53 results for “Affinity”

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Research ArticleOpen accessIn the abstractGeochemical Distribution of Trace Metals and Assessment of Anthropogenic Pollution in Sediments of Old Nakagawa River, Tokyo, JapanH.M. Zakir, Naotatsu Shikazono, Kazuo OtomoThe geochemical distributions of cadmium (Cd), copper (Cu), chromium (Cr), lead (Pb) and zinc (Zn) were examined in sediments collected from Old Nakagawa River (NR), Tokyo, Japan. A widely used 5-step sequential extraction procedure was employed for the fractionation of the metals and the concentrations were measured in the liquid extracts by inductively coupled plasma mass spectrometry (ICP-MS). The association … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,453 accessesView PDF Cite

Research ArticleOpen accessIn the abstractBiosorption of Cr(III) by Microalgae and Macroalgae: Equilibrium of the ProcessIzabela Michalak, Agnieszka Zielinska, Katarzyna Chojnacka, Jan MatulaEquilibrium experiments of chromium(III) biosorption by microalga (Spirulina sp.) and macroalga (Pithophora varia) were investigated in this paper. Influence of the following process parameters was studied: pH, biomass concentration and temperature. The equilibrium of the process was described with Langmuir equation. The model parameters were determined at various process conditions. The best operation conditions for the process were selected: pH … …American Journal of Agricultural and Biological Sciences, Vol. 2, Issue 4 · 31 Dec 2007 · 8,204 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPurification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled AdsorbentsQing Yang, Xuyu Lei, Jianqiang Xu, Lijia AnThe gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point of view of high-level and soluble expression, we herein constructed a recombinant gloshedobin without His-tag and developed a novel egg yolk antibody (IgY)-immunoaffinity chromatography for its purification in a … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,690 accessesView PDF Cite