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53 results for “Affinity”
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Research ArticleOpen accessIn the abstractBiotransformation Using Recombinant Cmp Sialic Acid Synthetase and α-2, 6-Sialyltran Sferase: Enzymatic Synthesis of SialosidesUlrike Hubl, Cynthia Sun, Shuguang Zhang et al.In this research, we successfully expressed recombinant CMP-sialic Acid Synthetase (CSS) from Neisseria meningitides and 2,6-Sialyltransferase (SAT) from Photobacterium damsela in E. coli BL21(DE3) fermented at a scale of up to 8 litres using individual plasmids pIRL-1 and pIRL-4b, respectively. After cell lysis with BugBuster, enzyme levels of 2U and 22U per litre were produced for CSS and SAT, respectively. … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 8,854 accessesView PDFCite
Research ArticleOpen accessIn the abstractDihydrobiopterin (BH2): Key Determinant in Influencing Arginine Mediated Endothelial Tolerance and DysfunctionSrinidi Mohan, Harsh Patel, Jorge Bolinaga et al.The redox-sensitive tetrahydrobiopterin (BH4) is an essential cofactor that is required by endothelial Nitric Oxide Synthase (eNOS) for L-arginine (ARG) mediated Nitric Oxide (NO) generation. Oxidation of BH4 causes cofactor insufficiency and uncoupling of eNOS, resulting in product switching from NO to O2⋅– production. Here we tested the hypothesis that eNOS uncoupling is not simply a consequence of BH4 insufficiency, … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 8,348 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Correlations Between Mercury Speciation and Dissolved Organic Matter in the Sediment of the Red SeaM. A. Wedyan, F. A. Ababneh, S. Al-RousanThis study addresses the correlations between Hg and organic matter in recent sediment; samples were collected from the Gulf of Aqaba, Red Sea coasts (oligotrophic regions) during 2010. In the present study TOC analyzer was used to determine Total Organic Carbon (TOC) and Total Nitrogen (TN) concentrations and the total amount of mercury (HgT) in sediment samples were analyzed by … …American Journal of Environmental Sciences, Vol. 8, Issue 4 · 21 Jun 2012 · 6,795 accessesView PDFCite
Research ArticleOpen accessIn the abstractLow Pass Filter Model for Chemical Sensors in Response to Gases and OdorsMahmoud Z. IskandaraniDesign and Modeling multi-gap sensing odor system for the objectives of odor recognition, classification and correlation are carried out. The model illustrates the low pass functionality of the multi-gap sensor acting as a filter for odors. Problem statement: Odor filtering is an important issue in today's world. In addition knowing the original material that an odor belongs to even after … …American Journal of Applied Sciences, Vol. 9, Issue 4 · 17 Feb 2012 · 6,809 accessesView PDFCite
Research ArticleOpen accessIn the abstractChildhood Cancer-a Hospital based study using Decision Tree TechniquesK. Kalaivani, R. ShanmugalakshmiProblem statement: Cancer is generally regarded as a disease of adults. But there being a higher proportion of childhood cancer (ALL-Acute Lymphoblastic Leukemia) in India. The incidence of childhood cancer has increased over the last 25 years, but the increase is much larger in females. The aim was to increase our understanding of the determinants of south Indian parental reactions … …Journal of Computer Science, Vol. 7, Issue 12 · 17 Oct 2011 · 7,057 accessesView PDFCite
Research ArticleOpen accessIn the abstractInheritance based Intelligent Technique Employing Nested-XOR with Recursion for Recognition and Classification of Odors using Multi-Sensor Nose SystemMahmoud Z. IskandaraniProblem statement: Odor originality and fraud is an important issue in today’s world. In addition knowing the original material that an odor belongs to even after being mixed with others is also of vital importance. In addition measuring quality of mixed odors in terms of their affinity and belonging to a specific category or is critical. Approach: Design, testing and … …American Journal of Applied Sciences, Vol. 8, Issue 9 · 2 Aug 2011 · 6,676 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction and Purification of Collagenase Enzymes: A Critical ReviewSaid M Daboor, Suzanne M Budge, Abdel E Ghaly et al.Problem statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low concentrations. Recent efforts to identify new sources of useful enzymes have been concentrated on the marine environment because of the potential to make use of processing wastes. About 35-50% of the mass of … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 14,311 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparative Docking Analysis on Natural Compounds Versus a Synthetic Drug as a Therapeutic for Acquired Immuno Deficiency SyndromeSandhya R. Nair, R. Subhashini, B. ThiagarajanProblem statement: Acquired Immune Deficiency Syndrome (AIDS) is one of the worst pandemics affecting and spreading quickly all over the world with a high mortality rate. AIDS has been able to kill so many people due to the unique property of Human Immunodeficiency Virus (HIV) virus. It attacks the immune system of the victim there by not allowing the immune … …Current Research in Medicine, Vol. 1, Issue 2 · 31 Oct 2010 · 7,323 accessesView PDFCite
Research ArticleOpen accessIn the abstractUterus-Relaxing Study of a Sudanese Herb (El-Hazha)Aimun Abdelgaffar Elhassan Ahmed, Robert Gaspar, Arpad Marki et al.Problem statement: The aim of this study is to investigate the pharmacological effects of the Methanolic-extract (AH2) of El-Hazha and its sub-fractions. Approach: These investigations were carried out on in vitro isolated uterus preparations from Non-Pregnant (NP) and Late-Pregnant rats (LP). In parallel displacement radio-ligand binding assay was performed for β-Adrenergic Receptors (β-ADR). Results: Showed that the herb and its … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,509 accessesView PDFCite
Research ArticleOpen accessIn the abstractLectin Histochemistry Assay in Colon Tissues for Inter-species CharacterizationFaezeh Yazdani Moghaddam, Jamshid Darvish, Nasser Mahdavi Shahri et al.Problem statement: The digestive tract of animals including rodents has taxonomic and ecologic importance due to its ability in adaptation for different environments. Whereas colon absorbs water and carbohydrates, it has a vital role ecologically and physiologically and enjoys different histological structures in different rodents with various diets. Approach: Different rodent specimens belong to the family Muridae, namely Gerbilinae subfamily … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 1 · 31 Mar 2009 · 8,685 accessesView PDFCite