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513 results for “Hybrid”
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Research ArticleOpen accessIn the abstractEvidences of Hepatitis C Virus Replication in Hepatocytes and Peripheral Blood Monocuclear Cells from Patients Negative for Viral RNA in SerumViviana Falcón, Nelson Acosta-Rivero, Mineko Shibayama et al.In this study, we examined the expression of hepatitis C virus (HCV) components in hepatocytes and peripheral blood mononuclear cells (PBMC) from patients positive for anti-HCV antibodies and negative for serum HCV-RNA. The samples obtained from 25 randomly selected patients (13 of 25 patients showed no histological evidences of chronic hepatitis and had normal serum ALAT and GGTP levels) were … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 7,164 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Transcript Labeling Techniques and Development of a Membrane-based Parallel Gene Expression AssayMark A. Fisher, Bonnie B. Plikaytis, Thomas M. ShinnickAn inexpensive alternative to micro arrays was developed to examine the expression of small sets of genes in parallel and used to compare direct and indirect transcript labeling methods. Psoralen-biotin direct labeling was ~10-fold less sensitive than reverse transcriptase-biotin-dUTP labeling, but the enzymatic method generated higher levels of background noise in miniarray hybridizations. The reproducibility of hybridization intensities was the … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,868 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Banana (AA) Diploids with Contrasting Levels of Black and Yellow Sigatoka ResistanceClaudia Fortes Ferreira, Sebastiao Oliveira Silva, Nivia Paula Damasceno Sobrinho et al.Most banana cultivars are susceptible to many diseases, whereas Sigatoka leads to greatest yield losses. One of the strategies to overcome this disease is thorough banana genetic breeding which consists in the obtainment of improved (AA) diploids which are then crossed with triploids obtaining (AAAB) tetraploid disease resistant bananas also presenting other important agronomic characteristics. The prior knowledge of the … …American Journal of Applied Sciences, Vol. 1, Issue 4 · 31 Dec 2004 · 7,652 accessesView PDFCite