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51 articles for “3C protease”
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Research ArticleOpen accessEvery word in the titleOptimization of Temperature and pH for Protease Production from Some Bacterial Isolates of Mangrove WatersFeskaharny Alamsjah, Yorasakhi Ananta, Anthoni AgustienProtease enzymes are widely used in biotechnology to produce amino acids and peptides from high molecular substrates, as well as in the leather industry for wastewater treatment, textiles, pharmaceuticals, cosmetics, leather processing, and poultry industry. Indonesia's demand for protease enzymes is increasing. This study aims to optimize the temperature and pH for the production of protease from certain bacterial isolates … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 11 Sep 2024 · 4,425 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOptimization and Molecular Identification of Protease-Producing Thermophilic Bacterial Isolate TUA-26Anthoni Agustien, Mufidhatul Muqarramah, Feskaharny AlamsjahProtease enzymes are used as biocatalysts that can accelerate a reaction. Thermostable enzymes produced by thermophilic bacteria are needed in the industrial field because of their ability to withstand high temperatures. The thermophilic bacterial isolate TUA-26 that produces protease was to analyze the optimum conditions of temperature, pH, agitation, trace elements, carbon sources, and nitrogen sources; the stability of protease … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 6 Apr 2024 · 4,935 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleMolecular Characterization and Differentiation of Proteases Isolated from Different Aspergillus Fungal SpeciesSamia Abd Allah Abdal-Aziz, Safaa M. AliA comparative molecular study of Aperigiullus protease using sequence of protease gene representative 25 different Aspergillus species (Aspergillus fumigatus, Aspergillus oryzae, Aspergillus flavus, Aspergillus novofumigatus, Aspergillus viridinutans, Aspergillus pseudotamarii, Aspergillus clavatus, Aspergillus sojae, Aspergillus fischeri, Aspergillus caelatus, Aspergillus novofumigatus, Aspergillus campestris, Aspergillus costaricaensis, Aspergillus candidus, Aspergillus neoniger, Aspergillus mulundensis, Aspergillus ibericus, Aspergillus niger, Aspergillus piperis, Aspergillus eucalypticola, Aspergillus vadensis, Aspergillus … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 11 Feb 2021 · 6,649 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleHide Dehairing and Laundry Detergent Compatibility Testing of Thermostable and Solvents Tolerant Alkaline Protease from Hot Spring Isolate Bacillus cohniiU3Pravin R. Dudhgara, Bhavsar Sunil, Ghelani AnjanaIn the present study, alkaline protease producing thermophilic Bacillus cohniiU3 strain was isolation from Unnai hot spring, India. The maximum production of protease (344 U/mL) was reported after 72 h of inoculation at 50°C in shake flask culture using the gelatin casein medium. The protease was found to remain active up to 96 h and production was growth dependent. The … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 23 Jul 2015 · 7,335 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORMNayera A.M. Abdelwahed, Enas N. Danial, Noura El-Ahmady El-Naggar, Asem A. MohamedOptimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL-1 were 30°C at pH 8.5 and incubation time 96 h in free cell cultures using starch 20 g L-1 as carbon source and yeast extract 5 g L-1 … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Dec 2013 · 8,125 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDegumming of Raw Silk Fabric with Help of Marine Extracellular ProteaseDas Sumana, Mathummal Sudarshan, Ashoke Ranjan Thakur, Shaon RayChaudhuriProtease secreting microbe was isolated and characterized on the basis of their morphological, biochemical, physiological and 16S rDNA based molecular properties. The extracellular protease was quantified and characterized. Protease was used for different time (4, 8, 12 and 24 h) at different temperature (RT and 37°C) for optimization of the degumming process for raw silk fabric with enzyme dosage (0.2-1 … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 1 · 18 Mar 2013 · 8,498 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleHigh Frequency of Stop Codons in the Human Immunodeficiency Virus-1 Protease Gene Frame in Human Immunodeficiency Virus+ Individuals with Below Detectable Levels of Plasma Viremia During Highly Active Antiretroviral TherapyMagdalena Czubala, Kabo Matlho, Maria Arriaga et al.We performed sequence analysis of HIV-1 proviral protease gene fragment (560 base pairs) amplified from ex-vivo peripheral blood mononuclear cells of 83 HIV+ individuals with Below Detectable Levels (BDL) (<20-40 RNA copies/ml plasma) and detectable levels of plasma HIV viremia while on HAART. Noteworthy was the systematic presence of stop codons identified only in the BDL group and not in … …American Journal of Infectious Diseases, Vol. 8, Issue 3 · 14 Nov 2012 · 8,073 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBACTERIAL ISOLATES OF MARINE COAST AS COMMERCIAL PRODUCER OF PROTEASES. Das, I. Mukherjee, M. Sudarshan et al.The objective of this work was to explore and exploit the extracellular protease secreting marine microbial biodiversity of the eastern coastal region of India. Culture dependent method was applied for isolation of microbes from the marine coast of West Bengal (Digha and Mandarmani) and Andhra Pradesh (Vizag) in India. Six protease secreting isolates were screened using casein hydrolysing property as … …OnLine Journal of Biological Sciences, Vol. 12, Issue 3 · 8 Aug 2012 · 8,795 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAnalysis of Resistance to Human Immunodeficiency Virus Protease Inhibitors Using Molecular Mechanics and Machine Learning StrategiesA. Harishchander, D. Alex Anand, Sanjib SenapatiProblem statement: Drug resistance is the most important factor, which influences the failure of current HIV therapies. So, the ability to predict the drug resistance of HIV-1 protease mutants will be useful in developing more effective and longer lasting treatment regimens. Approach: Drug resistance of HIV-1 protease is predicted with two current protease inhibitors (Indinavir and Saquinavir). The problem was … …Current Research in Medicine, Vol. 1, Issue 2 · 31 Oct 2010 · 7,204 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Effects of Protease Inhibitors on Metabolic Complications and Lipodystrophy in Human Immunodeficiency Virus-Infected PatientsMohd Khaza, K. Laxmi Prasad, M. EstariProblem statement: Highly Active Antiretroviral Therapy (HAART)-associated metabolic complications include lipoatrophy (loss of Subcutaneous Adipose Tissue (SAT) and insulin resistance. The risk of Diabetes Mellitus (DM) in Human Immunodeficiency Virus (HIV)-infected patients receiving Highly Active Antiretroviral Therapy (HAART) especially protease inhibitors has not been well defined. Approach: We conducted an analysis in the Hospital of Rajeev Gandhi Institute of Medical … …Current Research in Medicine, Vol. 1, Issue 2 · 31 Oct 2010 · 6,934 accessesView PDFCite