower Yellow-Green Plastome MutantMaksim S. Makarenko, Nikolay V. Kozel, Alexander V. Usatov et al.Chlorophyll deficient mutants are appropriate model objects for a large number of investigations in the field of photosynthesis. In this study, we identified a state of Photosystem I (PSI) and Photosystem II (PSII) photochemistry and light responses of the sunflower yellow-green mutant line - en:chlorina-7, wherein mutations in chloroplast genes have been previously localized. The conducted research revealed low content … …OnLine Journal of Biological Sciences, Vol. 16, Issue 4 · 29 Dec 2016 · 7,462 accessesView PDFCite
Research ArticleOpen accessIn the author namesComparative Genomics of Domesticated and Wild Sunflower: Complete Chloroplast and Mitochondrial GenomesM. S. Makarenko, A. V. Usatov, N. V. Markin et al.The entire chloroplast and mitochondrial genomes of domesticated and wild type sunflower were sequenced. The comparative analysis of chloroplast genomes revealed 43 variant sites, including 21 polymorphic SSR loci and 22 SNPs. About 14 variant sites were found by collation of mitochondrial DNA (mtDNA), among them 4 SSRs, 8 SNPs and 2 deletions. About 9 SNPs were located in coding … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 29 Feb 2016 · 7,778 accessesView PDFCite
Research ArticleOpen accessIn the author namesSSR Analysis of Maternal and Paternal Lines Selected in the Don Region (Russia)N. V. Markin, A. V. Usatov, V. N. Vasilenko et al.Evaluation of DNA polymorphisms of breeding material of sunflower from L.A. Zhdanov Don Experimental Station of oil Crops of V.S. Pustovoit All-Russian Research Institute of Oil Crops represented by 17 maternal (CMS) lines and 12 paternal (Rf) lines was conducted. There were identified 35 allelic variants of CMS lines and 42 allelic variants of Rf lines with use of 11 … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 1 · 16 Jan 2016 · 7,556 accessesView PDFCite
Research ArticleOpen accessIn the author namesDNA-MARKERS OF SUNFLOWER RESISTANCE TO THE DOWNY MILDEW (PLASMOPARA HALSTEDII)A. V. Usatov, A. I. Klimenko, K. V. Azarin et al.One of the areas of biotechnology sunflower is the development and testing of DNA markers of important agronomic traits and in particular markers of resistance to downy mildew. Resistance of 16 Rf-lines of sunflower to the races 330 and 710 of Plasmopara halstedii has been studied. Genotyping of these lines was carried out using 9 STS-markers of three Pl-loci, Pl5, … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 2 · 24 Jul 2014 · 7,427 accessesView PDFCite
Research ArticleOpen accessIn the author namesTHE RELATIONSHIP BETWEEN HETEROSIS AND GENETIC DISTANCES BASED ON SSR MARKERS IN HELIANTHUS ANNUUSA. V. Usatov, A. I. Klimenko, K. V. Azarin et al.Identifying the best inbred combinations for the development of commercial hybrid of sunflower remains the main challenge to sunflower breeders. In the present research the level of heterosis of F1 hybrids, genetic diversity of parental lines based on SSR markers, as well as its connection with specific combining ability of sunflower were studied. Ten sunflower elite inbred lines (3 restorer … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 7 Mar 2014 · 8,007 accessesView PDFCite