Research ArticleOpen accessIn the keywordsOptimization of Hydrolysis Conditions for Obtaining Antithrombotic Peptides from Tenebrio Molitor LarvaeFangyuan Chen, Han Jiang, Yini Gan et al.Tenebrio molitor larvae was hydrolyzed by pepsin and trypsin for preparing high antithrombotic activity hydrolysate. The significant variables were selected according to the Plackett-Burman design and further optimized by the Response Surface Methodology (RSM). Four factors, including substrate concentration, pepsin digestion time, pepsin amount and trypsin amount, respectively, influenced significantly on the hydrolysis effectiveness. The best hydrolysis conditions obtained using … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 20 May 2019 · 8,722 accessesView PDFCite