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89 results for “Culture Medium”
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Research ArticleOpen accessIn the abstractIn Vitro Callus Induction and Regeneration of Medicinal Plant Datura innoxiaBaan Munim Twaij, Alaa Jabbar Taha, Md. Nazmul HasanThe present study indicate the possibility of the development of callus from stem cut, leaf and root explants and regeneration of Datura plant (Datura innoxia) on Murashige and Skoog (MS) media supplemented with different concentrations of 6-Benzylaminopurine (BA) and 1-Naphthaleneacetic (NAA). Sterilized explants were inoculated and incubated in culture shade under different conditions (light and dark). Data analysis showed that … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 3 · 17 Aug 2019 · 8,371 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Mother Plant and Somatic Embryo Registry for the Propagation and Conservation of Artificial Seeds of the Orchid Phragmipedium kovachiiOsber Cunia, Manuel Oliva, Segundo G. Chavez et al.Phragmipedium kovachii, is an endemic orchid that naturally inhabits the humid zones of the Amazonas region in Peru. Currently, it has been extracted from its natural habitat, listed as critically endangered by CITES, suggesting searching for conservation strategies in vitro and in situ. Therefore, this research established an in vitro protocol to obtain and conserve artificial seed, through four events: … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 8 Jul 2024 · 5,315 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Keratinolytic Activity Succeeds by Keratinophilic Fungi in Jaipur, IndiaVishnu Sharma, Anima Sharma, Ruchi SethEarth has innate background for fungi that cover individual kingdom since evolution. The keratinophilic fungi are allied moulds that produce the keratinase enzyme to degrade the keratinous materials in or on the soil. Keratinous materials are insoluble and resistant to degradation by common proteinase enzymes. It is important to study the microorganism producers of such enzymes for use in the … …American Journal of Applied Sciences, Vol. 14, Issue 7 · 12 Apr 2017 · 7,570 accessesView PDFCite
Research ArticleOpen accessIn the abstractChemical Mutagenesis for Improvement of Production of a Biologically Active Extracellular Polymeric Substance by Halomonas xianhensis SUR308Jhuma Biswas, Amal K. PaulA moderately halophilic bacterium, Halomonas xianhensis SUR308 (Genbank Accession No. KJ933394) isolated from solar saltern of Surala, Odisha, India was found to produce significant amount of a biologically active extracellular polymeric substances (EPS). Under optimized cultural conditions, the strain was able to produce 7.05 g L-1 of EPS after 8 days of incubation under shake culture. To enhance the EPS … …Current Research in Microbiology, Vol. 7, Issue 1 · 1 Apr 2016 · 15,926 accessesView PDFCite
Research ArticleOpen accessIn the abstractHide Dehairing and Laundry Detergent Compatibility Testing of Thermostable and Solvents Tolerant Alkaline Protease from Hot Spring Isolate Bacillus cohniiU3Pravin R. Dudhgara, Bhavsar Sunil, Ghelani AnjanaIn the present study, alkaline protease producing thermophilic Bacillus cohniiU3 strain was isolation from Unnai hot spring, India. The maximum production of protease (344 U/mL) was reported after 72 h of inoculation at 50°C in shake flask culture using the gelatin casein medium. The protease was found to remain active up to 96 h and production was growth dependent. The … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 23 Jul 2015 · 7,340 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation of Nitrate and Phosphate Removing Bacteria from Various Environmental SitesS. DebRoy, S. Das, S. Ghosh et al.Problem statement: Nitrate and phosphate are two major pollutants due to anthropogenic activity like excessive use of fertilizers in agriculture. Their contamination has emerged as a global problem and its potential threat is marked on the environmental sustainance as well as on the public health. Approach: The objective of the current study is to isolate efficient nitrate and phosphate removing … …OnLine Journal of Biological Sciences, Vol. 12, Issue 2 · 29 Jun 2012 · 9,354 accessesView PDFCite
Research ArticleOpen accessIn the abstractKEY PARAMETERS OF MEASLES VIRUS PRODUCTION FOR ONCOLYTIC VIROTHERAPYK. Weiss, D. Salzig, M. D. Mühlebach et al.Attenuated measles virus has revealed selective tumor cell killing and is currently tested in clinical trials for the therapy of cancer patients. The amount of infectious particles per dose needed for oncolytic therapy can be more than a million times higher compared to vaccination. This requires highly effective production processes which guarantee the measles virus quantities needed for its use … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 9,791 accessesView PDFCite
Research ArticleOpen accessIn the abstractUse of a Mixture of Thermophilic Enzymes Produced by the Fungus Thermoascus aurantiacus to Enhance the Enzymatic Hydrolysis of the Sugarcane Bagasse CelluloseJ. R. Monte, W. Carvalho, A. M. F. MilagresProblem statement: The production of hydrolytic enzymes by T. aurantiacus has been performed under solid-state fermentations using lignocellulosic materials. The influences of the inoculum size and of the fermentation medium on the production of hemicellulases and cellulases were studied. Filtrates from the cultures were used to hydrolyze a pulp of sugarcane bagasse and the produced enzymes were shown to be … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 4 · 13 Dec 2010 · 9,215 accessesView PDFCite
Research ArticleOpen accessIn the abstractHydrocarbon Biodegrading Potentials of a Proteus vulgaris Strain Isolated from Fish SamplesPatience O. Olajide, L. B. OgbeifunA Proteus vulgaris bacterium SR-1 was isolated from a freshly killed fish sample collected close to the point of crude oil spill in the Niger Delta region, Nigeria. Problem statement: The application of native bacterial species in bioremediation processes has long been desired, because they would be cost effective and efficient in terms of acclimation time. The ability to isolate … …American Journal of Applied Sciences, Vol. 7, Issue 7 · 31 Jul 2010 · 8,280 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrevalence and Antibiotics Susceptibility of Uropathogens in Patients from a Rural Environment, TamilnaduMaripandi Arjunan, Ali A. Al-Salamah, M. AmuthanProblem statement: Urinary Tract Infections (UTIs) in human, rural environment cause significant morbidity due to insanitary condition, lack of knowledge of personal hygiene, lack of patient’s compliance and economic burden. In our study, the bacterial etiologies and the resistance patterns found in human with serious UTIs and selecting optimal antimicrobial therapy. Approach: A total of 105 patients first morning mid … …American Journal of Infectious Diseases, Vol. 6, Issue 2 · 30 Jun 2010 · 8,712 accessesView PDFCite