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67 results for “In vitro Culture”

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Research ArticleOpen accessIn the abstractStage Specificity of Eurycomanone Isolated from Eurycoma longifolia on Plasmodium falciparum CyclesEti Nurwening Sholikhah, Mahardika Agus Wijayanti, Ratna Asmah Susidarti et al.Eurycomanone is the most active compounds in the roots of Eurycoma longifolia and shown to have in vitro antiplasmodial activity. However, the stage of Plasmodium falciparum cycles which are sensitive to eurycomanone have not been investigated. This study was conducted to investigate stage specificity of eurycomanone at various stages of P. falciparum life cycles. Stage specificity of eurycomanone at various … …American Journal of Pharmacology and Toxicology, Vol. 11, Issue 1 · 16 Jan 2016 · 7,745 accessesView PDFCite
Research ArticleOpen accessIn the abstractOPTIMUM MICRONUTRIENT LEVEL FOR PHALAENOPSIS DELICIOSA ORCHID SEEDLING IN VITRO GROWTHZherui Xiong, Chieh Wean ChoongThere were many researches about the influence of micronutrient concentration toward growth of plants. However, there was no clear statement about the micronutrient level for growth of Phalaenopsis seedlings. Hence it was worth to investigate the optimum level of micronutrient for Phalaenopsis, an endangered orchid species. Using Phalaenopsis deliciosa as the subject, germinated seedlings were grown on defined culture media … …OnLine Journal of Biological Sciences, Vol. 14, Issue 3 · 12 Nov 2014 · 6,633 accessesView PDFCite
Research ArticleOpen accessIn the abstractBRASSINOSTEROIDS IN BREEDING TECHNOLOGIES HEALTHCARE POTATONatalia Nikolaevna Koposova, Olga Veniaminovna Shtyrlina, Irina Pavlovna Uromova et al.The regulator of anti-stress action Epin-Extra (24-epibrassinolide) was tested in laboratory and field (protected ground) conditions. The aim of this research was to study the influence of growth regulators on the growth, development and productivity of improved initial promising varieties of potato: Native (cultivated varieties Udacha) and foreign (cultivated varieties Red Scarlett) selections obtained by the method of apical meristem … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 28 Aug 2014 · 7,326 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro Antagonistic Antifungal-Activity of Streptomyces Isolate 339 Against Magnaporthe oryzaeM. Ebrahimi Zarandi, G. H. Shahidi Bonjar, F. Padasht DehkaeiBiological control offers an environmentally friendly alternative to the use of antimicrobials for controlling plant diseases. A collection of about 100 Actinomycete strains was screened for the ability to produce metabolites that inhibit Magnaporthe oryzae growth in vitro. The Streptomyces isolate 339 showed strong in vitro antagonistic activity against M. oryzae in Agar disc and Well-diffusion methods by producing extracellular … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 3 · 4 Sep 2013 · 8,143 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro and In vivo Activity of Lactococci Strains against Helicobacter pyloriHassina Guetarni, Ahmed Bensoltane, Djamila Aichour et al.Problem statement: Search for lactic acid bacteria that have in vitro, a significant inhibitory effect against the strains of H. pylori and to determine the inhibitory activity in vivo. Approach: The in vitro inhibitory activity of lactic acid bacteria isolated from milk against strains of H. pylori was determined by the agar diffusion method. Two groups of mice were inoculated … …OnLine Journal of Biological Sciences, Vol. 12, Issue 2 · 15 May 2012 · 7,731 accessesView PDFCite
Research ArticleOpen accessIn the abstractLemon Pepper Fruit Extract (Zanthoxylum acanthopodium DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages In VitroYanti, Theodorus Eko Pramudito, Nerissa Nuriasari, Katarina JulianaProblem statement: Lemon pepper fruits (Zanthoxylum acanthopodium DC.; Rutaceae) have been used as a traditional source against stomach ache by Batak people in North Sumatera province, Indonesia. However, its scientific evidence for treatment of inflammatory disorders particularly gastritis has not been reported. Approach: Here, we investigated the inhibitory effects of Lemon Pepper Fruit Extract (LPFE) against inflammatory biomarkers by conducting … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 14 Feb 2012 · 9,587 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro Antagonist Action of Trichoderma Strains Against Sclerotinia sclerotiorum and Sclerotium cepivorumFrancisco Daniel Hernandez Castillo, Angelica Maria Berlanga Padilla, Gabriel Gallegos Morales et al.Problem statement: Sclerotinia sclerotiorum and Sclerotium cepivorum are soil pathogens which have generated resistance to synthetic fungicides. One biological alternative to this problem is to select novel strains of Trichoderma with different mechanisms of action against these plant pathogens to ensure efficient control. Approach: In the present research was determined the antagonism effect of Mexicans Trichoderma strains on S. sclerotiorum … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 3 · 16 Aug 2011 · 9,830 accessesView PDFCite
Research ArticleOpen accessIn the abstractSynergistic Effect of Insulin on in vitro Development of Immature Bovine OocytesMojtaba Dashtizad, Abd Wahid Haron, Rosnina Yusoff et al.Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 4 · 31 Dec 2010 · 10,438 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioinhbition of Diarrhogenic Gram-negative Bacteria using the Tukey-HSD TestAdenike A.O. Ogunshe, J.O. OlaomiA variety of probiotics show promise as effective therapies in the control of diarrhoea, however, diets and geographical locations affect probiotic therapy. In this pilot study, four indigenous probiotic candidates, Lactobacillus acidophilus AAOOL4, L. reuteri AAOOCH1, L. plantarum AAOO25NN and L. delbrueckii AAOOT20 were investigated for their in vitro bactericidal effects on bacterial pathogens implicated in infantile diarrhoea using the … …American Journal of Infectious Diseases, Vol. 4, Issue 2 · 30 Jun 2008 · 7,216 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of the Immunomodulatory Properties of a Calf Lipid Thymus Extract and Standardization of a Method for Quantification of its Biological ActivityMiodrag Colic, Radmila Popovic, Dusanka Mrdakovic et al.The aim of this study was to test the immunobiological properties of a new formulation of the lipid thymus calf extract (Thymsol), prepared for in vitro cell culture experiments. At first, we showed that Thymsol enhanced the antigen specific immune response in AO rats in vitro, using Keyhole limpet hemocyanin (KLH). At lower concentrations (0.5-2.5 μg mL-1) the extract increased … …American Journal of Immunology, Vol. 3, Issue 2 · 31 Dec 2007 · 7,045 accessesView PDFCite