Sign inSubmit
Tips: “quotes” for a phrase · author:Lee · a DOI goes straight to its article

40 articles for “Western blot”
Set a search alert
Research ArticleOpen accessIn the abstractHistone Tail Modifications of H3 and H4 during the Physarum polycephalum Cell CycleMarie-Claire Arnaud, Christophe ThirietProblem statement: Histone modifications are required for chromatin activities and are believed to form an epigenetic code. However, the inheritance of the histone post-translational modifications remains unclear. Approach and Results: The aim of this study was to evaluate the histone modifications throughout the cell cycle and to determine whether the histone marks are transferred to daughter cells. Using the naturally … …OnLine Journal of Biological Sciences, Vol. 12, Issue 2 · 29 May 2012 · 7,580 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnti-Metalloproteinase-9 Activities of Selected Indonesian Zingiberaceae Rhizome Extracts in Lipopolysaccharide-Induced Human Vascular Endothelial Cells In VitroYantiProblem statement: Atherosclerosis is associated with chronic inflammation triggered by bacterial infection that activates the breakdown of extracellular matrix protein by matrix Metalloproteinases (MMPs). Zingiberaceae, a group of tropical food crops grown in Indonesia and other Southeast Asia regions, has been traditionally used for food coloring, seasoning, culinary and medicinal purposes. However, its efficacy as natural vascular protection has not … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 1 · 27 May 2011 · 7,869 accessesView PDFCite
Research ArticleOpen accessIn the abstractInhibition of U6 snRNA Transcription by PTENStephanie Cabarcas, Kounosuke Watabe, Laura SchrammProblem statement: RNA polymerase III (RNA pol III) is responsible for transcribing many of the small structural RNA molecules involved in RNA processing and protein translation, thereby regulating the growth rate of a cell. RNA pol III transcribes both gene internal (tRNA) and gene external (U6 snRNA) promoters and proper initiation by RNA polymerase III requires the transcription initiation factor … …OnLine Journal of Biological Sciences, Vol. 10, Issue 3 · 30 Sep 2010 · 8,551 accessesView PDFCite
Research ArticleOpen accessIn the abstractLipoprotein (a) and Apolipoprotein (a) Isoforms in Patients with Acute Myocardial InfarctionAkram Saleh, Izzat AL-Awwaa, Sally Awwadeh, Nabil BashirProblem statement: The objective of this study is to determine the relationship between plasma lipoprotein (a) levels and apolipoprotein (a) isoforms in a group of Jordanian patients with Acute Myocardial Infarction (AMI) Approach: A total of 90 patients with acute myocardial infarction were compared with 90 age-and sex-matched controls. Lipoprotein (a) levels were measured by ELIZA method and isoforms were … …Current Research in Medicine, Vol. 1, Issue 1 · 30 Jun 2010 · 7,535 accessesView PDFCite
Research ArticleOpen accessIn the abstractCocaine Exposure Results in Formation of Dendritic Varicosity in Rat Primary Hippocampal NeuronsHonghong Yao, Crystal Bethel-Brown, Anil Kumar, Shilpa BuchProblem statement: It has been well documented that drugs of abuse such as cocaine can cause enhanced progression of HIV-Associated Neuropathological Disorders (HAND), the underlying mechanisms mediating these effects remain poorly understood. Approach: In present study, we explored the impact of cocaine exposure (I and 10 μM) on the dendritic beading in rat primary hippocampal neurons. Using the approach of … …American Journal of Infectious Diseases, Vol. 5, Issue 1 · 31 Mar 2009 · 7,889 accessesView PDFCite
Research ArticleOpen accessIn the abstractEpitope Mapping of Dengue-Virus-Enhancing Monoclonal-Antibody Using Phage Display Peptide LibraryChung-I Rai, Huan-Yao Lei, Yee-Shin Lin et al.The Antibody-Dependent Enhancement (ADE) hypothesis has been proposed to explain why more severe manifestations of Dengue Hemorrhagic Fever and Dengue Shock Syndrome (DHF/DSS) occur predominantly during secondary infections of Dengue Virus (DV) with different serotypes. However, the epitopes recognized by these enhancing antibodies are unclear. Recently, antipre-M monoclonal antibody (mAb 70-21), which recognized all DV serotypes without neutralizing activity, were … …American Journal of Infectious Diseases, Vol. 4, Issue 1 · 31 Mar 2008 · 8,453 accessesView PDFCite
Research ArticleOpen accessIn the abstractSignaling Pathways Involved In Dengue-2 Virus Infection Induced RANTES OverexpressionYing-Ray Lee, Huan-Yao Lei, Shun-Hua Chen et al.Dengue viruses participate in liver inflammation by inducting the expression of various chemokines including Regulated on Activation Normal T-cell Expressed and Secreted (RANTES). However, the underlying signaling remains unknown. Here, we reveal that Ras, Raf-1 and three mitogen-activated protein kinases (MAPKs) p38, extracellular signal-regulated kinase (Erk), and c-jun-NH2-terminal kinase (JNK) can be activated or phosphorylated in dengue-2 virus infected hepatocyte … …American Journal of Infectious Diseases, Vol. 4, Issue 1 · 31 Mar 2008 · 7,173 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression of Toll-like Receptors (TLRs) and Nucleotide-binding Oligomerization Domain (NODs) in murine peritoneal macrophages on in vitro treatment with Thymosin α 1Shikha Tarang, Ajit SodhiThymosin α 1 (Tα1) treatment of murine peritoneal macrophages in vitro induces the expression of TLRs and Nod2 proteins. Enhanced expressions of TLR2, -4, -5, -6, -7, -8, -9 and Nod2 were observed by RT-PCR and western blotting. Expression of downstream signaling molecules - MyD88, IRAK1, TRAF6, IRF3 were also up regulated on treatment with Tα1. It was also observed … …American Journal of Immunology, Vol. 3, Issue 1 · 30 Jun 2007 · 7,150 accessesView PDFCite
Research ArticleOpen accessIn the abstractImatinib Mesylate Inhibits Glucose Uptake in Gastrointestinal Stromal Tumor Cells by Downregulation of the Glucose Transporters Recruitment to the Plasma MembraneHans Prenen, Cristiana Stefan, Bart Landuyt et al.Imatinib mesylate, the inhibitor of the KIT protein tyrosine kinase that is constitutively activated in Gastrointestinal Stromal Tumors (GISTs), has been established as the first highly effective drug in the treatment of patients with advanced GISTs. Recent studies suggest that changes in the glucose metabolism could be an additional mechanism of the anti-proliferative action of imatinib. The aim of this … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 7,245 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled AdsorbentsQing Yang, Xuyu Lei, Jianqiang Xu, Lijia AnThe gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point of view of high-level and soluble expression, we herein constructed a recombinant gloshedobin without His-tag and developed a novel egg yolk antibody (IgY)-immunoaffinity chromatography for its purification in a … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,690 accessesView PDFCite