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51 articles for “Real-time PCR”
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Research ArticleOpen accessIn the abstractInfluence of Heirloom Tomato Polyphenol Extracts on Expression of Inflammation Genes in BovineKwaku Gyenai, Nona Mikiashvili, Hamid Ismail, Mulumebet WorkuThe effect of tomato on diseases of economic importance continues to be of broad interest. To evaluate these relationships, the effect of crude extracts of Cherokee purple tomato on pro-inflammatory genes and cytokines were evaluated. Neutrophils from three Holstein Fresian cows were treated with Phosphate Buffered Saline (PBS) or E. coli O111-B4 Lipopolysaccharide (LPS). Treated cells were exposed to different … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 3 · 25 Aug 2012 · 8,377 accessesView PDFCite
Research ArticleOpen accessIn the abstractStudy on Survival of Chlamydia trachomatis in the Presence of Antichlamydial DrugsApurb Rashmi Bhengraj, Pragya Srivastava, Harsh Vardhan et al.Problem statement: Recurrent genital Chlamydia trachomatis infections due to treatment failures may results in complex sequelae leading to reproductive complexity and morbidity. It can be resulted by the heterotypic resistance with decreased drug susceptibility characteristic of the isolate. Studies are needed to understand the treatment failures and resistance characteristic of C. trachomatis. Hence, in vitro study was conducted on C. … …American Journal of Infectious Diseases, Vol. 8, Issue 1 · 20 Feb 2012 · 7,768 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Niacin on Hyperleptinemia and Ob Gene mRNA Over-Expression in Adipose Tissue of Dexamethasone Treated RatsTahoora Shomali, Mahnaz Taherianfard, Mehdi Fazeli, Niloofar SafaeiProblem statement: Glucocorticoid-induced ob gene over-expression and resulted hyperleptinemia may lead to adverse consequences especially on cardiovascular system; therefore, the present study was conducted to evaluate the effects of niacin on hyperleptinemia and ob gene over-expression due to dexamethasone administration in rats. Approach: Twenty four adult male rats divided randomly into four equal groups: 1- normal saline (control), 2- dexamathasone … …American Journal of Pharmacology and Toxicology, Vol. 6, Issue 2 · 17 Aug 2011 · 7,988 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Induction of Lycopene in Germinating Cottonseed with 2-(4-Methylphenoxy) Triethylamine (MPTA)Lorraine S. Puckhaber, Jinggao LiuAbstract: Problem Statement: Increasing carotenoids content in foods was currently of great interest. The present paper reports strong stimulatory effect of MPTA on the carotenogenesis in cotton, which was used as a model. Approach: Cotton seedlings were treated with MPTA vs. water and germinated in the dark. The levels of carotenoids were determined by HPLC and the expression levels of … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 1 · 26 Jul 2011 · 7,950 accessesView PDFCite
Research ArticleOpen accessIn the abstractAdiponectin mRNA Expression in the Cat (Felis domesticus)Angela L. Lusby, Stephen A. Kania, Joseph W. Bartges, Claudia A. KirkProblem statement: Adiponectin is a hormone expressed from adipose tissue in people, rodents and dogs. Adiponectin has anti-inflammatory action with beneficial effects on cardiovascular health and insulin sensitivity. With increasing fat mass, adiponectin concentrations paradoxically decrease. Adiponectin’s role in metabolism and diabetes mellitus is of interest in feline medicine because cats are susceptible to developing type II diabetes with weight … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 4 · 27 Dec 2010 · 7,784 accessesView PDFCite
Research ArticleOpen accessIn the abstractMethamphetamine Regulation of Sulfotransferases in Rat Liver and BrainTianyan Zhou, Chaoqun Huang, Yue Chen et al.Problem statement: Sulfotransferases (SULTs) are important phase II drug metabolizing enzymes. They also regulate the biological activities of various hormones. To the best of our knowledge, psychostimulants regulation of SULTs is basically not studied except one article reported that Methamphetamine (METH) treatment induced rat amygdale rSULT1A1 mRNA 4.3 fold by using microarray method to identify a series of candidate genes … …American Journal of Pharmacology and Toxicology, Vol. 5, Issue 3 · 30 Sep 2010 · 7,683 accessesView PDFCite
Research ArticleOpen accessIn the abstractGhrelin Gene Expression in Broiler Proventriculus Tissue are Changed by Feed Restriction, Different Dietary Energy and Protein LevelsShokoufe Ghazanfari, Mohammad Reza Nassiry, Mojtaba Tahmoorespur et al.Problem statement: The aim of this study was to investigate the effects of feed restriction and different energy and protein contents of diet on ghrelin gene expression in broiler chicken. Approach: Feeding programs consisted of ad libitum and feed restriction, two energy levels (3100 and 2800 kcal ME kg-1) and three protein levels (22.3, 19.3 and 16.3% CP). Feed restriction … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 7,481 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Flavonoid, Quercetin, Inhibits HIV-1 Infection in Normal Peripheral Blood Mononuclear CellsMadhavan P.N. Nair, Zainulabedin M. Saiyed, Nimisha H. Gandhi, C. N. RamchandProblem statement: Flavonoids and related polyphenols, have been known to possess cardioprotective, anti-tumor, anti-inflammatory, anti-allergic and anti-viral activities. Previous studies have shown that flavonoid, quercetin significantly downregulates expression of pro-inflammatory cytokines in cultured cells via modulation of NFκB and p38MAPK signaling pathways. In the current study, we hypothesize that quercetin exerts anti-HIV activity by differential modulation of pro-and anti-inflammatory cytokine … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 12,184 accessesView PDFCite
Research ArticleOpen accessIn the abstractMicrobial Biodiversity Screening for Metal Accumulators from Mineral Ore Rich Site in Andhra Pradesh, IndiaAditya Kumar Sarkar, Susmita Roy, Ananya Pal et al.Problem statement: Since the site selected for screening was a source of magnetite, the objective of this study was to isolate some groups of magnetotactic bacteria and microbes with potential of metal accumulation. Approach: The culture based approach was used for the study. Pure isolates obtained were characterized on the basis of morphology, biochemical tests, physiology as well as at … …OnLine Journal of Biological Sciences, Vol. 8, Issue 2 · 30 Jun 2008 · 7,546 accessesView PDFCite
Research ArticleOpen accessIn the abstractTherapeutic Effects of Phyllanthus Species: Induction of TNF-α-mediated Apoptosis in HepG2 Hepatocellular Carcinoma CellsSripathi M. Sureban, Dharmalingam Subramaniam, Paramasivam Rajendran et al.Phyllanthus species, especially P. amarus is a medicinal plant with hepatoprotective activity, believed to occur in part by inhibiting hepatitis B virus function. The pathogenesis mechanism of viral hepatitis is closely related to hepatocyte necrosis apoptosis. The aim of our study was to investigate the in vitro effects of extracts from three Phyllanthus species, P. urinaria, P. amarus and P. … …American Journal of Pharmacology and Toxicology, Vol. 1, Issue 4 · 31 Dec 2007 · 9,005 accessesView PDFCite