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130 results for “enzyme activity”
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Research ArticleOpen accessIn the abstractScreening of Yeast Strains for Pectinolytic Activity: Effects of Different Carbon and Nitrogen Sources in Submerged FermentationsMustafa Oskay, Hüsniye Tansel YalçinTwenty yeast strains were screened for pectinolytic activity, among the strains, only nine were positive for pectinase production. The best strain was Kluyveromyces marxianus NRRL-Y-1109 which gave high quantities of pectinase activity, by submerged fermentation. Different parameters such as incubation time, pH (3.5-7), temperature (20-45°C), nitrogen and carbon source, were optimized. The optimal incubation time, temperature and pH for pectinase … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 22 Jun 2015 · 7,818 accessesView PDFCite
Research ArticleOpen accessIn the abstractHeterologous Expression and Characterization of Thermostable Levansucrase (BsSacB) from Bacillus subtilis BB03Viniti Vaidya, Gajjeraman Prabu, D. Theertha PrasadThermostable levansucrase encoding gene (sacB) with its promoter was isolated from Bacillus subtilis BB03. BsSacB is composed of 1419 bp, encoding 473 amino acid residues and with promoter regions TTGCAA (-35) and TAGAAT (-10). The amino acid sequence analysis of BsSacB showed conserved motifs of microbial fructosyltransferase belonging to Glycoside Hydrolase family 68 (GH68). Comparative analysis of the protein structure … …OnLine Journal of Biological Sciences, Vol. 15, Issue 1 · 25 Mar 2015 · 7,376 accessesView PDFCite
EditorialOpen accessIn the abstractNeutrophil Extracellular Traps as Therapeutic Targets for Inflammatory DiseaseRory R. KoenenNeutrophilic granulocytes, or neutrophils are the most abundant circulating white blood cell and constitute a first line of defense against pathogens. They are characterized by a large polymorphic lobular nucleus and the presence of many secretory granules in their cytoplasm. Neutrophils are essential for a functional host defense, as is evidenced by the occurrence of severe infections in individuals with … …American Journal of Pharmacology and Toxicology, Vol. 9, Issue 4 · 25 Feb 2015 · 8,740 accessesView PDFCite
Research ArticleOpen accessIn the abstractTESTING CARBOXYMETHYL CELLULASE ACTIVITY SECRETED BY TRICHOPHYTON TERRESTRE IN CARBOXYMETHYL CELLULOSE SOLUTIONMansoor Ahmad Lone, Sanjay Sahay, Ravinder, DeepakThe techniques employed for bioconversion of cellulosic biomass to simple sugars by cellulases have a great industrial brunt. Cold-adapted microorganisms are potential resource of cold-active carboxymethyl cellulases secluded from the cold regions. In this study, Trichophyton terrestre-a rare fungal species in Indian soils, isolated from the rhizosphere of Juglans regia L. during winter season was subjugated for the production and … …Current Research in Microbiology, Vol. 5, Issue 1 · 4 Feb 2015 · 7,680 accessesView PDFCite
Research ArticleOpen accessIn the abstractProbing the Substrate Specificity and Intersubunit Interactions of Brevundimonas Diminuta Glutaryl Acylase with Site-Directed MutagenesisMichael Eldarov, Anna Lyashenko, Tatyana Sherbakova et al.Glutaryl-7-ACA acylases (GLA) are industrial enzymes widely used for the production of 7-aminocepahlosporanic acid (7-ACA)-the starting compound for manufacturing of semisynthetic cephalosporin antibiotics. Generation of mutant GLA's with increased activity and stability, capability for single-step conversion of cephalosporin C (CPC) directly to 7-ACA is a promising route to improve the current biocatalytic technologies. In this study GLA from Brevundimonas diminuta … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 3 · 12 Nov 2014 · 8,164 accessesView PDFCite
Research ArticleOpen accessIn the abstractINTERVENTION OF PHARMACOGENETIC PROFILING IN THERAPEUTICS OF KASHMIRI POPULATION, A LESSON LEARNED FROM CYP3A5*3 VARIANTAshaqullah Bhat, Aadil Wani, Rajinder Kaur et al.Human Cytochrome P450 3A, a variant of Cytochrome P-450- dependent monooxygenases is a major drug metabolizing enzyme. CYP3A has evolved to catalyze the biotransformation of its substrate. A great deal of inter-individual variation in expression levels exists for CYP3A5 gene. The common polymorphism in CYP3A5 that induces a cryptic splice site has been shown to cause a significant reduction in … …OnLine Journal of Biological Sciences, Vol. 14, Issue 3 · 10 Jul 2014 · 7,218 accessesView PDFCite
Research ArticleOpen accessIn the abstractASCORBIC ACID FOLIIAR SPRAY COUNTERACTING EFFECT OF SALINITY ON GROWTH, NUTRIENTS CONCENTRATIONS, PHOTOSYNTHESIS, ANTIOXIDANT ACTIVITIES AND LIPID PEROXIDATION OF BEAN (PHASEULUS VULGARIS L.) CULTIVARSZeinab A. Salama, El-Zanaty A.A. Abou El-Nour, Mohamed M. El Fouly, Alaa A. GaafarA water culture experiment was carried out in the Department of Fertilization Technology at National Research Centre, Cairo, Egypt, to investigate the effect of Ascorbic Acid (AsA) foliar application and salinity stress (100 ppm and 100 mmoL NaCl) on growth, nutrients concentration and some biochemical parameters of two kidney bean (Phaseulus Vulgaris L.) cultivars. Salinity caused significant reduction in growth … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 16 May 2014 · 7,906 accessesView PDFCite
Research ArticleOpen accessIn the abstractVARIABILITY IN THE LIGNINOLYTIC ENZYMES ACTIVITY BY LENTINULA EDODES IN SUBMERGED CULTURE WITH LIGNIN AND GLUCOSECitlalli Harris-Valle, Elisa Valenzuela-Soto, Alfonso Sánchez et al.The white-rot basidiomycete Lentinula edodes is the second most edible mushroom cultivated on the world. This fungus decomposes cell-wall associated macromolecules, is a natural degrader of lignin polymers. The differences in enzyme activities between strains of L. edodes provided useful information about the participation of enzymes in different development stages of the fungus. The effect of lignin on the fungal … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 28 Apr 2014 · 7,848 accessesView PDFCite
Research ArticleOpen accessIn the abstractEXERCISE TRAINING REVERSES THE DELETERIOUS EFFECT OF SUCROSE INTAKE ON INSULIN RESISTANCE AND VISCERAL FAT MASS DEPOSITION ON MICEJulio Cesar Mendes Soares, Vanderlei Folmer, Gustavo Puntel, João B.T. Da RochaAbnormal fat deposition has been identified as an important mechanism of obesity-associated with insulin resistance. Literature data also indicate that exercise can improve insulin action. The heme pathway enzyme δ-Aminolevulinate Dehydratase (δ-ALA-D) is a good marker for oxidative stress and this sulfhydryl enzyme is inhibited in such oxidative pathologies diabetes. The aim of this study was to evaluate the effects … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Mar 2014 · 7,324 accessesView PDFCite
Research ArticleOpen accessIn the abstractCELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOSNicole Mika, Katharina Cierpka, Michaela C. Lange et al.As requirements for Advanced Therapy Medicinal Product (ATMP) production differ from other production processes (e.g., therapeutic protein production), cell detachment is often a crucial step for the process success. In most cases, cell detachment is done enzymatically. Although many peptidases are established in cell culture in R&D, e.g., Trypsin as gold standard, many of them seem to be unsuitable in … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 8 Feb 2014 · 8,159 accessesView PDFCite