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Research ArticleOpen accessIn the keywordsOccurrence of Feline Immunodeficiency Virus and Feline Leukemia Virus Infection in CatsFabiana Alves, Daniela de Souza Rajao, Helen Lima Del Puerto et al.Problem statement: The objective of the present study was to evaluate occurrence of the Feline Immunodeficiency Virus (FIV) and the Feline Leukemia Virus (FeLV) infection in asymptomatic domestic cats in the metropolitan region of Belo Horizonte, Minas Gerais, using the Polymerase Chain Reaction (PCR) for FIV detection and SNAP Combo Plus for FeLV and FIV detection. Approach: Blood samples were … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 3 · 2 Nov 2011 · 8,721 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEarly Diagnosis of Breast Cancer using Molecular, Biochemical and Pathological MarkersSalah El-Din El-Assal, Adel A. El-Tarras, Samir M. Abd-allaProblem statement: Laboratory diagnosis of breast cancer in most of the hospitals has traditionally been performed using cell culture and the direct hormone receptor assay, which are money and time consuming. Approach: This study was performed in order to direct the attention toward increasing the efficiency of early diagnosis in clinical laboratories at the western region of KSA and Egypt … …American Journal of Applied Sciences, Vol. 8, Issue 1 · 31 Jan 2011 · 8,060 accessesView PDFCite
Research ArticleOpen accessIn the keywordsExpression of Adiponectin Receptors in Human Placenta and Its Possible Implication in Gestational DiabetesNaglaa Fathy Al Husseini, Mosaad M. Odaa, M.A. Mohamed et al.Problem statement: Similar to obese patients and type 2 diabetic patients, adiponectin levels are reduced in former Gestational Diabetes Mellitus (GDM) patients and are lower in GDM women during late pregnancy compared with pregnant control subjects matched for BMI. Diabetic insult at later stages in gestation, such as may occur in gestational diabetes, will foremost lead to short-term changes in … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 8,199 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMolecular Gender Determination of Ancient Human from Malay PeninsularZ.A. Shahrul Hisham, S. Sahidan, M. A.W. Rohaya et al.Problem statement: DNA samples from fourteen modern human bloods (seven males and seven females) and two ancient skeletal samples excavated from Kalumpang Island and Dungun, Peninsular Malaysia were subjected for molecular genders determination using specific primers of human AMELX and AMELY. Approach: A standard multiple PCR mixture with forward primer and either a human X-specific reverse primer for AMELX or … …American Journal of Applied Sciences, Vol. 6, Issue 10 · 31 Oct 2009 · 7,609 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetection of Phytoplasma on Indian Fig (Opuntia ficus-indica Mill) in Mexico Central RegionRicardo Hernández-Pérez, Juan Carlos Noa-Carrazana, Ricardo Gaspar et al.Problem statement: The Indian fig (a species of prickly pear cactus), has been known as Nopal, comprises an approximate area of 100,000 ha, in plantations used for human consumption. "Pyramids" Indian fig area located in the northeastern State of Mexico has been an important Indianfig area in the country, with 15810 ha, where a phytoplasma has been consistently present in … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,733 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMolecular Characterization of a Phytoplasma Associated with Coconut Yellow Decline (CYD) in MalaysiaN. Nejat, K. Sijam, S. N.A. Abdullah et al.Problem statement: Molecular methods have been used to detect phytoplasma in Malayan Red Dwarf (MRD) ecotype of coconut for the first time in Malaysia. Detect phytoplasma from coconut showing yellowing symptoms by nested PCR and Classify phytoplasma associated with disease of coconut palms, based on analysis of 16S rRNA gene operon sequences and virtual RFLP. Approach: Twenty MRD symptomatic palms … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 7,923 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMolecular Characterization of Fusarium Oxysporum F. Sp. Cubense of BananaS. K. Leong, Z. Latiffah, S. BaharuddinProblem statement: Morphological characterization of Fusarium species which emphasize on microscopic and cultural characteristics are not sufficient to characterize Fusarium Oxysporum F. sp. Cubense (FOC) from banana as these characteristics could easily influence by environmental factors. As an alternative molecular methods were used to characterize and to assess genetic variation of FOC from different banana cultivars. Knowledge on the genetic … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 9,272 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMolecular Analysis of Coagulase Gene Polymorphism in Clinical Isolates of Methicilin Resistant Staphylococcus aureus by Restriction Fragment Length Polymorphism Based GenotypingM. Himabindu, D. Sugapriya Muthamilselvan, Dillip Kumar Bishi, Rama S. VermaProblem statement: To investigate coagulase gene variants through Restriction Fragment Length Polymorphism (RFLP) amongst clinical isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA) collected from Chennai based hospitals. Approach: A total of 85 MRSA clinical isolates collected from various clinical sources were studied for the molecular typing purpose. To perform coagulase gene typing, the repeated units encoding hypervariable regions of the Staphylococcus … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,767 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPrevalence of Human Papillomavirus Types Among Mexican Women with Intraepithelial Lesions and Cervical Cancer: Detection with MY09/MY011 and GP5+/GP6+ Primer SystemsGloria Fernández-Tilapa, Berenice Illades-Aguiar, Dinorah Nashely Martínez-Carrillo et al.Squamous cervical carcinoma (SCC) is the most common cancer found in Mexican women. Human papillomavirus (HPV) infection is a prerequisite for this disease. In Mexico little is known about the prevalence of HPV-types and knowledge of the circulating genotypes by region is limited. The aim of this study was to determine the prevalence and genotypes of HPV in biopsies from … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 7,309 accessesView PDFCite
InvestigationOpen accessIn the keywordsMeaning of the Oral Hygiene in Children, Due to Atherosclerosis GenerationAnna Wójtowicz-Bujak, Andrzej WolskiResearch focused on association between periodontal diseases and atherosclerosis has been substantial in recent years. It is now well known that chronic inflammation plays an important role in initiation and evolution of atherosclerosis. The bacterial plaque is a key pathology leading to periodontal inflammation. As reported by other authors, bacteria causing periodontal disease were found within the carotid arteries in … …American Journal of Infectious Diseases, Vol. 11, Issue 4 · 7 Nov 2015 · 7,205 accessesView PDFCite