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94 articles for “primer”
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Research ArticleOpen accessIn the abstractOccurrence of Feline Immunodeficiency Virus and Feline Leukemia Virus Infection in CatsFabiana Alves, Daniela de Souza Rajao, Helen Lima Del Puerto et al.Problem statement: The objective of the present study was to evaluate occurrence of the Feline Immunodeficiency Virus (FIV) and the Feline Leukemia Virus (FeLV) infection in asymptomatic domestic cats in the metropolitan region of Belo Horizonte, Minas Gerais, using the Polymerase Chain Reaction (PCR) for FIV detection and SNAP Combo Plus for FeLV and FIV detection. Approach: Blood samples were … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 3 · 2 Nov 2011 · 8,717 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCRMohamed A. Nagaty, Salah El-Din El-Assal, Mahmoud M. RifaatProblem statement: Peach genotypes from the Kingdom of Saudi Arabia were not adequately characterized at the biological and molecular levels. This study was carried out to characterize nine peach cultivars growing in Taif (KSA) at the biological and molecular levels. Approach: For the nine peach cultivars studied, flowering and fruiting indices were determined and eight, 10-base primers were used to … …American Journal of Applied Sciences, Vol. 8, Issue 7 · 24 Jun 2011 · 7,271 accessesView PDFCite
Research ArticleOpen accessIn the abstractEarly Diagnosis of Breast Cancer using Molecular, Biochemical and Pathological MarkersSalah El-Din El-Assal, Adel A. El-Tarras, Samir M. Abd-allaProblem statement: Laboratory diagnosis of breast cancer in most of the hospitals has traditionally been performed using cell culture and the direct hormone receptor assay, which are money and time consuming. Approach: This study was performed in order to direct the attention toward increasing the efficiency of early diagnosis in clinical laboratories at the western region of KSA and Egypt … …American Journal of Applied Sciences, Vol. 8, Issue 1 · 31 Jan 2011 · 8,056 accessesView PDFCite
Research ArticleOpen accessIn the abstractCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,724 accessesView PDFCite
Research ArticleOpen accessIn the abstractSome Effects of Abiotic Stress on Infection of Dyer’s Woad (Isatis tinctoria L.) by Puccinia thlaspeos C. Schub.: Implications for Biological ControlElizabeth Thomas, Bradley Ray KroppProblem statement: The rust pathogen, Puccinia thlaspeos, is being studied as a potential biocontrol agent for the noxious weed dyer’s woad. Although its disease etiology is well understood, very little has been learned about the effect of environmental stresses on infection. Approach: Dyer’s woad plants were exposed to different levels of oxidative stress, salinity stress, osmotic stress, dehydration, and cold … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 1 · 15 Dec 2010 · 8,715 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent CompostAzhari Samsu Baharuddin, Mohamad Nafis Abd Razak, Lim Siong Hock et al.Problems statement: Lack of information on locally isolated cellulase-producing bacterium in thermophilic compost using a mixture of Empty Fruit Bunch (EFB) and Palm Oil Mill Effluent (POME) as composting materials. Approach: The isolation of microbes from compost heap was conducted at day 7 of composting process where the mixture of composting materials consisted of 45.8% cellulose, 17.1% hemicellulose and 28.3% … …American Journal of Applied Sciences, Vol. 7, Issue 1 · 31 Jan 2010 · 12,155 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization of Bovine Calpastatin Gene in Nelore Cattle Using Polymerase Chain Reaction-Restricted Fragment Length PolymorphismsAlireza Majidi, Jothi Malar Panandam, Awis Qurni Sazili, Siti Shapor SirajProblem statement: In beef cattle production, of meat quality and carcass traits are important. Traditionally beef cattle breeding programs unfortunately are time consuming and also recording of carcass and growth traits need heavy cost, Approach: Marker Assisted Selection (MAS) should be utilized in beef herds, along with economically important phenotypic traits, for genetic progress to made with respect to improving … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 4 · 31 Dec 2009 · 7,329 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Pseudomonas aeruginosa UPM P3 from Oil Palm RhizosphereBadri Fariman Azadeh, Sariah MeonProblem statement: Pseudomonas aeruginosa has been used in agriculture as biological agents. It has shown substantial control of a variety of soil-borne plant pathogens including Macrophomina phaseolina, Botrytis cinerea, Rhizoctonia solani, Colletotrichum truncatum, Pythium, Fusarium and others. Species aggregate of Pseudomonas aeruginosa strain UPM P3 was shown to have potential as a biocontrol agent against Ganoderma boninense, the causal agent … …American Journal of Applied Sciences, Vol. 6, Issue 11 · 30 Nov 2009 · 7,802 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetection of Phytoplasma on Indian Fig (Opuntia ficus-indica Mill) in Mexico Central RegionRicardo Hernández-Pérez, Juan Carlos Noa-Carrazana, Ricardo Gaspar et al.Problem statement: The Indian fig (a species of prickly pear cactus), has been known as Nopal, comprises an approximate area of 100,000 ha, in plantations used for human consumption. "Pyramids" Indian fig area located in the northeastern State of Mexico has been an important Indianfig area in the country, with 15810 ha, where a phytoplasma has been consistently present in … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,729 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssessment of Site Specific Mutational Effect on Transcription Initiation at Escherichia coli PromoterS. Kannan, M. Krishnan, S. Sundarraj et al.Problem statement: It is widely accepted thought that the weak promoters control the RNA synthesis and play regulatory role in complex genetic networks in bacterial system. An experiment had been designed to address whether mutations in the -16/-17 region affect the rate of transcription at an activator-independent promoter in E. coli or not? Approach: The aim of this study was … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 2 · 30 Jun 2009 · 7,555 accessesView PDFCite