4 articles for “Genetic Transformation System”

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Research ArticleOpen accessIn the keywordsKnock Down of Erythrulose Kinase (eyk1) Leads to the Enhancement of Erythritol Production in Yarrowia lipolytica Mutant Strain YE4-2Xinhe Zhao, Tianlong Huang, Yaxin Zhang et al.Erythritol is a nearly zero-calorie sweetener, which is in great demand in food and health care industry. Our previous work obtained a Yarrowia lipolytica mutant strain YE4-2 with high erythritol yield. However, its conversion rate of glucose needs further improvement. Erythrulose kinase (eyk1) participates in the early stages of erythritol catabolism; the existence of eyk1 prevents the accumulation of erythritol. … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 3 · 24 Jul 2020 · 6,750 accessesView PDF Cite

Research ArticleOpen accessIn the abstractBidirectional Blockchain-Based Secure Data Transfer Using GALOEEM Routing Protocol in WSNAnitha Rajakumari Ponjothi, Pritee ParwekarData exchange is hampered by the fact that warehouse data are distributed among warehouses. To propose a secure bidirectional blockchain-based data-sharing system that collects warehouse data and controls data sharing through bidirectional transformation. This study is proposed for establishing an efficient bi-directional data warehouse storage for script less blockchains with an infinite lifespan. The sensor provides the data used in … …Journal of Computer Science, Vol. 19, Issue 8 · 20 Jul 2023 · 5,815 accessesView PDF Cite

Research ArticleOpen accessIn the abstractProposal of a Genome Editing System for Genetic Resistance to Tomato Spotted Wilt VirusFederico Martinelli, Giovanni Grillone, Filippo SgroiViruses provoke considerable economical losses in agriculture. New molecular approaches to develop genetic resistance based on translational genomics and precision genetic modifications are highly expected. The type II Clustered, Regularly Interspaced Palindromic Repeats (CRISPR) system including Cas9 nuclease represent a promising and very powerful tool to specifically modulate the expression and activity of genes involved in biotic stress responses. In … …American Journal of Applied Sciences, Vol. 11, Issue 11 · 7 Jan 2015 · 7,685 accessesView PDF Cite

Research ArticleOpen accessIn the abstractThe Supervirulence Plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation Efficiency of Hevea brasiliensisP. Arokiaraj, R. Leelawathy, H. Y. YeangProblem statement: The present study investigates the ability of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformation using two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum albumin cDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens A281. The transformed callus … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 8,920 accessesView PDF Cite