5 articles for “NifH”

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Research ArticleOpen accessEvery word in the titleDiscovery of Evolutionary Divergence of Biological Nitrogen Fixation and Photosynthesis: Fine Tuning of Biogenesis of the NifH and the ChlL by a Peptidyl-Prolyl Cis/Trans IsomeraseNara Gavini, Sinny Delacroix, Kelvin Harris Jr., Lakshmi PulakatProblem statement: Despite the structural and functional similarities between the nitrogenase that performs biological nitrogen fixation reaction and the Dark Protochlorphyllide Oxidoreductase (DPOR) that performs chlorophyll-biosynthesis, attempts to substitute nitrogenase-components with DPOR-components have hitherto failed. This investigation was undertaken to test if Chlamydomonas reinhardtii protochlorophyllide (Pchlide) reductase (ChlL) that shares some structural similarity with Nitrogenase Reductase (NifH) could complement the … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 17 Feb 2012 · 7,614 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleNifH: Structural and Mechanistic Similarities with Proteins Involved in Diverse Biological ProcessesSurobhi Lahiri, Lakshmi Pulakat, Nara GaviniThe NifH protein is a subunit of the nitrogenase enzyme that catalyzes the reduction of atmospheric nitrogen to ammonia. This protein contains highly conserved regions including the nucleotide binding sites, metal center ligands and the Switch I and Switch II domains. A number of proteins have structural and mechanistic similarities as well as evolutionary relationships with the NifH protein, notable … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 6,671 accessesView PDF Cite

Research ArticleOpen accessIn the abstractThe NifX Protein is Involved in the Final Stages of FeMo-cofactor Transport to the MoFe ProteinSurobhi Lahiri, Byron Cole, Lakshmi Pulakat, Nara GaviniThe nitrogenase enzyme catalyzes the reduction of dinitrogen to ammonia and is composed of the Fe and MoFe proteins. The iron molybdenum cofactor (FeMo-co) of the MoFe protein is the site of active substrate reduction. The NifX protein has also been suggested to have a role in the FeMo-co synthesis, although its exact role is still open to investigation. We … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 2 · 30 Jun 2007 · 7,262 accessesView PDF Cite

Research ArticleOpen accessIn the abstractTracking Error Analysis of a Rotation-Elevation Mode HeliostatOmar Aliman, Ismail Daut, Muzamir Isa, Mohd Rafi AdzmanFor the past few years, great efforts have been done in improving the tracking accuracy of a newly proposed rotation-elevation tracking mode heliostat. A special simulation program has been developed to systematically analyze the image movement and to find out the error of the parameters. In the simulation program, ray-tracing method was applied to work out the central point position … …American Journal of Applied Sciences, Vol. 4, Issue 3 · 31 Mar 2007 · 7,251 accessesView PDF Cite

Research ArticleOpen accessIn the abstractGenetic Complementation Studies of Human Pin1 in Azotobacter vinelandii Revealed that it Requires Amino Terminus of the NifM to Deliver PPIase Effect to the Fe-protein of NitrogenaseKumaraguru Raja, Lakshmi Pulakat, Nara GaviniThe NifM is a peptidyl prolyl cis-trans isomerase and is required for the maturation and activation of the Fe protein of Nitrogenase. Since the carboxyl terminus of NifM is similar to the Human Pin1, we expressed the Human Pin1 in A. vinelandii BG98, a nifM mutant strain containing a kanamycin insertion and found that it could not complement the function … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 1 · 31 Mar 2006 · 6,708 accessesView PDF Cite