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61 articles for “PCR detection”
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Research ArticleOpen accessIn the abstractDetermination of the Presence of Huanglongbing in Seeds and Movement of the Pathogen in Citrus reticulataHajivand Shokrollah, Thohirah Lee Abdullah, Kamaruzaman Sijam, Siti Nor Akmar AbdullahProblem statement: Huanglongbing (HLB) also known as citrus greening disease is a fastidious phloem-inhabiting bacterium in the genus Candidatus Liberibacter. Using universal primers, the 16S ribosomal DNA sequence of three strains of the bacterium were obtained by PCR. However there is very little information on seed transmission and HLB pathogen movement to find a way for control or reduce the … …American Journal of Applied Sciences, Vol. 6, Issue 6 · 30 Jun 2009 · 8,088 accessesView PDFCite
Research ArticleOpen accessIn the abstractLack of Association of Glutathione S-transferase Gene Polymorphisms in Iranian Prostate Cancer SubjectsB. Sahar Ansari, R. Vasudevan, M. Mirinargesi et al.Problem statement: Glutathione S-transferase is super family genes that encode enzymes which involve in the detoxification of the cell and protects DNA from damage. Conflicting results was found in several studies in association with GSTT1 and GSTM1 gene polymorphisms and prostate cancer. In this study, we determined the association between the GSTT1 and GSTM1 gene polymorphism and prostate cancer in … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 1 · 31 Mar 2009 · 8,273 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetection of Chlamydophila abortus in Sheep (Ovis aries) in MexicoJuan Manuel Jiménez-Estrada, Marcos R. Escobedo-Guerra, Gabriel Arteaga-Troncoso et al.Chlamydophila abortus is one of the pathogens which induce abortion in small ruminants; this pathogen has a tropism for ruminant placenta and causes the disease commonly referred to as Ovine Enzootic Abortion (OEA). In Europe are estimated economic losses of around 20 million pounds a year by OEA. In the American Continent the disease has been reported only in Canada, … …American Journal of Animal and Veterinary Sciences, Vol. 3, Issue 4 · 31 Dec 2008 · 7,887 accessesView PDFCite
Research ArticleOpen accessIn the abstractImproving a PCR-Based Method for Identification of Ralstonia solanacearum in Natural Sources of West MalaysiaReza Khakvar, Kamaruzaman Sijam, Wong Mui Yun et al.Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it’s considered as a plant pathogen, R. solanacearum behaves primarily as a saprophytic bacterium able to survive for long periods of time in various … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 9,469 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Identification of Listeria monocytogenes in Processed Meat by a Combined Cultural-molecular MethodAngela Ingianni, Marta Quartuccio, Maria Antonietta Madeddu et al.The isolation and identification of Listeria monocytogenes in processed meat samples by a combined cultural-molecular method is described. It allows the identification of Listeria strains by means of a hybridization technique with a specific DNA probe directed to the listerial internalin gene. The specificity of this method was found to be 100% and sensitivity was as low as 1 CFU/2.5 … …American Journal of Infectious Diseases, Vol. 3, Issue 3 · 30 Sep 2007 · 7,252 accessesView PDFCite
Research ArticleOpen accessIn the abstractApplications of Real-time Reverse Transcription Polymerase Chain Reaction in Clinical Virology Laboratories for the Diagnosis of Human DiseasesR. Manojkumar, V. MrudulaEarly diagnosis that prevents further expansion of the causative agent and thereby aids control and eradication of infectious agents is critical for countries trying to obtain a particular disease free status. In these diagnostics speed is paramount, including the assay’s applicability, sensitivity, specificity, cost effectiveness and patentability. Real-time Reverse Transcription PCR (rRT-PCR) has revolutionized the field of molecular biology and … …American Journal of Infectious Diseases, Vol. 2, Issue 4 · 31 Dec 2006 · 6,553 accessesView PDFCite
Research ArticleOpen accessIn the abstractSoluble Tumor Necrosis Factor Receptor Type I and Dranulysin as Immunological Markers in Congestive Heart FailureSimone de Lima e Silva Deo, Maria Do Carmo Valente De Crasto, Alda Maria Da-Cruz et al.Previous studies have shown that circulating levels of cytokines are increased in patients with congestive heart failure (CHF), resulting in myocardial depression. In this work, we have determined serum levels of TNF-α and IFN-γ by ELISA, detected sTNFR-I by dot ELISA, as well as TNFα, IL-6, IL-10 and granulysin mRNA in unstimulated peripheral blood mononuclear cells (PBMC) by RT-PCR in … …American Journal of Immunology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,773 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation, Identification and Genomic Analysis of Plesiomonas shigelloides Isolated from Diseased Percocypris pingi (Tchang, 1930)Lei Pan, Shuiyi Liu, Xuwei Cheng et al.Recently, the outbreak of a serious infectious disease of unknown etiology was noted in Percocypris pingi (Tchang, 1930) farms in Yunnan province. Due to currently limited information, we aimed to identify the pathogen isolates, determine the susceptibility of the isolates, evaluate the pathogenicity and analyze the genome of the representative strain. Ten strains of Gram-negative rods were isolated from diseased … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 22 Dec 2017 · 8,836 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrevalence of Escherichia Coli in Some Selected Foods and Children Stools with Special Reference to Molecular Characterization of Enterohemorrhagic StrainR. Farhan, S. Abdalla, H. A. Abdelrahaman et al.Escherichia coli is one of the most important etiologic agents of childhood diarrhea that represents a major public health problem in developing countries and now is being recognized as emerging entero-pathogens in the well developed countries. Among the diarrhegenic Escherichia Coli (DEC) this work was focused on the Enterohemorrhagic Escherichia Coli (EHEC) that produce Shigatoxins (Stxs). The infection is mainly … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 4 · 23 Dec 2014 · 7,873 accessesView PDFCite
Research ArticleOpen accessIn the abstractESCHERICHIA COLI PATHOTYPES ASSOCIATED WITH DIARRHEA IN HUMAN AND DOMESTIC ANIMALSI.I. ShabanaRuminants are important reservoirs for zoonotic pathogenic E. coli. The objective of this study was to characterize pathogenic E. coli isolates from cattle and sheep linked to human illness with respect to their path types, serotypes and genotypes. E. coli O157:H7 isolated from cattle, sheep and human patients were compared for their genomic similarity by Pulsed-Field Gel-Electrophoresis (PFGE). PCR detection … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 3 · 2 Sep 2014 · 7,362 accessesView PDFCite