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210 results for “Rf Lines”
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Research ArticleOpen accessIn the abstractSequence Analysis of Scaffold/Matrix Attachment Regions (S/MARs) From Human Embryonic Kidney and Chinese Hamster Ovary CellsNur Shazwani Mohd Pilus, Azrin Ahmad, Nurul Yuziana Mohd YusofBinding of intergenic Scaffold/Matrix Attachment Regions (S/MARs) to nuclear matrix proteins is believed to poise adjacent genes for transcription by forming chromatin loops. Vector constructs containing Scaffold/Matrix Attachment Regions (S/MAR) flanking the gene of interest, therefore, are able to enhance recombinant protein expression in mammalian cells. We compared two methods that are based on buffers containing 2M NaCl and Lithium-3,5-diidosalicylate … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 8 Oct 2018 · 7,637 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Action of Bovine Cartilage on Tumor Cells In Vitro And In VivoArax Tanelian, Alexander M. AbdelnoorIn an earlier study we had investigated the effect of Bovine Cartilage (BC) on mouse melanoma cells. In pursuant to this study BC anti-tumor activity in vitro against several human tumor cell lines was evaluated, mechanism by which BC induces tumor cell death was studied and its immunogenicity was assessed. Five mice received Intraperitoneal (IP) injection of BC once every … …American Journal of Immunology, Vol. 14, Issue 1 · 23 Jan 2018 · 9,667 accessesView PDFCite
Research ArticleOpen accessIn the abstractNew Spring Wheat Mutation Resources with Improved Grain Quality, Metals Bioavailability and Yield ComponentsSaule Kenzhebayeva, Alfia Abekova, Guoping Zhang et al.Genetic variability in micronutrients is limited in wheat varieties. To broaden variation, seeds of cv. Eritrospermum-35 were irradiated with 100 and 200 Gy using 60Co, the treated seeds were grown to M7 generation with successive selection of the highest yield lines in each generation. Selected lines were evaluated for yield and grain quality traits such as iron, zinc and phytic … …American Journal of Agricultural and Biological Sciences, Vol. 12, Issue 4 · 2 Jan 2018 · 8,676 accessesView PDFCite
Research ArticleOpen accessIn the abstractSignal Pathway Regulation in Mammalian Cells in Wireless Communication EnvironmentShengdong LiTo regulate the signal pathway in mammalian cells in a wireless communication environment, an LED light stimulation system has been designed and constructed. To avoid interference from too many data lines, a wireless communication mode has been employed. In this study, HeLa cells and Drosophila melanogaster cells are used as the research objects. The CRY2/CIBN light genetic element system is … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 24 Nov 2025 · 2,003 accessesView PDFCite
Research ArticleOpen accessIn the abstractAn Investigation of Mold Cavity Pressure of Self-Clamping Molds During Solid Tire ManufacturingSadeep Amantha Mawathage, Jayashingha Arachchige Indika Sanjeewa, Nirosh Dilruk JayaweeraThis study investigates the mold cavity pressure in self-clamping molds used for solid tire manufacturing, a sector where Sri Lanka is a leading global supplier. Our research presents a detailed investigation into the mold cavity pressure of self-clamping molds used in solid tire production. By integrating experimental data, the effects of critical parameters such as clamp bolt torque, final tire … …American Journal of Engineering and Applied Sciences, Vol. 18, Issue 1 · 2 Oct 2025 · 2,971 accessesView PDFCite
Research ArticleOpen accessIn the abstractStabilization of Non-Surgical Polytrauma in Animal LimbsMarcelo Adrián Santamaría Brunengo, Santiago Ferrándiz BouThis project aims to identify and solve, or at least alleviate, certain problems associated with limb injuries in the veterinary area, where circumstances that are common in the medical sector have simple solutions but in animals represent long, painful processes and in a very large number of cases death, events that are easily solvable but which, due to a lack … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 1 · 21 Apr 2025 · 4,708 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluating the Impact of Topography on Flooding in Owerri North, Imo State, Nigeria Using GIS and Remote Sensing TechniquesSylvanus Ibeabuchi IroThe aim of this study is to analyze the topographical contribution to floods in Owerri, North Imo State, Nigeria. Research was carried out to ascertain whether topographical elements played a role in the flooding in specific areas of Owerri North. As a result, very little research has looked at the effects of topography on flooding. Thus, there is a barrier … …American Journal of Environmental Sciences, Vol. 21, Issue 1 · 29 Jan 2025 · 7,208 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Investigation of Organelle Genomes of Extra Nuclear Sunflower Mutants with Variegated PhenotypeIrina Dremuk, Nikolay Kozel, Mariya Logacheva et al.The comparative analysis of complete chloroplast and mitochondrial DNA sequences of the original inbred line 3629 and three extra nuclear mutant lines variegated-1, variegated-15, variegated-17, obtained by the N-nitroso-N-methylurea induced mutagenesis, was done. The studied mutant lines were presented two different phenotypes with diverse chlorophyll content: Pale/green mutant line variegated-17 with 1.8% relative (to 3629 line) chlorophyll content in mutant … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 24 Oct 2017 · 7,638 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioactive Compounds from Sponge Associated Bacteria: Anticancer Activity and NRPS-PKS Gene Expression in Different Carbon SourcesJepri Agung Priyanto, Rika Indri Astuti, Jun Nomura, Aris Tri WahyudiSponge associated bacteria with diverse NRPS-PKS genes could be the alternative source of bioactive compounds. This study aimed to analyze the activity as well as the regulatory mechanism regarding the anticancer properties derived from these bacteria against cancer cell lines. Extraction of bacterial metabolites using ethyl acetate solvent resulted yield ranging from 0.003 to 0.016% (w/v). Six extracts of bacterial … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 5 Oct 2017 · 8,891 accessesView PDFCite
Research ArticleOpen accessIn the abstractGIS-Based Optimal Site Selection for Installation of Large-Scale Smart Grid-Connected Photovoltaic (PV) Power Plants in Selangor, MalaysiaSabo Mahmoud Lurwan, Mohammed Oludare Idrees, Goma Bedawi Ahmed et al.This study presents a GIS-based model to identify optimal sites to install large-scale smart grid-connected Photovoltaic (PV) power plants. Input datasets include digital elevation model, road networks, grid lines and daily average solar radiation. Using multi-criteria decision-making approach, we set constraining conditions for slope, proximity to the road, proximity to grid lines, solar radiation and land use to optimize the … …American Journal of Applied Sciences, Vol. 14, Issue 1 · 30 Jan 2017 · 8,793 accessesView PDFCite