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Research ArticleOpen accessIn the abstractELISA-Based Development of Functional Antibodies for the Efficient Detection of Mycotoxigenic Fusarium (F. oxysporum and F. circinatum) and Diplodia (D. pinea) SpeciesPhumzile Mkhize, Phetole MangenaFusarium contains the most toxigenic and important fungal pathogens, producing a wide range of mycotoxins that include the more toxic secondary metabolites called fumonisins. This study analyzed the presence of common exo- and mycelium-soluble antigens, purified proteins using reducing SDS-PAGE and the expression of antibodies against different fungal pathogens. The results showed the presence of common antigens between pathogens and … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 18 Nov 2020 · 6,602 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro Antagonistic Antifungal-Activity of Streptomyces Isolate 339 Against Magnaporthe oryzaeM. Ebrahimi Zarandi, G. H. Shahidi Bonjar, F. Padasht DehkaeiBiological control offers an environmentally friendly alternative to the use of antimicrobials for controlling plant diseases. A collection of about 100 Actinomycete strains was screened for the ability to produce metabolites that inhibit Magnaporthe oryzae growth in vitro. The Streptomyces isolate 339 showed strong in vitro antagonistic activity against M. oryzae in Agar disc and Well-diffusion methods by producing extracellular … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 3 · 4 Sep 2013 · 8,143 accessesView PDFCite
Research ArticleOpen accessIn the abstractBIOCHEMICAL, NUTRIENT AND INHIBITORY CHARACTERISTICS OF STREPTOMYCES CULTURED FROM A HYPERSALINE ESTUARY, THE LAGUNA MADRE (TEXAS)Luis E. Espinoza, Anita L. Davelos Baines, Kristine L. LoweStreptomyces are common soil bacteria that produce secondary metabolites, including several antibiotics; however, the characteristics of marine Streptomyces are largely unknown. Sediment samples were taken from 3 sites in the Laguna Madre to isolate marine Streptomyces. Sediment was diluted, spread onto synthetic seawater media to estimate the total bacterial density of the samples and spread onto starch casein agar to … …OnLine Journal of Biological Sciences, Vol. 13, Issue 1 · 7 Feb 2013 · 7,222 accessesView PDFCite
Research ArticleOpen accessIn the abstractGC-MS Analysis and PLS-DA Validation of the Trimethyl Silyl-Derivatization TechniquesKamalrul Azlan Azizan, Syarul Nataqain Baharum, Habtom W. Ressom, Normah Mohd. NoorProblem statement: Comparison and validation analysis of the conventional and power-adjusted Microwave-Assisted (MA) techniques of TMS derivatization using Gas Chromatography-Mass Spectrometry (GC-MS) and Multivariate Analysis (MVA) of Partial Least Squares Analysis (PLS-DA). Further improvement of the conventional technique using vigorous shaking was tested and analyzed. Approach: Cross-validation and response permutation test of PLS-DA and S-plot of Orthogonal Partial Least Squares … …American Journal of Applied Sciences, Vol. 9, Issue 7 · 4 Jun 2012 · 10,325 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro Antagonist Action of Trichoderma Strains Against Sclerotinia sclerotiorum and Sclerotium cepivorumFrancisco Daniel Hernandez Castillo, Angelica Maria Berlanga Padilla, Gabriel Gallegos Morales et al.Problem statement: Sclerotinia sclerotiorum and Sclerotium cepivorum are soil pathogens which have generated resistance to synthetic fungicides. One biological alternative to this problem is to select novel strains of Trichoderma with different mechanisms of action against these plant pathogens to ensure efficient control. Approach: In the present research was determined the antagonism effect of Mexicans Trichoderma strains on S. sclerotiorum … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 3 · 16 Aug 2011 · 9,830 accessesView PDFCite
Research ArticleOpen accessIn the abstractMetabolic Fingerprinting of Three Malaysian Ginger (Zingiber officinale Roscoe) Using Gas Chromatography-Mass SpectromertryH.J. Mahdi, R. Andayani, IshakProblem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a genetic factor rather than environmental or intrinsic factors. Approach: A comprehensive metabolic fingerprinting from the leaves of three micro-propagated ginger cultivars Bukit Tinggi, Tanjung Sepat … …American Journal of Applied Sciences, Vol. 7, Issue 1 · 31 Jan 2010 · 7,390 accessesView PDFCite
Research ArticleOpen accessIn the abstractDrug Interaction between Salinomycin and Exogenous Adrenaline in DogsHamid Rajaian, Javad Sajedianfard, Saeed Nazifi, Ali Moosavi KianProblem statement: Salinomycin is a member of ionophore compounds that may be used in ruminants and pigs as growth promoter or to prevent coccidiosis. There are some reports with respect to the in vitro and in vivo sympathomimetic cardiovascular effects of salinomycin. Available reports indicate that adrenergic impact of the ionophore is brought about by an increase in the secretion … …American Journal of Pharmacology and Toxicology, Vol. 4, Issue 2 · 30 Jun 2009 · 7,544 accessesView PDFCite
Research ArticleOpen accessIn the abstractPlasmid-mediated Detoxification of Mycotoxin Zearalenone in Pseudomonas Sp. ZEA-1Abdullah D. AltalhiThe Pseudomonas sp. Strain ZEA-1 was isolated from rhizosphere of corn plant by an enrichment technique showed capability of utilizing zearalenone as the sole source of carbon. The bacterium rapidly utilized zearalenone beyond 200 µg/ml and showed prolific growth in a minimal medium containing 100 µg/ml zearalenone source. The course of ZEA degradation as well as the formation of its … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 7,862 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Postgenomic Era: Transcriptomic as the New Paradigm of LifeBruno Giardina, Marcella Renis, Vito Latora, Salvatore MusumeciNow that complete genome sequences are available for a variety of organism, the elucidation of single gene function involved in the metabolism, includes necessarily a better understanding of the cellular responses on all levels of gene products, mRNA, proteins and metabolites. Such knowledge’s growth is essential since the observable properties of living organisms, so called phenotypes, are the results of … …OnLine Journal of Biological Sciences, Vol. 6, Issue 1 · 30 Jun 2006 · 6,826 accessesView PDFCite
Research ArticleOpen accessIn the abstractDiosgenin Biosynthesis in the Sprouts of Fenugreek as Influenced by ChitosanDo Yeon Kwon, Jae Kwang Kim, Sang Un ParkTrigonella foenum-graecum L. (Fabaceae), known as Fenugreekis an annual herb, contains various secondary metabolites including steroidal saponins, flavonoids, phenolic compounds and alkaloids. This study was planned to investigate the effect of concentrations of chitosan on growth, expression of diosgenin biosynthetic genes and diosgenin accumulation in sprouts of T. foenum-graecum. Results revealed that there is no positive response of chitosan in … …OnLine Journal of Biological Sciences, Vol. 19, Issue 2 · 2 May 2019 · 7,520 accessesView PDFCite