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67 articles for “In vitro Culture”

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Research ArticleOpen accessIn the abstractCallus Induction and Cellular Suspensions from Murtilla (Ugni molinae Turcz.) for trans-resveratrol ProductionSalazar Carolina, Bustos Evelyn, Perez Claudia et al.The present study reports for the first time the quantification of resveratrol and the use of biotechnological techniques applied to stilbene productions in species from genus Ugni and calli production from adult explants (fruits). Resveratrol is synthesized by a series of families of higher plants, which has generated much interest in recent years for its antioxidant, anticancer and antitumor properties, … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 1 · 24 Jan 2017 · 7,942 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Serial Passaging on the Morphology and Biological Characteristics of Human Adipose Tissue-derived Stem CellsLenka Oravcova, Martin Bohac, Lubica Krajciova et al.Somatic stem cells posses an exclusive property of self regeneration and plasticity which makes them promising candidates for use in tissue engineering and regenerative medicine. In the case of stem cell therapy it is crucial to obtain them in sufficient amount. That's why they have to be cultured under in vitro conditions. Long term culture provides adequate number of cells, … …OnLine Journal of Biological Sciences, Vol. 16, Issue 4 · 17 Dec 2016 · 7,242 accessesView PDFCite
Research ArticleOpen accessIn the abstractHybrid Ceramo-Polymeric Nanocomposite for Biomimetic Scaffolds Design and PreparationRaffaella Aversa, Relly Victoria V. Petrescu, Roberto Sorrentino et al.Biomimetics, biomechanics and tissue engineering are three multidisciplinary fields that have been contemplated in this research to attain the objective of improving prosthetic implants reliability. Since testing and mathematical methods are closely interlaced, a promising approach seemed to be the combination of in vitro and in vivo experiments with computer simulations (in silico). An innovative biomimetics and biomechanics approach and … …American Journal of Engineering and Applied Sciences, Vol. 9, Issue 4 · 30 Nov 2016 · 8,269 accessesView PDFCite
Research ArticleOpen accessIn the abstractStage Specificity of Eurycomanone Isolated from Eurycoma longifolia on Plasmodium falciparum CyclesEti Nurwening Sholikhah, Mahardika Agus Wijayanti, Ratna Asmah Susidarti et al.Eurycomanone is the most active compounds in the roots of Eurycoma longifolia and shown to have in vitro antiplasmodial activity. However, the stage of Plasmodium falciparum cycles which are sensitive to eurycomanone have not been investigated. This study was conducted to investigate stage specificity of eurycomanone at various stages of P. falciparum life cycles. Stage specificity of eurycomanone at various … …American Journal of Pharmacology and Toxicology, Vol. 11, Issue 1 · 16 Jan 2016 · 7,739 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Short Term in vitro Stress Hormone Exposure on Regulatory T Cell Number and Function in AsthmaKristina E. Rehm, Gailen D. Marshall, Jr.Asthma is characterized by alterations in the immune system, including regulatory T cells (Treg). Further alterations in Treg numbers and/or function caused by stress hormones may be a contributing factor in asthma pathogenesis. We compared Treg populations and the effects of Dexamethasone (DEX, a laboratory analog of cortisol) on Treg cell number and function in patients with asthma and a … …American Journal of Immunology, Vol. 11, Issue 1 · 24 Apr 2015 · 6,608 accessesView PDFCite
Research ArticleOpen accessIn the abstractOPTIMUM MICRONUTRIENT LEVEL FOR PHALAENOPSIS DELICIOSA ORCHID SEEDLING IN VITRO GROWTHZherui Xiong, Chieh Wean ChoongThere were many researches about the influence of micronutrient concentration toward growth of plants. However, there was no clear statement about the micronutrient level for growth of Phalaenopsis seedlings. Hence it was worth to investigate the optimum level of micronutrient for Phalaenopsis, an endangered orchid species. Using Phalaenopsis deliciosa as the subject, germinated seedlings were grown on defined culture media … …OnLine Journal of Biological Sciences, Vol. 14, Issue 3 · 12 Nov 2014 · 6,629 accessesView PDFCite
Research ArticleOpen accessIn the abstractBRASSINOSTEROIDS IN BREEDING TECHNOLOGIES HEALTHCARE POTATONatalia Nikolaevna Koposova, Olga Veniaminovna Shtyrlina, Irina Pavlovna Uromova et al.The regulator of anti-stress action Epin-Extra (24-epibrassinolide) was tested in laboratory and field (protected ground) conditions. The aim of this research was to study the influence of growth regulators on the growth, development and productivity of improved initial promising varieties of potato: Native (cultivated varieties Udacha) and foreign (cultivated varieties Red Scarlett) selections obtained by the method of apical meristem … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 28 Aug 2014 · 7,322 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro Antagonistic Antifungal-Activity of Streptomyces Isolate 339 Against Magnaporthe oryzaeM. Ebrahimi Zarandi, G. H. Shahidi Bonjar, F. Padasht DehkaeiBiological control offers an environmentally friendly alternative to the use of antimicrobials for controlling plant diseases. A collection of about 100 Actinomycete strains was screened for the ability to produce metabolites that inhibit Magnaporthe oryzae growth in vitro. The Streptomyces isolate 339 showed strong in vitro antagonistic activity against M. oryzae in Agar disc and Well-diffusion methods by producing extracellular … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 3 · 4 Sep 2013 · 8,140 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro and In vivo Activity of Lactococci Strains against Helicobacter pyloriHassina Guetarni, Ahmed Bensoltane, Djamila Aichour et al.Problem statement: Search for lactic acid bacteria that have in vitro, a significant inhibitory effect against the strains of H. pylori and to determine the inhibitory activity in vivo. Approach: The in vitro inhibitory activity of lactic acid bacteria isolated from milk against strains of H. pylori was determined by the agar diffusion method. Two groups of mice were inoculated … …OnLine Journal of Biological Sciences, Vol. 12, Issue 2 · 15 May 2012 · 7,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractLemon Pepper Fruit Extract (Zanthoxylum acanthopodium DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages In VitroYanti, Theodorus Eko Pramudito, Nerissa Nuriasari, Katarina JulianaProblem statement: Lemon pepper fruits (Zanthoxylum acanthopodium DC.; Rutaceae) have been used as a traditional source against stomach ache by Batak people in North Sumatera province, Indonesia. However, its scientific evidence for treatment of inflammatory disorders particularly gastritis has not been reported. Approach: Here, we investigated the inhibitory effects of Lemon Pepper Fruit Extract (LPFE) against inflammatory biomarkers by conducting … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 14 Feb 2012 · 9,581 accessesView PDFCite