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109 articles for “DNA analysis”
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Research ArticleOpen accessIn the abstractThe Porcine Mitochondrial Transcription Factor a Gene: Molecular Characterization, Radiation Hybrid Mapping and Genetic Diversity among 12 Pig BreedsTanja Kunej, Xiao-Lin Wu, Jennifer J. Michal et al.Problem statement: Mitochondrial transcription factor A (TFAM) is a nucleus-encoded protein that is a key activator of mitochondrial transcription as well as a major participant in mitochondrial genome replication. Genomic characterization of the porcine TFAM gene is, therefore, necessary to determine its involvement in regulation of fat depots and meat quality traits in pigs. Approach: Genomic DNA sequence was determined … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 4 · 31 Dec 2009 · 6,870 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Widely Used Diagnostics "DNA Microarray"-A ReviewR. Manoj KumarProblem statement: DNA microarray technique is one of the latest advances in the field of molecular biology and medicine. It is a multiplex technique used in combination of bioinformatics and statistical data analysis. Since, 1995, the technique offers the possibility of conducting tens or hundreds of thousands of simultaneous hybridizations. Approach: This increased experimental efficiency permits high throughput and whole … …American Journal of Infectious Diseases, Vol. 5, Issue 3 · 30 Sep 2009 · 7,787 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity Analysis of the Gohilwari Breed of Indian Goat (Capra hircus) Using Microsatellite MarkersSubodh Kumar, S. P. Dixit, N. K. Verma et al.Problem statement: Gohilwari breed of goat is a multipurpose goat mainly for milk and meat purposes and best suited in its harsh climatic condition. This breed is inadequately characterized till now at DNA level. So the present study was undertaken for population genetic analysis at molecular level to exploit the breed for planning sustainable improvement, conservation and utilization, which subsequently … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 3 · 30 Sep 2009 · 8,925 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Opium Poppy (Papaver somniferum) GermplasmHari Shanker Acharya, Vimal SharmaProblem statement: The medicinal value of opium poppy (2n = 22, Papaver somniferum L.) is due to presence of more than two dozen alkaloids. The genetic origin and diversity of cultivars used in the breeding collection is not fully known. To evaluate the genetic diversity of the 24 germplasm currently cultivated in the state to provide genetic information about heterogeneity, … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,096 accessesView PDFCite
Research ArticleOpen accessIn the abstractRadiation Induced DNA Double Strand Break Studies of a Metal Sensitive Novel Bacterial Isolate from East Calcutta WetlandSanhita Chowdhury, Anindita Chakraborty, Ashoke Ranjan Thakur, Shaon Ray ChaudhuriProblem statement: This study was an attempt to isolate anaerobic microbes with potential for DNA double strand break repair using methanogen specific medium (DSMZ 120) from East Calcutta Wetland in India. It also intended to verify the specificity of the medium for isolation of the desired family of microbe. Approach: Culture based technique was used to obtain the pure isolate … …American Journal of Environmental Sciences, Vol. 5, Issue 3 · 30 Jun 2009 · 7,123 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity of Red Chittagong Cattle Using Randomly Amplified Polymorphic DNA MarkersMohammad Masud Rana Mufti, Mst. Parvin Mostari, Gautam Kumar Deb et al.Problem statement: Red Chittagong Cattle (RCC) is a potential genetic resource in Bangladesh having dairy potentialities, high resistance to disease and survive harse environmental condition while require low input supply. This resource is now disappearing due to indiscriminate crossing with other local and exotic cattle. To conserve this resource, find out the genetic diversity of RCC is prime concern. Approach: … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 1 · 17 Mar 2009 · 8,680 accessesView PDFCite
Research ArticleOpen accessIn the abstractHistological Features and ki-67 Index In Cervical Atypical LesionsD. Cabibi, L. Giovannelli, M. Cacciatore et al.One of the most frequent challenges in routine diagnostic assessment of cervical biopsies consists of some lesions in which histological features of HPV infection are either inconsistently present or only mildly/focally evident. We named them Atypical Lesions (AL) because differential diagnosis with metaplastic/reactive lesions and LSIL is difficult. It would be important to identify among them those lesions that are … …American Journal of Infectious Diseases, Vol. 4, Issue 3 · 30 Sep 2008 · 7,231 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Identification of Listeria monocytogenes in Processed Meat by a Combined Cultural-molecular MethodAngela Ingianni, Marta Quartuccio, Maria Antonietta Madeddu et al.The isolation and identification of Listeria monocytogenes in processed meat samples by a combined cultural-molecular method is described. It allows the identification of Listeria strains by means of a hybridization technique with a specific DNA probe directed to the listerial internalin gene. The specificity of this method was found to be 100% and sensitivity was as low as 1 CFU/2.5 … …American Journal of Infectious Diseases, Vol. 3, Issue 3 · 30 Sep 2007 · 7,252 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Black Bengal and Jamuna Pari Goat Breeds By RAPD MarkersM.A. Rahman, S.M.M. Rahman, M.A. Jalil et al.This experiment was conducted in the Genetics, Breeding and Reproductive Biotechnology Laboratory under Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute (BLRI), Saver, Dhaka, Bangladesh. DNA was extracted from 14 goat breeds and the extracted DNA was observed by gel electrophoresis. Eight goat specific primers were synthesized by ASM-800 DNA synthesizer and screened in the study and all … …American Journal of Animal and Veterinary Sciences, Vol. 1, Issue 2 · 31 Dec 2006 · 9,584 accessesView PDFCite
Research ArticleOpen accessIn the abstractUltrastructural and Immunological Characterization of Hepatitis C Core Protein-DNA Plasmid ComplexesNelson Acosta-Rivero, Yaraima Aguilera, Viviana Falcon et al.Recently, it has been shown that a truncated HCV core (HCcAg) variant, covering the first 120 aa (HCcAg.120), interacts with plasmid DNA vaccine (pIDKE2), encoding the HCV structural proteins (HCcAg, E1 and E2). In the present work, HCcAg.120-pIDKE2 complexes, forming heterogeneous packaged structures, were visualized using a negative stain/rotary shadow technique. Interestingly, 72 hours after intramuscular injection of HCcAg.120-pIDKE2 complexes … …American Journal of Immunology, Vol. 2, Issue 3 · 30 Sep 2006 · 6,743 accessesView PDFCite