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237 articles for “Isolates”
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Research ArticleOpen accessEvery word in the titlepH and Temperature Optimization of Several Bacterial Isolates from Mangrove Waters in the Mandeh Area to Produce Cellulase EnzymeFeskaharny Alamsjah, Dwiky Rahmadi Aswan, Anthoni AgustienEnzymes serve as vital biological catalysts, playing a crucial role in numerous industrial processes to accelerate specific biochemical reactions. In this study, we aimed to optimize cellulase enzyme production by investigating the influence of temperature and pH on four bacterial isolates: PUA-18, PUA-21, PUA-28, and PUA-38. The experiment was carried out in triplicates using a one-factor-at-a-time design, with pH and … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 28 Mar 2024 · 6,476 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleSELECTION OF BEAUVERIA BASSIANA (BALSAMO) VUILLEMIN ISOLATES FOR MANAGEMENT OF MYZUS PERSICAE (SULTZAR) (HOM.: APHIDAE) BASED ON VIRULENCE AND GROWTH RELATED CHARACTERISTICSMohammad S. Al-alawi, Maher ObeidatIsolates of the entomopathogenic fungus Beauveria bassiana originated from Jordan were evaluated for their efficacy against the green peach aphid, Myzus persicae under laboratory and greenhouse conditions. Efficacy evaluation involved testing all isolates at a concentration of 1×107 conidia/mL followed by concentration dependent and greenhouse bioassays for the top virulent isolates. Growth characteristics related to virulence were evaluated for high, … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 1 · 28 Dec 2013 · 7,943 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOptimization of Temperature and pH for Protease Production from Some Bacterial Isolates of Mangrove WatersFeskaharny Alamsjah, Yorasakhi Ananta, Anthoni AgustienProtease enzymes are widely used in biotechnology to produce amino acids and peptides from high molecular substrates, as well as in the leather industry for wastewater treatment, textiles, pharmaceuticals, cosmetics, leather processing, and poultry industry. Indonesia's demand for protease enzymes is increasing. This study aims to optimize the temperature and pH for the production of protease from certain bacterial isolates … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 11 Sep 2024 · 4,425 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIsolation and Characterization of Superior Symbiotic Bacterial Isolates from the Oenggae Sea with Potential as AntibacterialLukas Pardosi, Adelya Irawan Manalu, Charles Venirius LisnahanSponges are one of the marine biota that have a symbiotic relationship with bacteria which can produce secondary metabolites as antimicrobials. The main aim of this research was to isolate and identify bacteria that had potential as antibacterials against multi-drug resistant test bacteria. The sponsor samples used in this research were Stylissa massa, Salvador Dali, and Angelas sp. The methods … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 26 Mar 2024 · 5,053 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEnhanced Amylase Activity by Modulating Abiotic Factors and Enzyme Stability in the Thermophilic Bacterial IsolatesAnthoni Agustien, Denny Bendrianis, Feskaharny Alamsyah et al.Amylase is one of the enzymes that is widely used in bioprocess technology, amylase from different microbial sources also varies in form, so it is suitable for its specific application. The purpose of this study was to determine the optimum temperature, pH, agitation, and stability of the amylase enzyme from two thermophilic bacterial isolates, TUA-07 and TUA-09. This study used … …OnLine Journal of Biological Sciences, Vol. 24, Issue 2 · 7 Feb 2024 · 5,493 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleMycobacterium tuberculosis Complex Mutations in Drug Resistant Clinical Isolates from Southwest MexicoMartínez-Cruz Perla Mónica, Nakamura-López Yuko, Quintero-Hernández Verónica et al.Mutations in target genes have been described in Mycobacterium tuberculosis Complex (MTBc) drug resistant isolates worldwide. In Mexico, not enough information has been reported in this concern. The aim of this study was to characterize mutations related to resistance to first line drugs in MTBc isolates from Oaxaca, Mexico. MTBc isolates were identified in clinical samples from Tuberculosis (TB) patients. … …American Journal of Infectious Diseases, Vol. 17, Issue 4 · 28 Oct 2021 · 6,667 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDetection of tet Gene in Multidrug-Resistant Salmonella spp. Isolates from Layers and Broiler BreedersAgustin Indrawati, Leila Nur Azizah, I Wayan Teguh Wibawan et al.The present study was conducted with the following aims: (i) To detect the multidrug-resistant Salmonella spp. isolates recovered from faeces, litter and drinking water in layers and broiler breeders’ farms and (ii) to carried out the detection of the tet gene. A total of 21 Salmonella isolates were subjected to Polymerase Chain Reaction (PCR) assay to determine the presence of … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 2 · 17 Jun 2020 · 7,007 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleGenetic Diversity and Drug Resistance of Mycobacterium tuberculosis Complex Isolates and Nontuberculous Mycobacteria Identification from Presumptive Tuberculosis Cases in Oaxaca, MexicoNakamura-Lopez Yuko, Valencia-Carmona Oscar Daniel, Martinez-Cruz Perla Monica et al.Tuberculosis (TB) is a re-emerging health problem worldwide. In Mexico, information about genetic diversity and drug resistance of Mycobacterium Tuberculosis complex (MTBc) is limited. Samples from 463 Presumptive TB cases were tested for TB by smear, culture and PCR, from which 19.2% were identify as MTBc and 16% as nontuberculous mycobacteria. MTBc isolates were characterized by Large Sequence Polymorphisms (LSPs), … …American Journal of Infectious Diseases, Vol. 16, Issue 1 · 9 Feb 2020 · 7,159 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleExpression of microRNA miR-27, miR-124 and miR-218 Among Dental Pulp Stem Cell (DPSC) IsolatesMark Whiting, Karl KingsleyDental Pulp Stem Cells (DPSCs) are non-embryonic, mesenchymal stem cells that may have significant potential for therapeutic and regenerative biomedical applications. MicroRNAs are small non-coding RNA molecules that can act as transcriptional activators and repressors in many types of mesenchymal stem cells. To date, few studies have evaluated the expression or activity of microRNAs among dental pulp stem cells. Using … …Current Research in Dentistry, Vol. 9, Issue 1 · 1 Jan 2019 · 8,167 accessesView PDFCite
Literature ReviewOpen accessEvery word in the titleStaphylococcus aureus Vaccine Candidate from MRSA Isolates: The Prospect of a Multivalent VaccineNovra Arya Sandi, Tenri Ashari Wanahari, Isabel MacPhillamy et al.Staphylococcus aureus (S. aureus) is a major cause various infections in humans and animals throughout the world. The increasing incidence of S. aureus infection, particularly methicillin-resistant Staphylococcus aureus (MRSA) is complicated by the antibiotic-resistance phenomenon. Thus, it requires new strategies to prevent wider scale of its incidence occurring. Preventative methods against infectious diseases through vaccination can prevent, control and reduce … …American Journal of Infectious Diseases, Vol. 11, Issue 3 · 27 Aug 2015 · 8,471 accessesView PDFCite