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53 results for “Enzymatic Activity”
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Research ArticleOpen accessIn the abstractConcentration and Physicochemical Properties of Chitosan Derivatives Determine the Induction of Defense Responses in Roots and Leaves of Tobacco (Nicotiana tabacum) PlantsAlejandro B. Falcón-Rodríguez, Juan C. Cabrera, Eduardo Ortega, Miguel Ángel Martínez-TéllezProblem statement: The chitosan derivatives promote diverse defensive responses in plants, which are affected by chitosan chemical features and concentration. Glucanase (EC 3.2.1.6), Phenylalanine Ammonia-Lyase (PAL, EC 4.3.1.5) and peroxidase (POD, EC 1.11.1.6) are key enzymes in tobacco defense responses. Thus, the aim of this study was to know the behavior of their enzymatic activity in leaves and roots of … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 3 · 30 Sep 2009 · 9,263 accessesView PDFCite
Research ArticleOpen accessIn the abstractProtective Effects of Covalent Cross-Linking on Proteolysis of Human Coproporphyrinogen Oxidase and Implications for PorphyriaAhmed W. Jafri, Jason R. Stephenson, Justin B. Morgenthaler et al.The effects of covalent cross-linkers on the enzyme, coproporphyrinogen oxidase, had been previously studied but their role in protecting the enzyme from protease cleavage has not been evaluated. Therefore, we examined how the cross-linker bis (sulfosuccinimidyl) suberate (BS3) affects the ability of trypsin to digest purified, wild type recombinant human coproporphyrinogen oxidase and selected mutants. Following incubation, the apparent molecular … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,687 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Novel Rodent Model of Autism: Intraventricular Infusions of Propionic Acid Increase Locomotor Activity and Induce Neuroinflammation and Oxidative Stress in Discrete Regions of Adult Rat BrainDerrick F. MacFabe, Karina Rodríguez-Capote, Jennifer E. Hoffman et al.Innate neuroinflammatory changes, increased oxidative stress and disorders of glutathione metabolism may be involved in the pathophysiology of autism spectrum disorders (ASD). Propionic acid (PPA) is a dietary and gut bacterial short chain fatty acid which can produce brain and behavioral changes reminiscent of ASD following intraventricular infusion in rats. Adult Long-Evans rats were given intraventricular infusions of either PPA … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 2 · 30 Jun 2008 · 12,613 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Computer Use on Human Salivary Oxidant/Antioxidant Statusİmge B. Ergüder, İlker DurakComputers are widely used in recent years but their effects on human health completely are known. The aim of this study was to investigate possible effects of computer use on human salivary oxidant / antioxidant system. Ten subjects were included in the study. Three saliva samples were taken from the subjects. First was obtained before computer use, second and third … …OnLine Journal of Biological Sciences, Vol. 6, Issue 1 · 30 Jun 2006 · 6,386 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Feed Additives on the Development of Proteolytic Enzymes of the Tropical Sport Fish Malaysian Mahseer (Tor tambroides-Bleeker) FryK.C.A. Jalal, M. A. Ambak, M.A.B. Abol et al.Tor tambroides fry with a mean of 8.0±0.72 mm standard length (SL) and weighing (W) 0.06±0.01g were stocked at the rate of fifty (50) individuals in each of the fifteen 150l rectangular fibre glass tanks for a period of 5 weeks. The development of proteolytic enzymes (Trypsin and Chymotrypsin) was studied during these feeding treatments. 45% protein diet without additives … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,999 accessesView PDFCite
Research ArticleOpen accessIn the abstractUse of Cross-linking to Assess Subunit Interaction of Recombinant Human Coproporphyrinogen OxidaseJason R. Stephenson, Nancy E. Thomas, Jon A. Friesen, Marjorie A. JonesTo provide further evidence for a dimeric form of coproporphyrinogen oxidase reported using the conventional hydrodynamic methods, bifunctional cross-linkers were incubated with purified, recombinant human coproporphyrinogen oxidase to determine subunit interaction in solution. The use of cross-linkers provides an effective way to demonstrate subunit association and allows for assessment of activity upon covalent cross-linking. Following incubation with selected cross-linkers, enzyme … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,594 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled AdsorbentsQing Yang, Xuyu Lei, Jianqiang Xu, Lijia AnThe gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point of view of high-level and soluble expression, we herein constructed a recombinant gloshedobin without His-tag and developed a novel egg yolk antibody (IgY)-immunoaffinity chromatography for its purification in a … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,690 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordEffects of Soil Storage at Freezing Temperatures on Soil Enzymatic ActivitiesAinsley Lougheed, Kabwe NkongoloSoil enzyme activities are good indicators of soil health. It has been hypothesized that soil storage before analysis might affect microbial function. The objective of the present study was to determine if soil storage at -20 and -80°C affects soil enzymatic activities. Soil samples were collected and stored for four weeks at -20 and -80°C. Activities of nine enzymes were … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 12 Aug 2024 · 4,984 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordThe Enzymatic Role of Lipid Metabolism in Yarrowia lipolytica Grown on GlycerolRuixue Wang, Feifei Xin, Yufei Chang et al.More attention has been paid to the production of commercial fatty acids by microbial fermentation. The basic biochemistry of lipid accumulation in oleaginous microorganisms under nitrogen limitation has been extensively studied. As an oleaginous microorganism, Yarrowia lipolytica can produce fatty acid-derived biofuels and biochemicals. However, the response of the enzymes in Y. lipolytica to glycerol remains unclear. Therefore, we aimed … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 2 · 17 Apr 2023 · 6,309 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordProposed Insights in the Pharmaco-Dynamic Theory of Ascorbate Pro-Oxidant ActivityKheir Eddine Kerboua, Fatma Haiba, Fatima Bendjemia et al.Ascorbate pro-drug theory promising possible effective and nontoxic treatment is intensively examined and centered on the importance of both extracellular and intra-cellular effects of ascorbate. In this study, we sought to determine the in vitro cell responsiveness in a plasma free milieu with or without an extracellular catalyst and/or hydrogen peroxide. This project brought to light how ascorbic acid would … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 24 Nov 2015 · 6,966 accessesView PDFCite