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617 articles for “SEP”

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Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of Two New Recombinant Antagonists of Fibrinogen ReceptorJianbo Yang, Jia Yao, Kun Yang et al.The gene sequence of Decorsin which is extracted from a kind of North American leeches was synthesized. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the carboxyl terminal 27 amino acid residues of Decorsin(AnnV-D27), were constructed. And a 10 amino acids linker peptide of GGGGSGGGGS was inserted between Annexin V and Decorsin in AnnV-D39. Using pET-28(a+) … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,918 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Investigation of the Relationship among Analyst Following, Managerial Ownership and Firm Valuation: From the Perspective of Agency TheoryI. J. ChenIn this study, the relationship among analyst following, managerial ownership and firm valuation is investigated from the perspective of agency theory by using a nonlinear simultaneous equation model. The data from the Taiwan stock market verify the proposed hypotheses with a nonlinear three-stage least-square procedure. The empirical results show that managerial ownership (serving an internal monitoring function) and number of … …Journal of Social Sciences, Vol. 1, Issue 1 · 31 Mar 2005 · 7,039 accessesView PDFCite
Research ArticleOpen accessIn the abstractChord Length Distributions of the HemisphereWilfried GilleThe distribution laws for two types of isotropic uniform random chords of the hemisphere, cap-chords and basic-chords, are investigated separately. From both distribution densities, the chord length distribution density of the whole hemisphere is derived. …Journal of Mathematics and Statistics, Vol. 1, Issue 1 · 31 Mar 2005 · 6,364 accessesView PDFCite
Research ArticleOpen accessIn the abstractAndrogen Receptor in Macrophages of Male Rat is Greater Than in FemaleKazem Ahmadi, Alan B. McCrudenThe presence and possible sex differences of androgen receptor in peritoneal macrophages was investigated using immunomagnetic beads. Macrophages were incubated with different concentrations of [3H]-5αDHT in the presence or absence of a 100 fold excess of unlabelled 5α-DHT. Labelled cells were separated from unbound steroid by immunomagnetic beads coated with anti-rat macrophage antibody. The binding identified in the rat macrophages … …American Journal of Immunology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,834 accessesView PDFCite
Research ArticleOpen accessIn the abstractMethyltert-butyl Ether (MTBE) Degradation by a Microbial ConsortiumS. B. Mortazavi, A. Nikpey, A. Rezaee et al.Methyl tert-butyl ether (MTBE) is added to reformulated gasoline to meet the 1990 Clean Air Act directives. Widespread use of MTBE in gasoline has resulted in groundwater contamination. Because of its undesirable effects on drinking water and ecologically harmful effects, MTBE removal has become a public health and environmental concern. In this study, we have isolated a mixed bacterial culture … …American Journal of Environmental Sciences, Vol. 1, Issue 1 · 31 Mar 2005 · 7,004 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled AdsorbentsQing Yang, Xuyu Lei, Jianqiang Xu, Lijia AnThe gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point of view of high-level and soluble expression, we herein constructed a recombinant gloshedobin without His-tag and developed a novel egg yolk antibody (IgY)-immunoaffinity chromatography for its purification in a … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,694 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Development of a New, Rapid, Amperometric Immunosensor for the Detection of Low Concentrations of Bacteria Part II: Optimization of the System for Escherichia coliEric Carnes, Ebtisam WilkinsThe optimization of a flow-through immunoassay system was conducted to achieve rapid detection of low concentrations of E. coli bacteria. The system includes a new immunosensor that uses newly developed, disposable immunofiltration membranes to yield a system that is sensitive as well as simple. Comparison of the performance of this system with conventional immunoassays demonstrated a significantly shorter time for … …American Journal of Applied Sciences, Vol. 2, Issue 3 · 31 Mar 2005 · 6,783 accessesView PDFCite