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Research ArticleOpen accessIn the abstractOptical Properties of Crosslinked Chitosan Thin Film with Glutaraldehyde Using Surface Plasmon Resonance TechniqueYap Wing Fen, W. Mahmood Mat Yunus, Mohd Maarof Moksin et al.Problem statement: The aim of this research is to study the optical properties of crosslinked chitosan thin film with glutaraldehyde using Surface Plasmon Resonance (SPR) technique. Approach: The crosslinked chitosan is synthesized by homogeneous reaction of medium molecular weight chitosan in aqueous acetic acid solution with glutaraldehyde as crosslinking agent. Four different amount of chitosan, i.e., 0.40, 0.50, 0.60 and … …American Journal of Engineering and Applied Sciences, Vol. 4, Issue 1 · 28 Jan 2011 · 9,624 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Extractants on the Yields and Selected Chemical Characteristics of Humic Acids Isolated from Tropical Saprists PeatLee Jia Huey, Osumanu Haruna Ahmed, Nik Muhamad Ab. MajidProblem statement: Humic acids are beneficial to soil aggregation, binding of heavy metal, plant growth and many more. However, the isolation of Humic Acids (HA) from its origin is not only time-consuming, but the isolation is also affected by factors such as temperature and the types of extractants and their concentrations. Different concentrations of extractant are said to alter the … …American Journal of Applied Sciences, Vol. 7, Issue 7 · 31 Jul 2010 · 7,503 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of Salmonella enteritidis Outer Membrane Proteins and Lipopolysaccharide Profiles with the Detection of Immune Dominant ProteinsA. Maripandi, Ali A. Al-SalamahProblem statement: Salmonella infection is a serious medical and veterinary problem world-wide and causes great concern in the human health and food safety. Salmonella enteritidis outer membrane proteins and lipopolysaccharide play important role in the virulence and immunological properties of bacteria. Prophylaxis of enterobacterial infection has proven difficult to achieve. Vaccination is an effective tool for the prevention of Salmonella … …American Journal of Immunology, Vol. 6, Issue 1 · 21 Jun 2010 · 9,147 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71Farouk Al-Quadan, Hazem Akel, Rasha NatshiProblem statement: This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL-1) was obtained at 100°C after 10 min of incubation. The enzyme was purified 24 fold … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,400 accessesView PDFCite
Research ArticleOpen accessIn the abstractConcentration and Physicochemical Properties of Chitosan Derivatives Determine the Induction of Defense Responses in Roots and Leaves of Tobacco (Nicotiana tabacum) PlantsAlejandro B. Falcón-Rodríguez, Juan C. Cabrera, Eduardo Ortega, Miguel Ángel Martínez-TéllezProblem statement: The chitosan derivatives promote diverse defensive responses in plants, which are affected by chitosan chemical features and concentration. Glucanase (EC 3.2.1.6), Phenylalanine Ammonia-Lyase (PAL, EC 4.3.1.5) and peroxidase (POD, EC 1.11.1.6) are key enzymes in tobacco defense responses. Thus, the aim of this study was to know the behavior of their enzymatic activity in leaves and roots of … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 3 · 30 Sep 2009 · 9,263 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Review on Physicochemical and Thermorheological Properties of Sago StarchA. Mohamed, B. Jamilah, K.A. Abbas et al.This study was a part of a research project aiming to investigate the texture characteristics of protein - starch interaction in fish based product keropok lekor. Accordingly, the current review study focused on some physicochemical (molecular weight, viscosity, chemical composition and swelling power) and thermorheological (gelatinization, retrogradation and viscoelsticity) characteristics of sago starch alone and in mixtures with other ingredients … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 4 · 31 Dec 2008 · 9,639 accessesView PDFCite
Research ArticleOpen accessIn the abstractPotential Photooxidation Pathways of Dicarboxylic Acids in Atmospheric DropletsL.M. Yang, L.E. Yu, M.B. RayLow molecular weight (LMW) dicarboxylic acids (DCAs) have received increasing attention because of their hygroscopic properties and capability of acting as cloud condensation nuclei (CCN). Based on the concentration profiles of intermediates identified during laboratory photooxidation of C7-C9 DCAs in a liquid-phase reaction system, chemometric analyses were conducted to examine the degradation pathways of DCAs for the formation of secondary … …American Journal of Environmental Sciences, Vol. 4, Issue 5 · 31 Oct 2008 · 6,497 accessesView PDFCite
Research ArticleOpen accessIn the abstractProteins polymorphism of some populations of Lygeum spartum L. in AlgeriaAbderrezak Djabeur, Meriem Kaid-Harche, Douadi KhelifiLygeum spartum L. represent a natural barrier in the Algerian high plateaus against the advances of sand and turning into a desert. This species has two levels of ploïdy (diploïd cytotype 2n = 16 and polyploidy cytotype 2n = 40). Protein profile of several populations of this species have inter and intra specific variability. The polymorphic bands observed in the … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 1 · 31 Mar 2008 · 8,093 accessesView PDFCite
Research ArticleOpen accessIn the abstractUse of Cross-linking to Assess Subunit Interaction of Recombinant Human Coproporphyrinogen OxidaseJason R. Stephenson, Nancy E. Thomas, Jon A. Friesen, Marjorie A. JonesTo provide further evidence for a dimeric form of coproporphyrinogen oxidase reported using the conventional hydrodynamic methods, bifunctional cross-linkers were incubated with purified, recombinant human coproporphyrinogen oxidase to determine subunit interaction in solution. The use of cross-linkers provides an effective way to demonstrate subunit association and allows for assessment of activity upon covalent cross-linking. Following incubation with selected cross-linkers, enzyme … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,594 accessesView PDFCite
Research ArticleOpen accessIn the abstractHydroxyl Radical-Scavenging Activity-Guided Extraction of Peptides from Oviductus ranaeJieyang Shen, Yihui Liu, Jiahao Xu et al.Oviductus Ranae (OR) is a traditional Chinese medicinal material that is listed in Chinese Pharmacopeia with proteins the most abundant ingredients. In present work, pepsin/trypsin-based stepwise hydrolysis was applied to extract Antioxidant Peptides from OR (APOR) using hydroxyl radical-scavenging capacity as evaluation index. After single-factor experiment, response surface method coupled with Box-Behnken design were used for optimizing process parameters and … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 4 · 18 Oct 2021 · 6,609 accessesView PDFCite