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85 results for “purification”
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Research ArticleOpen accessIn the abstractBiotransformation Using Recombinant Cmp Sialic Acid Synthetase and α-2, 6-Sialyltran Sferase: Enzymatic Synthesis of SialosidesUlrike Hubl, Cynthia Sun, Shuguang Zhang et al.In this research, we successfully expressed recombinant CMP-sialic Acid Synthetase (CSS) from Neisseria meningitides and 2,6-Sialyltransferase (SAT) from Photobacterium damsela in E. coli BL21(DE3) fermented at a scale of up to 8 litres using individual plasmids pIRL-1 and pIRL-4b, respectively. After cell lysis with BugBuster, enzyme levels of 2U and 22U per litre were produced for CSS and SAT, respectively. … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 8,854 accessesView PDFCite
Research ArticleOpen accessIn the abstractEast Kolkata Wetland: A Multifunctional Niche of International ImportanceI. Mukherjee, S. Ray Chaudhuri, D. Ghosh, A. R. ThakurProblem statement: In the megacity Kolkata, the need for space to accomodate the ever increasing population is increasing day by day. As a result the encoarchment of the large wetland on the eastern frindge of the city is increasing and so is the threat of its loosing the status of Ramsar Status. Approach: Proper documentation of the existing traditional practice … …OnLine Journal of Biological Sciences, Vol. 12, Issue 2 · 31 Jul 2012 · 9,082 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiochemical Studies on Recombinant Human Isobutyryl-CoA DehydrogenaseNasser E. Ibrahim, Al-Walid A. MohsenProblem statement: Human Isobutyryl-CoA Dehydrogenase (IBD) is member of the Acyl-CoA Dehydrogenases family. It is involved in Val metabolism. In this study we modified the purification method of the IBD and further spectroscopic characterization was conducted. Approach: Using DTT in the buffers during the purification, IBD purified to homogeneity by different chromatographic steps. Reactivity of Cys residues were measured using … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 2 · 12 Aug 2011 · 7,539 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioaugmentative Approaches for Dairy Wastewater TreatmentIrina Schneider, Yana TopalovaProblem statement: The achievement of a good ecological status of water receivers after discharge of waste or partially treated water from dairy industry requires harmonic interaction between water treatment technology and self-purification processes. Approach: The present research included two modules. First: an anaerobic treatment process for dairy wastewater in broadly spread sequencing batch bioreactor with fixed biomass was studied. As … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 4 · 13 Dec 2010 · 8,894 accessesView PDFCite
Research ArticleOpen accessIn the abstractReduction of Isolation Period of Coal Humic AcidsClaudia Hui Hui Chen, Osumanu Haruna Ahmed, Nik Muhamad Ab. Majid, Mohamadu Boyie JallohProblem statement: The isolation of Humic Acids (HA) from coal is laborious, costly and time consuming. The extraction and fractionation periods of HA vary from 4 h to 7 days. Fractionation period ranges from 12-24 h. However, most studies use 24 h as extraction period and also 24 h as fractionation period. This study was conducted to investigate whether the … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 7,234 accessesView PDFCite
Research ArticleOpen accessIn the abstractSimple and Rapid Method of Isolating Humic Acids from Tropical Peat Soils (Saprists)Shamsuddin Rosliza, Osumanu Haruna Ahmed, Kasim Susilawati et al.Problem Statement: The isolation (extraction, fractionation and purification) of humic acids (HA) from soils is laborious, time consuming and expensive. The extraction, fractionation and purification periods of these substances vary from 12 h-7 days. In order to facilitate production of HA at competitive cost, this study was conducted to investigate whether a simple and rapid procedure could be developed for … …American Journal of Applied Sciences, Vol. 6, Issue 5 · 31 May 2009 · 8,552 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetermination of Minimal Duration Essential for Isolation of Humic Acids From Soils in Forest Restoration ProgrammesMohd Ramzan Nur Hanisah, Osumanu Haruna Ahmed, Kasim Susilawati et al.This study was conducted to investigate whether a simple and rapid method could be developed for extracting, fractionating and purifying soil HA in forest rehabilitation programmes. Humic acids from 10 g of soil were extracted with 100 mL of 0.10 M NaOH. Different extraction periods (4, 8, 12, 16, 20 and 24 h) were tested. Samples were centrifuged (16,211 G … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 3 · 30 Sep 2008 · 8,173 accessesView PDFCite
Research ArticleOpen accessIn the abstractUnconventional Approach for Demineralization of Deproteinized Crustacean Shells for Chitin ProductionN.S. Mahmoud, A.E. Ghaly, F. ArabChitin is a versatile environmentally friendly modern material. It has a wide range of applications in areas such as water treatment, pulp and paper, biomedical devices and therapies, cosmetics, membrane technology and biotechnology and food applications. Crustacean waste is the most important chitin source for commercial use. Demineralization is an important step in the chitin purification process from crustacean waste. … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 1 · 31 Mar 2007 · 8,799 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction and Characterization of Hydrocarbon Degrading Peroxidase from Rhizopus and Saccharomyces SpeciesFerdinand U. Paschaline, Onyeocha O. Ignatius, Ezeji E. Uchechukwu et al.The production, characterization and partial purification of hydrocarbon degrading peroxidase from di-culture of Rhizopus and Saccharomyces species was assessed. The fungi were isolated from hydrocarbon polluted soil and identified using standard microbiological and biochemical techniques. Fermentation was carried out for enzyme production and the extracellular extract was precipitated with 50% ammonium sulphate salts and further purified by gel filtrationusing sephadex … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 7 Dec 2019 · 7,398 accessesView PDFCite