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9 articles for “K. V. Azarin”
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Research ArticleOpen accessIn the author namesSSR Analysis of the Chloroplast and Mitochondrial Genomes of Cultivar Lines and Wild Types of the Sunflower (Helianthus L.)Vladimir Arturovich Khachumov, Alexander Vyacheslavovich Usatov, Kirill Vitalievich Azarin et al.SSR analysis of chloroplast and mitochondrial genomes of cultivar lines and wild species of sunflower (Helianthus L.) were completed. The polymorphism of 18 microsatellite loci of the chloroplast and mitochondrial genomes was investigated. Based on the obtained data was constructed dendrogram showing the genetic diversity of chloroplast and mitochondrial genomes of sunflowers. The studied species were clustered into annual and … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 19 Feb 2020 · 7,675 accessesView PDFCite
Research ArticleOpen accessIn the author namesComparative Analysis of the Complete Chloroplast Genome of the Alloplasmic Sunflower (Helianthus L.) Lines with Various CMS TypesKirill Azarin, Maxim Makarenko, Alexander Usatov et al.The complete chloroplast genomes of sunflower fertile line HA89 and isonuclear CMS lines with four different cytoplasmic backgrounds (PET1, PET2, ANN2 and MAX) were sequenced. A total of 451 polymorphic sites, with including 58 SSRs, 317 SNPs and 76 microindels were revealed between the fertile and CMS cytotypes. Among the alloplasmic male-sterile lines, cpDNA of CMS-MAX had the largest number … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 1 · 2 Mar 2018 · 9,415 accessesView PDFCite
Research ArticleOpen accessIn the author namesThe Investigation of Organelle Genomes of Extra Nuclear Sunflower Mutants with Variegated PhenotypeIrina Dremuk, Nikolay Kozel, Mariya Logacheva et al.The comparative analysis of complete chloroplast and mitochondrial DNA sequences of the original inbred line 3629 and three extra nuclear mutant lines variegated-1, variegated-15, variegated-17, obtained by the N-nitroso-N-methylurea induced mutagenesis, was done. The studied mutant lines were presented two different phenotypes with diverse chlorophyll content: Pale/green mutant line variegated-17 with 1.8% relative (to 3629 line) chlorophyll content in mutant … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 24 Oct 2017 · 7,633 accessesView PDFCite
Research ArticleOpen accessIn the author namesValidation of SSR markers associated with Submergence Tolerance in Rice (Oryza sativa L.)Kirill V. Azarin, Alexander V. Usatov, Andrey V. Alabushev et al.The present work is devoted to the validation of SSR markers associated with submergence tolerance (Sub1A) QTLs in rice. It was shown that only microsatellite markers RM 7481 differentiates donor lines and recipient variety. Based upon the results of DNA analysis with the marker of the RM 7481 of the F2-plants, the 14 homozygotes by the locus Sub1A, 40 samples … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 4 · 19 Dec 2016 · 7,634 accessesView PDFCite
Research ArticleOpen accessIn the author namesEffects of Salt Stress on Ion Balance at Vegetative Stage in Rice (Oryza sativa L.)Kirill V. Azarin, Andrey V. Alabushev, Alexander V. Usatov et al.Rice (Oryza sativa L.) plants were subjected to NaCl stress within 35 days. The shoot dry weight, root dry weight, total survival percentage and contents of inorganic ions in dry samples of stressed plants were measured to identify the physiological adaptive mechanisms by which rice plants at vegetative stage tolerate salt stresses. Laboratory testing of the 10 rice cultivars showed … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 21 Mar 2016 · 7,464 accessesView PDFCite
Research ArticleOpen accessIn the author namesComparative Genomics of Domesticated and Wild Sunflower: Complete Chloroplast and Mitochondrial GenomesM. S. Makarenko, A. V. Usatov, N. V. Markin et al.The entire chloroplast and mitochondrial genomes of domesticated and wild type sunflower were sequenced. The comparative analysis of chloroplast genomes revealed 43 variant sites, including 21 polymorphic SSR loci and 22 SNPs. About 14 variant sites were found by collation of mitochondrial DNA (mtDNA), among them 4 SSRs, 8 SNPs and 2 deletions. About 9 SNPs were located in coding … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 29 Feb 2016 · 7,775 accessesView PDFCite
Research ArticleOpen accessIn the author namesIntrogression the SalTol QTL into the Elite Rice Variety of Russia by Marker-Assisted SelectionAlexander Vyacheslavovich Usatov, Andrey Vasilevich Alabushev, Pavel Ivanovich Kostylev et al.The present work is devoted to investigation of the possibility to apply methods of Marker-Assisted Selection (MAS) to introgression the SalTol QTL into genotypes of elite Russian varieties of rice. It was shown that microsatellite markers Rm493, may be effectively used to control transfer the SalTol QTL genes into Russian populations of rice. Based on the highly productive variety “Novator”, … …American Journal of Agricultural and Biological Sciences, Vol. 10, Issue 4 · 18 Nov 2015 · 9,259 accessesView PDFCite
Research ArticleOpen accessIn the author namesDNA-MARKERS OF SUNFLOWER RESISTANCE TO THE DOWNY MILDEW (PLASMOPARA HALSTEDII)A. V. Usatov, A. I. Klimenko, K. V. Azarin et al.One of the areas of biotechnology sunflower is the development and testing of DNA markers of important agronomic traits and in particular markers of resistance to downy mildew. Resistance of 16 Rf-lines of sunflower to the races 330 and 710 of Plasmopara halstedii has been studied. Genotyping of these lines was carried out using 9 STS-markers of three Pl-loci, Pl5, … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 2 · 24 Jul 2014 · 7,423 accessesView PDFCite
Research ArticleOpen accessIn the author namesTHE RELATIONSHIP BETWEEN HETEROSIS AND GENETIC DISTANCES BASED ON SSR MARKERS IN HELIANTHUS ANNUUSA. V. Usatov, A. I. Klimenko, K. V. Azarin et al.Identifying the best inbred combinations for the development of commercial hybrid of sunflower remains the main challenge to sunflower breeders. In the present research the level of heterosis of F1 hybrids, genetic diversity of parental lines based on SSR markers, as well as its connection with specific combining ability of sunflower were studied. Ten sunflower elite inbred lines (3 restorer … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 7 Mar 2014 · 8,003 accessesView PDFCite