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67 articles for “In vitro Culture”
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Research ArticleOpen accessPhrase in the titleEFFECT OF COPPER SULPHATE AND COBALT CHLORIDE ON GROWTH OF THE IN VITRO CULTURE TISSUES FOR DATE PALM (PHOENIX DACTYLIFERA L.) CV. ASHGARAhmed Madi Waheed AL-MayahiThis study was carried out to investigate the effects of copper sulphate and cobalt chloride on propagation of date palm cv. Ashgar in vitro. The rate of callus proliferation was significantly higher in the medium supplemented with 2 µM copper sulphate and 2 µM cobalt chloride together (p<0.05). Addition of copper sulphate and Cobalt chloride to the medium was most … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 1 · 27 Nov 2013 · 8,456 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn Vitro Prevention of Browning in Plantain CultureInnocent Chimereze Onuoha, Chinonye Jemimah Eze, Chibuikem I.N. UnambaProblem statement: The in vitro propagation of plantain (Musa paradisiaca) is still faced with lots of challenges such as blackening or browning of tissues prior to culture due to the oxidation of phenolic compound by polyphenolic oxidase enzyme present in the tissue when excised. Approach: Understanding browning processes in plantain and possible ways of minimizing it during excision of explants … …OnLine Journal of Biological Sciences, Vol. 11, Issue 1 · 6 Jul 2011 · 15,229 accessesView PDFCite
Literature ReviewOpen accessEvery word in the titleControl of Contamination of Tissue Plant Cultures During in Vitro Clonal MicropropagationIrina Anikina, Nursultan Kaynidenov, Dora Dayu Rahma Turista et al.Plant micropropagation is currently an important method for modern large-scale propagation of valuable plant crops. Among the risks limiting the success of this method is the contamination of cultured plants. Conventional surface sterilization methods are not always effective against internal infection of plant tissues, and secondary contamination of tissue cultures during replication is also a major problem. This causes scientific … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 8 Apr 2025 · 8,693 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePotential of HMEC-1 Line and HUVEC Primary Culture Cells to Study the Neonatal IgG Fc Receptor in vitroLuz Belinda Ortiz-Alegría, Irma Cañedo-Solares, Felipe Vadillo-Ortega et al.The neonatal IgG Fc receptor (FcRn) plays an important role in IgG homeostasis and immunity passive transfer. Fine points regarding these mechanisms, however, are still emerging. In order to obtain information about these phenomena, it is essential to have in vitro models of endothelium that express this receptor. In this study we chose two widely used models of human endothelial … …American Journal of Immunology, Vol. 12, Issue 1 · 21 Mar 2016 · 9,446 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Fetal Serum and Follicular Liquor Supplementation on the In Vitro Production of Bovine EmbryosClara Larocca, Yael Filipiak, William Perez, Juan CalvoTo optimize In Vitro Fertilization (IVF) results in bovines by lowering costs, comparing the efficiency of Fetal Calf Serum (FCS) (high cost) with respect to bovine Follicular Fluid (bFF) (low cost) and by quantifying embryo production. Cumulus-Oocyte-Complexes (COC) obtained from a slaughter house, transported at 37°C in saline solution, were classified according to their quality in A, B, C and … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 3 · 9 Aug 2012 · 7,839 accessesView PDFCite
Research ArticleOpen accessIn the keywordsBasal Salt Requirements Differ According to Culture Stage and Cultivar in Date Palm Somatic EmbryogenesisJameel M. Al-KhayriProblem statement: Various media formulations differing in basal salt composition are arbitrary selected to provide essential nutrients for plant in vitro cultures. Evidence suggests that in vitro response to various media formulations varies among genotypes and depends on the culture stage. This study examined the efficacy of five basal salt formulations on callus growth and somatic embryogenesis in date palm … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 1 · 27 May 2011 · 10,156 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEnhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake GrassWan Nurul Hidayah Wan Anuar, Suat-Hian Tan, Siti Hamidah Radiyah Shiekh Mahmud et al.Medicinal plants which is Clinacantus nutans (C. nutans) that known as Sabah Snake Grass or Belalai Gajah can treat several diseases including cancer. In order to provide and grow this plant in large number for health and pharmaceutical industry, one of the solutions is through the plant tissue culture technique. In this experiment, C. nutans was cultured under controlled conditions … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 31 Oct 2019 · 8,193 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro Polyploid Induction in Phalaenopsis Aphrodite by PendimethalinPrasetyorini Djarot, Deden Sukmadjaja, Fadya Annisa Chaesarani, SukonoPhalaenopsis aphrodite is a type of orchid with high ornamental and economic value. However, its development for commercial needs in Indonesia is still hindered by the limited availability of varieties. Therefore, enhancing genetic diversity is necessary, one of which is through polyploid induction. Several previous studies reported that pendimethalin effectively duplicates the chromosome numbers of various plant species. The aim … …OnLine Journal of Biological Sciences, Vol. 26, Issue 3 · 22 Aug 2026 · 854 accessesView PDFCite
Research ArticleOpen accessIn the abstractMarine-Derived Extracts as Economically Sustainable and Ethical Alternatives to Fetal Bovine Serum in HEK293 Cell CultureKian Kenneth Francisco Sanchez, Jennifer Seki-Wong et al.Fetal Bovine Serum (FBS) is a staple supplement in cell culture, providing key growth factors that promote cell health and proliferation. Since 1885, cell culture has been a cornerstone of both basic and applied research, leading to significant discoveries of cell communication and function. However, the use of FBS presents economic and ethical challenges. Millions of bovine fetuses are euthanized … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 7 Aug 2026 · 1,042 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of a Cyclachaena Xanthiifolia Extract on Trematodes Opisthorchis Felineus: An in Vitro StudyDenis Ponomarev, Elena Khramova, Natalia Gubanova et al.Cyclachaena xanthiifolia is a phytochemically rich invasive weed with documented allelopathic properties, making it a candidate source of bioactive compounds with potential medicinal applications. This study investigated the anthelmintic activity of a crude aqueous-ethanolic extract of C. xanthiifolia (CxEt), prepared from equal proportions of leaves and seeds, against the trematode Opisthorchis felineus in vitro. The effects of CxEt on the … …OnLine Journal of Biological Sciences, Vol. 26, Issue 2 · 1 Jun 2026 · 1,251 accessesView PDFCite