Research ArticleOpen accessIn the abstractExtraction of Genomic DNA Using Magnetic Nanoparticles (Fe3O4) as a Solid-Phase SupportZ. M. Saiyed, C. N. RamchandMagnetic separation technology, using magnetic particles, is quick and easy method for sensitive and reliable captures of specific proteins, genetic material and other biomolecules. The current paper describes a universal genomic DNA extraction method optimized in our laboratory using magnetic nanoparticles as a solid phase adsorbent. The yields of the isolated DNA with magnetic method were higher or equivalent to … …American Journal of Infectious Diseases, Vol. 3, Issue 4 · 31 Dec 2007 · 9,826 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmunodeficient Parameters in the HIV-1 Transgenic Rat ModelSulie L. Chang, Frank Ocasio, Jose A. BeltranRecently an HIV-1 transgenic (HIV-1Tg) rat model was created that carries a gag-pol-deleted HIV-1 genome under the control of the HIV-1 viral promoter. However, other viral proteins are expressed in most organs and tissues, and are found in the circulating blood. Since HIV-1 targets the immune system in humans, we examined two immunological parameters, leukocyte-endothelial adhesion (LEA) and inflammatory cytokine … …American Journal of Infectious Diseases, Vol. 3, Issue 4 · 31 Dec 2007 · 7,834 accessesView PDFCite
Research ArticleOpen accessIn the abstractNutritional control of HIV-AIDS-A case for SeleniumParisa Kalantari, Hema Vunta, Umamaheswari D. Palempalli et al.Selenium (Se) is a micronutrient that is incorporated into proteins. Some of the selenoenzymes such as GPXs and thioredoxin reductases exhibit glutathione peroxidase-dependent activities towards organic and inorganic peroxides. Thus, selenoenzymes play a pivotal role in mitigating oxidative stress caused by accumulation of peroxides. In this respect, selenoproteins may interfere with the transcription-driven by oxidative stress-dependent signaling mechanisms to influence … …American Journal of Infectious Diseases, Vol. 3, Issue 4 · 31 Dec 2007 · 7,344 accessesView PDFCite
Research ArticleOpen accessIn the abstractRedox Sensing and Cardiac Myocyte Loss: Some Recent InsightsShyamal K. GoswamiGeneration of reactive oxygen-nitrogen species (ROS/RNS) has long been ascribed to pathophysiological conditions like ageing, neurodegenerative and cardiovascular disorders. However, more recent evidences suggest that while generation of ROS/RNS at a higher threshold is deleterious, at a lower dose these can act as second messengers, influencing specific cellular events like cell growth and differentiation, embryonic development, protection against ischemic injuries … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 4 · 31 Dec 2007 · 6,349 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of a DNA Vaccine for Immunocontraceptive Potential Against Zona Pellucida Glycoproteins in CattleC.A. Foley, S.M. Boyle, R.E. Pearson, F.C. GwazdauskasHolstein cows were administered zona pellucida (ZP) DNA vaccine and used to determine the potential of recombinant rabbit ZP glycoproteins (rZP) as immunocontraceptive antigens. Zona pellucida proteins were purified and quantified. Cows were assigned to one of four treatment groups in which plasmids encoding rabbit ZP proteins were administered, i.d., using a gene gun (ZP55, n=2; ZP75, n=2; Hep55, n=2; … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,888 accessesView PDFCite
Research ArticleOpen accessIn the abstractHigh Concentrations of Glucose can Activate or Inhibit Human Erythrocyte Aminolevulinate Dehydratase in vitro Depending Exposure TimeJ. C. Soares, D. Gabriel, V. Folmer et al.Hyperglycemia can cause oxidative stress and inactivate susceptible enzymes. In the present investigation we examined whether short-term incubations (24 or 48 h) with high concentrations of glucose (10-200 mmol L-1) inhibit ALA-D from human erythrocytes. Incubations of erythrocytes for 24 h with glucose (10 up to 40 mmol L-1) resulted in an increase of δ-ALA-D activity (P<0,001). Incubations of erythrocytes … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,686 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Mechanisms for Altered Glucose Homeostasis in HIV InfectionPaula W. HruzA complete understanding of the molecular mechanisms leading to HIV-associated insulin resistance remains elusive. Complex interrelationships between genetic predisposition, disease-related body changes and multidrug therapy all contribute to alterations in glucose homeostasis. These abnormalities can be differentiated between acute and reversible changes directly induced by HAART medications and more chronic and less reversible changes due to the development of lipodystrophy … …American Journal of Infectious Diseases, Vol. 2, Issue 3 · 30 Sep 2006 · 6,877 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Postgenomic Era: Transcriptomic as the New Paradigm of LifeBruno Giardina, Marcella Renis, Vito Latora, Salvatore MusumeciNow that complete genome sequences are available for a variety of organism, the elucidation of single gene function involved in the metabolism, includes necessarily a better understanding of the cellular responses on all levels of gene products, mRNA, proteins and metabolites. Such knowledge’s growth is essential since the observable properties of living organisms, so called phenotypes, are the results of … …OnLine Journal of Biological Sciences, Vol. 6, Issue 1 · 30 Jun 2006 · 6,822 accessesView PDFCite