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53 articles for “Affinity”
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Research ArticleOpen accessEvery word in the titleEnhancement of Geobacillus thermodenitrificans Lipase Activity and Binding Affinity by Acetone Treatment: Kinetic and in silico InvestigationsAmmar Khazaal Kadhim Almansoori, Alyaa Abdulhussein Alsaedi et al.Lipase Geobacillus thermodenitrificans (LipGt) is a thermostable enzyme that has an optimal activity temperature of 65°C. It can tolerate various organic solvents and has an improved activity, especially in the presence of acetone. In this study, in-vitro and in-silico studies were carried out to investigate the effect of acetone in enhancing the kinetic and binding affinity of LipGt. The LipGt … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 10 Oct 2024 · 4,817 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInfluence of Tags on the Binding Affinity of Acyl-CoA Binding ProteinJizhong Han, Yunlong Sun, Yu Chen et al.Tagged fusion proteins are frequently employed for protein purification methods, but their effects on protein function and binding affinity are rarely studied. Here we expressed recombinant protein Acyl-CoA Binding Protein (ACBP) cloned from the full-length cDNA of Aspergillus oryzae and Saccharomyces cerevisiae. ACBP was expressed in Escherichia coli fused to a Maltose-Binding Protein (MBP) and Histidine-tag fusion. Recombinant ACBP was … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 23 Jan 2019 · 9,097 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAssessment of Taxonomic Affinity of Indonesian Pummelo (Citrus maxima (Burm.) Merr.) Based on Morphological CharactersRatna Susandarini, Siti Subandiyah, Rugayah et al.Citrus maxima is a tropical fruit species with high morphological variability on fruit characters, including shape, size, thickness of peel, flesh color and taste. This variability has been used by breeders and common people to practically distinguish different pummelo cultivars. Despite such well known variation, so far there is no published study specifically addressed the extent of phenotypic variability and … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 3 · 4 Sep 2013 · 8,538 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAssociation of High-Affinity FcεRI-β intron 2 for IgE Gene Polymorphism and Atopic Diseases in an Algerian PopulationDahbia Ines Dahmani, Ikhlass Haj Salem, Jamila Chakir et al.Atopy is characterized by the production of a high level of IgE in response to common allergens. We examined the RsaI-in2 polymorphism of the FcεRI-β gene and its association with atopic asthma and comorbidity. The study population included 77 atopic asthmatics and 77 non-atopic, non-allergic and non-asthmatic controls. Atopic asthma was confirmed by the skin prick test and by IgE … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 1 Nov 2017 · 7,444 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCYCLIC CHOLECYSTOKININ ANALOGUES EXHIBIT HIGH BLOOD STABILITY AND BINDING AFFINITY WITH CHOLECYSTOKININ RECEPTOREun-Ha Joh, Jae Cheong Lim, Jin Joo Kim et al.Recently, incidence of Cholecystokinin (CCK) receptor is recognized as a factor that determines the aggressive phenotype of pancreatic cancer. In this study, a novel Cholecystokinin (CCK) analogues; DOTA-Nle-cyclo (Glu-Trp-Met-Asp-Phe-Lys-NH2) (DOTA-cCCK) and DOTA-Nle-cyclo (Glu-Trp-Nle-Asp-Phe-Lys-NH2) (DOTA-[Nle]-cCCK) were synthesized and radiolabeled and the targeting abilities on the CCK receptor were evaluated for new CCK receptor targeting agents searching. Peptides were prepared through a … …OnLine Journal of Biological Sciences, Vol. 14, Issue 1 · 2 Jan 2014 · 7,556 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Effect of NaCl on the Stability of Ab3 Lipase from Sphingobacterium sp. that Co-Expressed with LEA K Using In silico ApproachIbrahim Muhammad et al.This study explores how sodium chloride (NaCl) influences the binding affinity and stability of the Ab3 lipase-LEA K complex when interacting with olive oil, using molecular docking techniques. The docking simulations, conducted with AutoDock Vina, reveal that the presence of NaCl significantly enhances the binding affinity of the enzyme-substrate complex, increasing it from-6.55 kcal/mol in the absence of salt to-7.81 … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 9 Mar 2025 · 5,599 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEmerging Trends in Designing Short and Efficient Protein Purification ProtocolsSaurabh Gautam, Joyeeta Mukherjee, Ipsita Roy, Munishwar Nath GuptaProtein purification is carried out in both academic and industrial sectors. Both non-chromatographic approaches (precipitation, crystallization, partitioning) and chromatographic methods are used. When recombinant proteins are over expressed in foreign hosts, inclusion bodies are obtained. Many purification methods can be used to refold proteins. Many strategies allow one to avoid formation of inclusion bodies. The concept of affinity based separation … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 9,007 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCharacteristic Study of the Marine Algae Sargassum sp. on Metal AdsorptionA. Saravanan, V. Brindha, Soundarajan KrishnanProblem statement: Biomass of brown marine macro algae is a biological resource that is available in large quantities and can form a good base for the development of biosorbent material. Approach: Algae have been found to be potentially suitable biosorbents because of its cheap availability, both in fresh or salt water, relatively high surface area and high binding affinity. Results: … …American Journal of Applied Sciences, Vol. 8, Issue 7 · 24 Jun 2011 · 7,660 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIgG Subclass Variation of a Monoclonal Antibody Binding to Human Fc-Gamma ReceptorsRekha Patel, Krista K. Johnson, Bruce A. Andrien, Paul P. TamburiniThe importance of human Fc receptors in immune regulation is well known. Their role is critical not only in the recruitment of cellular effector functions but also in regulating the balance in the periphery between autoimmunity and tolerance. Despite their central importance, there is a dearth of literature on controlled numeric comparisons in affinities of antibody subclasses for gamma receptors. … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 8,065 accessesView PDFCite
Research ArticleOpen accessIn the abstractVirtual Screening of Bioactive Compounds from the Onion (Allium) Genus as Potential Epidermal Growth Factor Receptor Inhibitors for MonkeypoxDinda Ratu Agustiani, Rakhmat Ramdhani Alwie, Harry NoviardiMonkeypox (Mpox) is an emerging zoonotic disease caused by the Mpox virus infection that affects humans and animals. Epidermal Growth Factor Receptor (EGFR) is an important target in the pathogenesis of Mpox. Epidermal Growth Factor (EGF), encoded by orthopoxviruses, plays an important role in intercellular transmission of the virus. Onion is known to have antiviral and immunomodulatory properties. Active compounds … …OnLine Journal of Biological Sciences, Vol. 26, Issue 3 · 6 Aug 2026 · 945 accessesView PDFCite