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81 articles for “In vitro Method”

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Research ArticleOpen accessPhrase in the titleEvaluation of Digestibility of Palm Fronds Shaving (PFS) Supplemented with Vitamin B Complex on the Quality of Fermentation by in vitro Method and Degradation Kinetics by in Sacco MethodArmina Fariani, Gatot Muslim, Anggriawan Naidilah Tetra Pratama, Lili WarlyThis study aimed to determine the digestibility value, fermentation quality and degradation rate of palm frond shaving supplemented with vitamin B complex. The design used in this study was a Completely Randomized Design (CRD) consisting of three treatments and three replications. The treatments were as follows: Shaving palm fronds (CON), palm fronds shaving + vitamin B complex 1% (P1) and … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 10 Jun 2024 · 5,168 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInvestigation of Oocyte Retrieval Methods and Maturation Media on in vitro Development of Porcine EmbryosKayla M. Honneysett, Masindi L. Mphaphathi, Edward C. Webb, Ayanda MaqhashuThe objectives of the study were to: Compare oocyte retrieval methods (aspiration and slicing) from pig ovaries on oocyte quality and quantity; compare in vitro maturation (IVM) media (North Carolina State University -NCSU 37; Tissue Culture Medium-TCM 199; and porcine follicular fluid-mpFF) on oocytes polar body extrusion; compare the effect of Electrical Stimulation (ES) and frozen-thawed semen on cleavage rates … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 5 Jan 2022 · 6,600 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleLow-cost in vitro Screening Method for Digestibility of Pet ChewsTimothy J. Bowser, Charles I. Abramson, Dwayne BennettFour pet chew treats from four different manufacturers were tested for digestibility using in vitro tests in artificial gastric (stomach) and intestinal juices. In vitro tests were selected to determine the digestibility of dog chews for humane reasons and were conducted under conditions that were designed to simulate the digestive system of dogs. The purpose of the tests was to … …American Journal of Animal and Veterinary Sciences, Vol. 1, Issue 2 · 31 Dec 2006 · 7,552 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIsolation and Culture of Oocytes from Dairy Cows' Ovaries for Productivity in the Southeastern Region of KazakhstanDauren Maratovich Bekenov, Batyrkhan Azimkhanovich Buralkhiev, Yusupzhan Artykovich Yuldashbayev et al.This research work aims to study methods for isolating and culturing follicular oocytes from Holstein cows in vitro. The proven complex of technologies for embryo transplantation on an industrial scale will provide a 3-5-fold acceleration of reproduction of highly productive cows. As a result of scientific research, biotechnological methods of accelerated reproduction of dairy cattle have been substantiated and introduced … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 14 Oct 2024 · 4,298 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIn vitro and In vivo Activity of Lactococci Strains against Helicobacter pyloriHassina Guetarni, Ahmed Bensoltane, Djamila Aichour et al.Problem statement: Search for lactic acid bacteria that have in vitro, a significant inhibitory effect against the strains of H. pylori and to determine the inhibitory activity in vivo. Approach: The in vitro inhibitory activity of lactic acid bacteria isolated from milk against strains of H. pylori was determined by the agar diffusion method. Two groups of mice were inoculated … …OnLine Journal of Biological Sciences, Vol. 12, Issue 2 · 15 May 2012 · 7,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro Polyploid Induction in Phalaenopsis Aphrodite by PendimethalinPrasetyorini Djarot, Deden Sukmadjaja, Fadya Annisa Chaesarani, SukonoPhalaenopsis aphrodite is a type of orchid with high ornamental and economic value. However, its development for commercial needs in Indonesia is still hindered by the limited availability of varieties. Therefore, enhancing genetic diversity is necessary, one of which is through polyploid induction. Several previous studies reported that pendimethalin effectively duplicates the chromosome numbers of various plant species. The aim … …OnLine Journal of Biological Sciences, Vol. 26, Issue 3 · 22 Aug 2026 · 854 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro and in Silico Evaluation of Bioactive Compounds from Usnea longissima Ach. as Antimicrobial AgentsOky Kusuma Atni, Erman Munir, Nursahara Pasaribu et al.Lichens, formed from symbiotic association between of fungi with phototrophic organisms, generate a variety of bioactive chemicals having medicinal potential. This research aimed to examine the antibacterial characteristics of Usnea longissima Ach. using both in vitro and in silico methodologies. The effectiveness of lichen methanol extracts against different diseases was evaluated using the disc diffusion technique. Pathogens included gram-positive, gram-negative, … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 10 Feb 2026 · 1,721 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro Antimicrobial Activity of Modified Lysozyme (Lys 90) and Herbal Extracts Against Multidrug-Resistant Escherichia coliEni Kusumaningtyas, Bagem BR Sembiring, Andriani et al.The emergence of Antimicrobial Resistant (AMR) poses a significant challenge to the poultry farming industry. This study investigated the potential of Lysozyme (Lys), modified Lysozyme (Lys 90), an herbal extract, and their combination (herbs + Lys 90) as alternative antimicrobial agents against Multidrug-Resistant Escherichia coli (MDR-E. coli). Lys 90 was prepared by heating Lys at 90 °C for 20 minutes. … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 1 Dec 2025 · 2,076 accessesView PDFCite
Literature ReviewOpen accessIn the abstractControl of Contamination of Tissue Plant Cultures During in Vitro Clonal MicropropagationIrina Anikina, Nursultan Kaynidenov, Dora Dayu Rahma Turista et al.Plant micropropagation is currently an important method for modern large-scale propagation of valuable plant crops. Among the risks limiting the success of this method is the contamination of cultured plants. Conventional surface sterilization methods are not always effective against internal infection of plant tissues, and secondary contamination of tissue cultures during replication is also a major problem. This causes scientific … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 8 Apr 2025 · 8,693 accessesView PDFCite
Research ArticleOpen accessIn the abstractProtective Role of Ganoderma lucidum Polysaccharides Against Stress Induced by Heavy Metals in Caenorhabditis elegansHuixiu Ma, Dehui Dai, Guicai Chen, Weilian HuAli, H., Khan, E., & Ilahi, I. (2019). Environmental Chemistry and Ecotoxicology of Hazardous Heavy Metals: Environmental Persistence, Toxicity, and Bioaccumulation. Journal of Chemistry, 2019, 6730305. https://doi.org/10.1155/2019/6730305 Aljerf, L., & Aljerf, N. (2023). Food products quality and nutrition in relation to public. Balancing health and disease. Progress in Nutrition, 25(1), e2023024. https://doi.org/10.23751/pn.v25i1.13928 Aljerf, L., Williams, M., Ajong, A. B., Onydinma, … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 2 · 18 May 2024 · 6,947 accessesView PDFCite