86 articles for “Cell Culture”

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Research ArticleOpen accessIn the abstractTHE IMMUNOMODULATORY EFFECTS OF PROBIOTIC BACTERIA ON PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) OF ALLERGIC PATIENTSSomaya M. El Sheikh, Mona A.M. Shalaby, Raghda A. Hafez et al.Allergic diseases represent major health burden. An allergic reaction is characterized by a disrupted T- helper 1â„T-helper 2 balance toward a preferential allergen specifically induced TH2 cytokine profile, causing allergic inflammation Probiotic bacteria have various benificial effects in many pathologic situation. Studies have shown that the bacteria present in the intestinal micro flora play a role in the TH1/TH2 balance … …American Journal of Immunology, Vol. 10, Issue 3 · 10 Sep 2014 · 8,273 accessesView PDF Cite

Research ArticleOpen accessIn the abstractLemon Pepper Fruit Extract (Zanthoxylum acanthopodium DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages In VitroYanti, Theodorus Eko Pramudito, Nerissa Nuriasari, Katarina JulianaProblem statement: Lemon pepper fruits (Zanthoxylum acanthopodium DC.; Rutaceae) have been used as a traditional source against stomach ache by Batak people in North Sumatera province, Indonesia. However, its scientific evidence for treatment of inflammatory disorders particularly gastritis has not been reported. Approach: Here, we investigated the inhibitory effects of Lemon Pepper Fruit Extract (LPFE) against inflammatory biomarkers by conducting … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 14 Feb 2012 · 9,581 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCondoms-As Sources of Extracellular Enzyme Producing MicrobesMadhusmita Mishra, Poulomi Nandy, Sarvani Debsarma et al.Problem statement: Condoms are widely used birth control measures which are made of natural latex or synthetic materials like polyurethane or polyisoprene as matrix with lubricants added to make them ready to use commodities. Absence of sterilization method along with presence of lubricant like petroleum jelly (that are applied on the outer surface), prompted the investigators to look for microbes … …Current Research in Microbiology, Vol. 1, Issue 2 · 31 May 2011 · 8,985 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEarly Diagnosis of Breast Cancer using Molecular, Biochemical and Pathological MarkersSalah El-Din El-Assal, Adel A. El-Tarras, Samir M. Abd-allaProblem statement: Laboratory diagnosis of breast cancer in most of the hospitals has traditionally been performed using cell culture and the direct hormone receptor assay, which are money and time consuming. Approach: This study was performed in order to direct the attention toward increasing the efficiency of early diagnosis in clinical laboratories at the western region of KSA and Egypt … …American Journal of Applied Sciences, Vol. 8, Issue 1 · 31 Jan 2011 · 8,056 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCerebrospinal Fluid (CSF) Ferritin for Differentiation of Aseptic and Bacterial Meningitis in AdultsZhinous Bayat Makoo, Nagmeh Ahadi, Akbar Hasani et al.Problem statement: Bacterial meningitis is one of the most important causes of mortality and morbidity. Rapid diagnosis of bacterial cause is very important and critical, because early antibiotic therapy prevents complications; different tests have been developed for rapid diagnosis of bacterial meningitis. One of them is CSF ferritin, which is evaluated in this study. Approach: During cross sectional study from … …American Journal of Infectious Diseases, Vol. 6, Issue 4 · 31 Dec 2010 · 8,046 accessesView PDF Cite

Research ArticleOpen accessIn the abstractChitinolytic Bacteria Isolated from Chili Rhizosphere: Chitinase Characterization and Its Application as Biocontrol for Whitefly (Bemisia tabaci Genn.)Nisa Rachmania Mubarik, Irni Mahagiani, Amaryllis Anindyaputri et al.Problem statement: Chitin, a common constituent of insect exoskeleton, could be hydrolyzed by chitinase. The research was conducted to screen chitinolytic rhizobacteria isolated from rhizosphere of chilli pepper and to determine their chitinase activity in degrading chitin of whitefly, Bemisia tabaci Genn. (Hemiptera: Aleyrodidae). Whitefly is recognized as an important pest on many crops including chilli pepper. Approach: Screening and … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 4 · 11 Nov 2010 · 18,538 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCirculating Granzymes are associated with Bloodstream Infection and Death in Febrile Medical PatientsAnnelies N. Tacx, Angela M. Wolbink, C. E. Hack, A.B. Johan GroeneveldPlasma levels of granzymes are specific markers for the activation of cytotoxic T lymphocytes and natural killer (NK) cells but the impact of activation of these cells on the course of bacterial infections is not well understood. We prospectively studied the implications of circulating granzyme A and B (GrA, GrB) levels in febrile, medical and hospitalized patients (n=284), during 3 … …American Journal of Infectious Diseases, Vol. 3, Issue 1 · 31 Mar 2007 · 6,736 accessesView PDF Cite

Research ArticleOpen accessIn the abstractApplications of Real-time Reverse Transcription Polymerase Chain Reaction in Clinical Virology Laboratories for the Diagnosis of Human DiseasesR. Manojkumar, V. MrudulaEarly diagnosis that prevents further expansion of the causative agent and thereby aids control and eradication of infectious agents is critical for countries trying to obtain a particular disease free status. In these diagnostics speed is paramount, including the assay’s applicability, sensitivity, specificity, cost effectiveness and patentability. Real-time Reverse Transcription PCR (rRT-PCR) has revolutionized the field of molecular biology and … …American Journal of Infectious Diseases, Vol. 2, Issue 4 · 31 Dec 2006 · 6,553 accessesView PDF Cite

Research ArticleOpen accessIn the abstractExpression of Exosome-Derived MicroRNAs miR-21 and miR-135 are Differentially Regulated Among Dental Pulp Stem CellsMatthew Hunsaker, Brandon Richards, Adelle Fuller et al.Dental Pulp Stem Cells (DPSCs) are a type of mesenchymal stem cell that has the potential to differentiate into various types of cells and tissue. Previous research has demonstrated that DPSCs can be easily accessed and isolated from both permanent and deciduous teeth, such as wisdom teeth or third molars, although there is less information available about the mechanisms and … …Current Research in Medicine, Vol. 11, Issue 1 · 10 Jan 2023 · 10,533 accessesView PDF Cite

Research ArticleOpen accessIn the abstractProgressive LDL Reduction to Very Low Levels Improves Dimeric Nitric Oxide Synthase, Nitric Oxide Bioavailability and Reduces Peroxynitrite in Endothelial Cells during HyperglycemiaR. Preston Mason, Hazem Dawoud, Samuel C.R. Sherratt et al.Circulating levels of glucose and LDL influence Endothelial Cell (EC) function in a highly interactive manner as evidenced by nitric oxide production and coupling of endothelial Nitric Oxide Synthase (eNOS) dimer. Here, we report on the status of eNOS function in Human Umbilical Vein Endothelial Cells (HUVECs) cultured under normo- and hyperglycemic (250 mg/dL) condition followed by exposure to increasing … …American Journal of Pharmacology and Toxicology, Vol. 14, Issue 1 · 2 May 2019 · 8,356 accessesView PDF Cite