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13 articles for “Embryogenesis”

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Research ArticleOpen accessEvery word in the titleSomatic Embryogenesis of Oil Palm (Elaeis guineensis Jacq.) from Bud Explants Using Suspension CultureNurul Huda Panggabean, Isnaini Nurwahyuni et al.The propagation of oil palm seeds through tissue culture and somatic embryogenesis is a preferred method due to its ability to produce entire plants without the need for gamete fusion. While somatic embryo proliferation is typically performed on solid media, this approach is less efficient than liquid media, which is both more cost-effective and can lead to better proliferation results. … …OnLine Journal of Biological Sciences, Vol. 23, Issue 2 · 4 May 2023 · 5,863 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBasal Salt Requirements Differ According to Culture Stage and Cultivar in Date Palm Somatic EmbryogenesisJameel M. Al-KhayriProblem statement: Various media formulations differing in basal salt composition are arbitrary selected to provide essential nutrients for plant in vitro cultures. Evidence suggests that in vitro response to various media formulations varies among genotypes and depends on the culture stage. This study examined the efficacy of five basal salt formulations on callus growth and somatic embryogenesis in date palm … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 1 · 27 May 2011 · 10,156 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInvestigation of the Contribution of Late Embryogenesis Abundant (LEA) K Peptide in Enhancing the Expression of Lipase from Sphingobacterium sp.: In vitro and in Silico StudiesIbrahim Muhammad et al.Sphingobacterium sp. AB3 lipase is a psychrophilic enzyme with optimal lipolytic activity at pH 7 and 15°C. The cold-adapted properties of the lipase render it suitable for various low-temperature industrial applications such as biodiesel production, detergent formulation, and non-thermal food processing. Recent studies have shown that co-expression of LEA K peptide with AB3 lipase resulted in enhanced protein expression in … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 21 Nov 2024 · 4,569 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L )Abdullatif AlkhateebThe effect of different concentration of date palm syrup (2, 4, 6, 8 and 10%) and sucrose at concentration of 30 and 60 g/l in addition to the control (without carbon source) on the micro propagation of date palm "cv. Suckary" were investigated. The results indicated that date syrup was taken up from the media as shown by the increase … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 1 · 31 Mar 2008 · 7,982 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMorphogenesis in Tissue Culture of Local Kazakh Cotton VarietiesBishimbayeva Nazira, Yertayeva Bakhyt, Amirova Aigul, Rakhimbayev IzbasarCallus was initiated from hypocotyl and cotyledon explants of seven of commercial Kazakh cotton cultivars on MS medium supplemented with 0,1 mg L-1 2,4-D and 0,5 mg L-1 kinetin. The percentage of callus formation ranged from 64,0 to 91,0% for cotyledons and from 72 to 90% for hypocotyls. Study of morphological heterogeneity peculiarities of metamorphosis and histological structure of calli … …OnLine Journal of Biological Sciences, Vol. 17, Issue 3 · 12 Jul 2017 · 7,171 accessesView PDFCite
Review ArticleOpen accessIn the keywordsDNA Vaccination Associated with Eliminating Inheritance Risks during Embryo Development: A ReviewRia MargianaThe process of immunization using modified DNA is essential whenever scientists desire to trigger an artificial response in the host’s body to improve immunity. The use of DNA vaccines awakens the somatic cells of the host to produce antigens that will assist the body to prepare to fight infections through preventive measures triggered by the vaccines. These biological inventions have … …OnLine Journal of Biological Sciences, Vol. 16, Issue 2 · 26 May 2016 · 6,873 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Mother Plant and Somatic Embryo Registry for the Propagation and Conservation of Artificial Seeds of the Orchid Phragmipedium kovachiiOsber Cunia, Manuel Oliva, Segundo G. Chavez et al.Phragmipedium kovachii, is an endemic orchid that naturally inhabits the humid zones of the Amazonas region in Peru. Currently, it has been extracted from its natural habitat, listed as critically endangered by CITES, suggesting searching for conservation strategies in vitro and in situ. Therefore, this research established an in vitro protocol to obtain and conserve artificial seed, through four events: … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 8 Jul 2024 · 5,302 accessesView PDFCite
Research ArticleOpen accessIn the abstractATRA AS A POTENTIAL ANTIMETASTATIC AGENT IN COLORECTAL ADENOCARCINOMARobert Edwards, Kambiz Afrasiabi, Kehui WangThe family of Retinoic acid compounds have long been the subject of intense study and interest as chemo prophylactic agents. This dates back to their therapeutic effect in leukoplakia Where ATRA was shown to lead to the reversal of premalignant lesions and Subsequent reports on their use in metastatic colorectal adenocarcinoma and a vast array of other malignant disorders as … …OnLine Journal of Biological Sciences, Vol. 14, Issue 2 · 26 Mar 2014 · 7,344 accessesView PDFCite
Research ArticleOpen accessIn the abstractSCREENING FOR CUCUMBER MOSAIC RESISTANT LINES FROM THE OVULE CULTURE DERIVED DOUBLE HAPLOID CUCUMBERSParichat Plapung, Sirisupaporn Khamsukdee, NutthaPotapohn, Prasartporn SmitamanaCMV is one of the major destructive viruses worldwide and commercial CMV resistant cucumber is very rare. Therefore we aimed to establish the ovule derived resistant lines used for the breeding program. Haploid plants of sixty-eight cucumber lines were successfully obtained by culturing the un pollinated ovaries harvested one day before an thesis on a modified MS medium supplemented with … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 28 Feb 2014 · 7,903 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Supervirulence Plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation Efficiency of Hevea brasiliensisP. Arokiaraj, R. Leelawathy, H. Y. YeangProblem statement: The present study investigates the ability of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformation using two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum albumin cDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens A281. The transformed callus … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 8,920 accessesView PDFCite