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59 results for “molecular identification”
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EditorialOpen accessIn the abstractRe-Paving the Road Built by Chemistry: A Challenge to Biochemistry and BiotechnologyAlberto A. IglesiasBiochemistry’s birth can be placed at the end of the nineteenth century, when Eduard Buchner made a clever interpretation of experimental observations on the generation of gas bubbles after addition (the original purpose was preservation) of sucrose to a yeast crude extract (Lagerkvist, 2005). Intelligently, Buchner concluded that the disaccharide had been fermented by broken cells’ suspension to produce CO2, … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 1 · 26 Jan 2015 · 7,468 accessesView PDFCite
Research ArticleOpen accessIn the abstractNovel Biomarkers in Determining Prostate Cancer Diagnosis and PrognosisZuzana Varchulova Novakova, Katarina Bevizova, Iveta GasparovaThe clinical behavior and molecular pathology of prostate cancer is highly variable. Current "traditional" prognostic markers cannot reliably distinguish the potentially life-threatening cancer from indolent cancer. Identification of additional new predictors of cancer aggressiveness is therefore urgently required. This communication is aimed at a brief review of new biomarkers in prostate cancer diagnostics and prognostics. Pubmed systematic search was performed … …OnLine Journal of Biological Sciences, Vol. 14, Issue 4 · 14 Jan 2015 · 6,905 accessesView PDFCite
Research ArticleOpen accessIn the abstractMOLECULAR CHARACTERIZATION OF SARCOCYTIS FUSIFORMIS AND SARCOCYSTIS BUFFALONIS INFECTING WATER BUFFALOES (BUBALUS BUBALIS) FROM EGYPTMahmoud El-Seify, Ahmed El-Morsey, Mosaad Hilali et al.Parasites of the genus Sarcocystis have the ability to infect wide range of animals, birds and reptiles. Water buffaloes are intermediate hosts for four Sarcocystis species; S. fusiformis, S. buffalonis, S. levinei and S. dubeyi. There was no previous molecular identification of Sarcocystis spp. parasitizing water buffaloes from Egypt. The current study was established to screen infection rates and apply … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 2 · 16 Apr 2014 · 7,943 accessesView PDFCite
Research ArticleOpen accessIn the abstractMOLECULAR CHARACTERIZATION AND IMMUNOPROTECTIVE ACTIVITY OF CAPSULAR POLYSACCHARIDE OF KLEBSIELLA PNEUMONIAE ISOLATED FROM FARM ANIMALS AT TAIF GOVERNORATEAhmed M.A. Mansour, Hoda M. Zaki, Nibal A. Hassan, Abdulrahman A. Al-HumianyKlebsiella pneumoniae is a Gram-negative enterobacterium that has historically been and currently remains, a significant cause of human disease and several kinds of infections in animals. In the present work, trials for the isolation of Klebsiella pneumoniae from diseased and apparently healthy farm animals (cows, sheep, goats and camels) were done for recognition of Klebsiella pneumoniae subspecies. It was noticed … …American Journal of Infectious Diseases, Vol. 10, Issue 1 · 6 Jan 2014 · 7,942 accessesView PDFCite
Research ArticleOpen accessIn the abstractHost Pathogen Interaction Study in Malformed Affected Tissues of Mangifera indica L.Pradeep Kumar, Madhu Kamle, Vijai Kumar Gupta et al.The malformation disease of mango by Fusarium moniliforme var. subglutinan is one of the major causes of huge economical loss every year. Early diagnosis of the pathogens can help cultivators to protect the mango orchid and fruits from successive and secondary infection. The molecular detection is one of the better choices among available techniques for diagnosis. But the farmers or … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 3 · 4 Sep 2013 · 7,922 accessesView PDFCite
Research ArticleOpen accessIn the abstractRapid Real Time PCR Based Detection of Cell Count in Case of Urinary Tract InfectionPoulomi Nandy, Ashoke Ranjan Thakur, Shaon Ray ChaudhuriMicrobial identification and antimicrobial susceptibility testing methods currently used in clinical microbiology laboratories require at least two to three days because they rely on the growth and isolation of micro-organisms. This long, but necessary, delay has enormous consequences on prophylactic usage of antimicrobial drugs. This study was an attempt to reduce this detection time span. Taq Man Real Time PCR … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 2 · 22 Jun 2013 · 8,217 accessesView PDFCite
Research ArticleOpen accessIn the abstractPreliminary Study of Phylogenetic Relationship of Rice Field Chironomidae (Diptera) Inferred From DNA Sequences of Mitochondrial Cytochrome Oxidase Subunit ISalman A. Al-Shami, M.N. Siti Azizah, Che Salmah Md Rawi, Abu Hassan AhmadProblem statement: Chironomidae have been recorded in rice fields throughout the world including in many countries such as India, Australia and the USA. Although some studies provide the key to genera level and note the difficulty of identifying the larvae to species level. Chironomid researches have been hindered because of difficulties in specimen preparation, identification, morphology and literature. Systematics, phylogenetics … …American Journal of Applied Sciences, Vol. 6, Issue 5 · 31 May 2009 · 7,524 accessesView PDFCite
Research ArticleOpen accessIn the abstractAn Inducible System for the Identification of Target Genes for a Regulator in MycobacteriaShruti Jain, Garima Khare, Pushplata Tripathi, Anil K. TyagiWe described principle and application of an inducible system for identification of target genes of a given mycobacterial protein with a regulatory function. This vector system, a promoterprobe vector, carries (i) promoterless lacZ as the reporter gene and (ii) the mycobacterial gene encoding the regulatory protein under the transcriptional control of the promoter of highly inducible mycobacterial gene encoding acetamidase. … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 7,255 accessesView PDFCite
Research ArticleOpen accessIn the abstractMicrobial Biodiversity Screening for Metal Accumulators from Mineral Ore Rich Site in Andhra Pradesh, IndiaAditya Kumar Sarkar, Susmita Roy, Ananya Pal et al.Problem statement: Since the site selected for screening was a source of magnetite, the objective of this study was to isolate some groups of magnetotactic bacteria and microbes with potential of metal accumulation. Approach: The culture based approach was used for the study. Pure isolates obtained were characterized on the basis of morphology, biochemical tests, physiology as well as at … …OnLine Journal of Biological Sciences, Vol. 8, Issue 2 · 30 Jun 2008 · 7,553 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification of Methicillin Resistant Staphylococcus aureus (MRSA) and Methicillin Resistant Coagulase-Negative Staphylococcus (CoNS) in Clinical SettingsM.A. Shorman, A.M. Atoom, N.M. Abuharfeil, A.M. Al-MajaliTo evaluate different methods for the identification of methicillin-resistant Staphylococci and their reliability, 112 Staphylococcal isolates (32 Staphylococcus aureus isolates and 80 coagulase negative Staphylococci isolates "CoNS") were collected from 118 nasal swab cultures and were subjected to three methods to detect oxacillin susceptibility of the isolates. The three methods were oxacillin disk diffusion method; the Epsilometer- test (E-test) and … …American Journal of Infectious Diseases, Vol. 4, Issue 2 · 30 Jun 2008 · 7,978 accessesView PDFCite