87 results for “Cell Culture”

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Research ArticleOpen accessIn the abstractProgressive LDL Reduction to Very Low Levels Improves Dimeric Nitric Oxide Synthase, Nitric Oxide Bioavailability and Reduces Peroxynitrite in Endothelial Cells during HyperglycemiaR. Preston Mason, Hazem Dawoud, Samuel C.R. Sherratt et al.Circulating levels of glucose and LDL influence Endothelial Cell (EC) function in a highly interactive manner as evidenced by nitric oxide production and coupling of endothelial Nitric Oxide Synthase (eNOS) dimer. Here, we report on the status of eNOS function in Human Umbilical Vein Endothelial Cells (HUVECs) cultured under normo- and hyperglycemic (250 mg/dL) condition followed by exposure to increasing … …American Journal of Pharmacology and Toxicology, Vol. 14, Issue 1 · 2 May 2019 · 8,356 accessesView PDF Cite

Research ArticleOpen accessIn the abstractGenetic Diversity in Merozoite Surface Protein.1 of Plasmodium falciparum in Highlands: Wamena Papua IndonesiaRosye Hefmi Rechnelty Tanjung, Yulius Sarungu, Meidy Johana Imbiri et al.Malaria is the first killer as well as endemic disease in highland, lowland and coastal areas of Papua. In 2016 there were 160 thousands cases noted and 80% of the cases were caused by Plasmodium falciparum. Total sample for this study were provided by a clinical trial conducted from May to June 2018 in the regional hospital of Puncak Jaya, … …American Journal of Infectious Diseases, Vol. 14, Issue 4 · 11 Jan 2019 · 7,753 accessesView PDF Cite

Research ArticleOpen accessIn the abstractModeling Hepatocellular Toxicity of Enavatuzumab, a Humanized Anti-TweakR AntibodyDonghee Choi, Yanhong Zhu, Debra Tien Chao et al.In a Phase 1 clinical study, treatment with enavatuzumab, a humanized monoclonal antibody to TweakR, resulted in liver toxicity in a subset of patients. The objective of this current study was to evaluate the ability of preclinical studies to predict liver toxicity in humans. Enavatuzumab was evaluated in cynomolgus monkeys, where serum liver enzyme and cytokine levels were measured and … …American Journal of Pharmacology and Toxicology, Vol. 12, Issue 2 · 9 Nov 2017 · 7,719 accessesView PDF Cite

Research ArticleOpen accessIn the abstractHybrid Ceramo-Polymeric Nanocomposite for Biomimetic Scaffolds Design and PreparationRaffaella Aversa, Relly Victoria V. Petrescu, Roberto Sorrentino et al.Biomimetics, biomechanics and tissue engineering are three multidisciplinary fields that have been contemplated in this research to attain the objective of improving prosthetic implants reliability. Since testing and mathematical methods are closely interlaced, a promising approach seemed to be the combination of in vitro and in vivo experiments with computer simulations (in silico). An innovative biomimetics and biomechanics approach and … …American Journal of Engineering and Applied Sciences, Vol. 9, Issue 4 · 30 Nov 2016 · 8,269 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPhysiological Active Substance Changes of Soybean Curd Residue Fermented by Preussia aemulans and its BioactivityYiting Li, Shili Meng, Min Shi, Zhenya ZhangA new species of fungus Preussia aemulans isolated from the Cordyceps sinensis fruiting body was cultured by soybean curd residue using solid-state fermentation method. The content of polysaccharide, amino acid, protein, phenolics and nucleoside were massively improved under the optimum conditions, including 10% adding dosage of glucose, 3% adding dosage of beef extract, 15 days of fermentation time. The crude … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 6 Jan 2016 · 7,453 accessesView PDF Cite

Research ArticleOpen accessIn the abstractOPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORMNayera A.M. Abdelwahed, Enas N. Danial, Noura El-Ahmady El-Naggar, Asem A. MohamedOptimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL-1 were 30°C at pH 8.5 and incubation time 96 h in free cell cultures using starch 20 g L-1 as carbon source and yeast extract 5 g L-1 … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Dec 2013 · 8,125 accessesView PDF Cite

Research ArticleOpen accessIn the abstractA Case of Refractory Salmonella Spondylodiscitis in an Immunocompetent Patient Treated Via an Extracavitary Approach, Corpectomy and Placement of Expandable CageKimon Bekelis, Oren Gottfried, Zyia L. Gokaslan et al.Problem statement: Salmonella spondylodiscitis is a rare condition that is more prevalent in patients with sickle cell disease or immunosuppression. However, it can also be found in immunocompetent patients. It usually responds well to proper antibiotics. However, surgery is rarely indicated when the infection is refractory to antibiotics, the spine is destabilized or there is an epidural component that is … …American Journal of Infectious Diseases, Vol. 6, Issue 2 · 30 Jun 2010 · 7,533 accessesView PDF Cite

Research ArticleOpen accessIn the abstractThe Widely Used Diagnostics "DNA Microarray"-A ReviewR. Manoj KumarProblem statement: DNA microarray technique is one of the latest advances in the field of molecular biology and medicine. It is a multiplex technique used in combination of bioinformatics and statistical data analysis. Since, 1995, the technique offers the possibility of conducting tens or hundreds of thousands of simultaneous hybridizations. Approach: This increased experimental efficiency permits high throughput and whole … …American Journal of Infectious Diseases, Vol. 5, Issue 3 · 30 Sep 2009 · 7,787 accessesView PDF Cite

Research ArticleOpen accessIn the abstractThe Flavonoid, Quercetin, Inhibits HIV-1 Infection in Normal Peripheral Blood Mononuclear CellsMadhavan P.N. Nair, Zainulabedin M. Saiyed, Nimisha H. Gandhi, C. N. RamchandProblem statement: Flavonoids and related polyphenols, have been known to possess cardioprotective, anti-tumor, anti-inflammatory, anti-allergic and anti-viral activities. Previous studies have shown that flavonoid, quercetin significantly downregulates expression of pro-inflammatory cytokines in cultured cells via modulation of NFκB and p38MAPK signaling pathways. In the current study, we hypothesize that quercetin exerts anti-HIV activity by differential modulation of pro-and anti-inflammatory cytokine … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 12,184 accessesView PDF Cite

Research ArticleOpen accessIn the abstractRapamycin Inhibits Cystogenesis by Cystic Epithelial Cells Derived from Human Autosomal Dominant Polycystic KidneysGerard Elberg, Teresa V. Lewis, Lijuan Chen, Martin A. TurmanAutosomal dominant polycystic kidney disease (ADPKD) is one of the most common genetic disorders and carries a high health burden since it progresses to renal failure in half of the afflicted individuals by the 6th decade of life. Currently, there is no effective treatment to slow the progression of ADPKD. Rapamycin, a drug most commonly used as an immunosuppressive agent … …American Journal of Pharmacology and Toxicology, Vol. 1, Issue 4 · 31 Dec 2006 · 7,101 accessesView PDF Cite