2 articles for “Genetic complementation”

Research ArticleOpen accessPhrase in the titleGenetic Complementation Studies of Human Pin1 in Azotobacter vinelandii Revealed that it Requires Amino Terminus of the NifM to Deliver PPIase Effect to the Fe-protein of NitrogenaseKumaraguru Raja, Lakshmi Pulakat, Nara GaviniThe NifM is a peptidyl prolyl cis-trans isomerase and is required for the maturation and activation of the Fe protein of Nitrogenase. Since the carboxyl terminus of NifM is similar to the Human Pin1, we expressed the Human Pin1 in A. vinelandii BG98, a nifM mutant strain containing a kanamycin insertion and found that it could not complement the function … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 1 · 31 Mar 2006 · 6,708 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDiscovery of Evolutionary Divergence of Biological Nitrogen Fixation and Photosynthesis: Fine Tuning of Biogenesis of the NifH and the ChlL by a Peptidyl-Prolyl Cis/Trans IsomeraseNara Gavini, Sinny Delacroix, Kelvin Harris Jr., Lakshmi PulakatProblem statement: Despite the structural and functional similarities between the nitrogenase that performs biological nitrogen fixation reaction and the Dark Protochlorphyllide Oxidoreductase (DPOR) that performs chlorophyll-biosynthesis, attempts to substitute nitrogenase-components with DPOR-components have hitherto failed. This investigation was undertaken to test if Chlamydomonas reinhardtii protochlorophyllide (Pchlide) reductase (ChlL) that shares some structural similarity with Nitrogenase Reductase (NifH) could complement the … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 17 Feb 2012 · 7,614 accessesView PDF Cite