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10 articles for “Immobilized Enzyme”
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Research ArticleOpen accessIn the keywordsPotential Applications of Chitosan Nanoparticles as Novel Support in Enzyme ImmobilizationHoda Jafarizadeh Malmiri, Mohammad Ali Ghaz Jahanian, Aydin BerenjianChitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 11,302 accessesView PDFCite
Research ArticleOpen accessIn the abstractPotentiometric Biosensor for Highly Toxic Pb (lead) and Cd (cadmium) Detection in Milk Using Layer-by-Layer ImmobilizationZhainagul Kakimova, Klara Zharykbasova, Aitbek Kakimov et al.Milk contamination with heavy metals such as lead (Pb) and Cadmium (Cd) poses a serious threat to public health, particularly in industrial regions. To address the need for rapid and on-site detection of these contaminants, a potentiometric biosensor was developed for the quantification of Pb²⁺ and Cd²⁺ ions in milk and dairy products. The biosensor functions by measuring the change … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 8 Feb 2026 · 1,480 accessesView PDFCite
EditorialOpen accessIn the abstractNeutrophil Extracellular Traps as Therapeutic Targets for Inflammatory DiseaseRory R. KoenenNeutrophilic granulocytes, or neutrophils are the most abundant circulating white blood cell and constitute a first line of defense against pathogens. They are characterized by a large polymorphic lobular nucleus and the presence of many secretory granules in their cytoplasm. Neutrophils are essential for a functional host defense, as is evidenced by the occurrence of severe infections in individuals with … …American Journal of Pharmacology and Toxicology, Vol. 9, Issue 4 · 25 Feb 2015 · 8,737 accessesView PDFCite
Research ArticleOpen accessIn the abstractLIPASE-CATALYZED TRANSESTERIFICATION OF PALM KERNEL OIL WITH DIALKYLCARBONATESTjahjono Herawan, M. Rüsch Gen. KlaasLipase-catalyzed transesterifications-especially in a solvent-free medium-are important for industrial applications because such systems would have an enormous advantage by avoiding the problem of separation, toxicity and flammability of organic solvents. However, the organic solvent-free alcoholysis, especially methanolysis, does not give high conversions. The same problem also occurs when ethyl or methyl acetate are used as acyl acceptors. The main problems … …American Journal of Applied Sciences, Vol. 11, Issue 8 · 6 May 2014 · 6,793 accessesView PDFCite
Research ArticleOpen accessIn the abstractOPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORMNayera A.M. Abdelwahed, Enas N. Danial, Noura El-Ahmady El-Naggar, Asem A. MohamedOptimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL-1 were 30°C at pH 8.5 and incubation time 96 h in free cell cultures using starch 20 g L-1 as carbon source and yeast extract 5 g L-1 … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Dec 2013 · 8,125 accessesView PDFCite
Research ArticleOpen accessIn the abstractTailor-Made Enzyme Carriers: Preparation and Use of Adsorbents Specifically Designed to Immobilize Allosteric Enzymes in Activated ConformationZahra SalemiProblem statement: The enzyme immobilization has experienced substantial growth in the recent past and an ever increasing amount of study has been reported on various aspects of immobilized enzymes. In most of these investigations, catalytic activities are found to be diminished as compared to the enzyme free in solution. Approach: Hydrophobic adsorbents were prepared containing L-leucine or citric acid, two … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 7,558 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization and Wash Performance Analysis of Microbial Extracellular Enzymes from East Calcutta Wetland in IndiaRamesh Malathu, Sanhita Chowdhury, Madhusmita Mishra et al.Extracellular protease from a novel bacterial isolate showing maximum similarity of 98.22% with Microbacterium luteolum was obtained from East Calcutta Wetland, India. It showed compatibility with commercial detergents. The enzyme retains more than 60% of its activity between 6.0 to 10.5 pH. The maximum activity is at pH 7.5 with 71% activity at pH 10.0 and 10.5. The protease retained … …American Journal of Applied Sciences, Vol. 5, Issue 12 · 31 Dec 2008 · 8,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmobilization of Lipase from Candida Rugosa on Palm-Based Polyurethane Foam as a Support MaterialRoila Awang, Mohd Rafaei Ghazuli, Mahiran BasriLipase from Candida rugosa was immobilized onto palm-based polyurethane foam (PU), which the polymer was pre-soaked in co-immobilized agent. The activities of PU-immobilized lipase were tested by the esterification reaction of oleic acid and oleyl alcohol in hexane. The PU-immobilized lipase was then characterized in term of its thermal, operational and storage stability. The optimum temperature for native and PU-immobilized … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 7,478 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiosensor Based on Xanthine Oxidase for Monitoring Hypoxanthine in Fish MeatHelena S. Nakatani, Luciane V. dos Santos, Cristiano P. Pelegrine et al.An amperometric enzyme electrode for the detection of hypoxanthine in fish meat is described. The hypoxanthine sensor was prepared from xanthine oxidase immobilized by covalent binding with glutaraldehyde and bovine serum albumin on a nafion-coated platinum disc electrode. The responses for hypoxanthine were obtained in 0.1 mol L-1 phosphate buffer (pH 7.0), at potential of 600mV vs Ag/AgCl. This biosensor … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 8,597 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Development of a New, Rapid, Amperometric Immunosensor for the Detection of Low Concentrations of Bacteria Part II: Optimization of the System for Escherichia coliEric Carnes, Ebtisam WilkinsThe optimization of a flow-through immunoassay system was conducted to achieve rapid detection of low concentrations of E. coli bacteria. The system includes a new immunosensor that uses newly developed, disposable immunofiltration membranes to yield a system that is sensitive as well as simple. Comparison of the performance of this system with conventional immunoassays demonstrated a significantly shorter time for … …American Journal of Applied Sciences, Vol. 2, Issue 3 · 31 Mar 2005 · 6,780 accessesView PDFCite