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135 results for “Escherichia Coli”

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Research ArticleOpen accessPhrase in the titleAn Optimized Medium for Screening of L-Asparaginase production by Escherichia coliYounes Ghasemi, Alireza Ebrahiminezhad, Sara Rasoul-Amini et al.Purified L-asparaginase II from Escherichia coli has been supplied and employed in the acute leukemia and other malignant neoplasms chemotherapy. L-asparaginase II gene (ansB) in E. coli is under regulation and certain conditions is needed for expression of this gene. In this investigation ,the various concentrations of modified M9 medium ingredients and various carbon source were tested to optimize the … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 9,698 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleHuman Insulin Modulation of Escherichia coli Adherence and ChemotaxisKarolina Klosowska, Balbina J. PlotkinEscherichia coli exhibited increased hydrophobicity and mannose-resistant epithelial cell adherence after growth in the presence of human insulin (2 μU mL-1 or 200 μUmL-1 insulin, respectively) with glucose (100 mg dL-1). Capsule production and hemagglutination were unaffected by insulin and glucose. Chemotactic attraction to glucose as compared to insulin or glucose alone was enhanced by the presence of insulin. Insulin … …American Journal of Infectious Diseases, Vol. 2, Issue 4 · 31 Dec 2006 · 7,104 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleResistance in Escherichia coli Strains Isolated from Pig Faecal Samples and Pig farm Workers, GreeceGeorge Valiakos, Alexandros Vontas, Constantina N. Tsokana et al.In this study, we compare the degree of antibiotic resistance between the E. coli strains isolated from swine faecal and farm workers. Resistance in nine widely used antibiotics was assessed by the determination of Minimum Inhibitory Concentration. Sixty-four out of 72 E. coli isolates derived from pigs and sixty out of 72 derived from farm workers showed resistance to at … …American Journal of Animal and Veterinary Sciences, Vol. 11, Issue 4 · 5 Nov 2016 · 7,505 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleESCHERICHIA COLI PATHOTYPES ASSOCIATED WITH DIARRHEA IN HUMAN AND DOMESTIC ANIMALSI.I. ShabanaRuminants are important reservoirs for zoonotic pathogenic E. coli. The objective of this study was to characterize pathogenic E. coli isolates from cattle and sheep linked to human illness with respect to their path types, serotypes and genotypes. E. coli O157:H7 isolated from cattle, sheep and human patients were compared for their genomic similarity by Pulsed-Field Gel-Electrophoresis (PFGE). PCR detection … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 3 · 2 Sep 2014 · 7,362 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleHigh-Yield Production of Bioactive Flatfish (Paralichthys olivaceus) Myostatin-1 Prodomain in Escherichia coliIl Shik Shin, Hyung-Joo Jin, Jeong Hwan Kim et al.Myostatin Propeptide (MstnPro), the N-terminal part of Mstn precursor molecule, binds to Mstn and suppress its activity. Mstn is a potent negative regulator of skeletal muscle growth in many animal species, thus MstnPro has a great potential to be used as an agent to enhance skeletal muscle growth in animal species. Bioactive fish Myostatin-1 Propeptide (Mstn1Pro) proteins were recently expressed … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 3 · 20 Sep 2012 · 8,012 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleUse of Enterobacterial Repetitive Intergenic Consensus PCR in Detecting Target(s) of Hapalindole-T, From a Cyanobacterium, in Escherichia Coli: In Silico ValidationManoj K. Tripathi, Maheep Kumar, Vinay K. Singh et al.Problem statement: Identification of newer biomolecule as well as targets are a rising concern because of increasing drug resistance in bacteria. We have isolated a broad spectrum antibiotic biomolecule Hapalindole-T from a cyanobacterium, Fisherella sp. growing on local Azadirachta indica tree bark. A model bacterium E. coli was screened spontaneously for Hap-T resistance. These resistant strains of E. coli were … …Current Research in Bioinformatics, Vol. 1, Issue 1 · 3 Mar 2012 · 13,777 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleIn Vitro Activity of Cephalexin against Community-Acquired Urinary Escherichia coli, Klebsiella and Proteus species IsolatesHumodi A. Saeed, Amira Saad Eldin MohammedProblem statement: Resistance among urinary tract pathogens to conventional antibiotics has been considered a health problem worldwide. The aim of this study was to evaluate the in vitro activity of cephalexin on urinary enterobacterial isolates. Approach: Patients with community-acquired urinary tract infections were microbiologically investigated. Urine specimens were collected from each patient. The specimens were cultured on bacteriological media. The … …American Journal of Infectious Diseases, Vol. 6, Issue 4 · 31 Dec 2010 · 8,182 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleAssessment of Site Specific Mutational Effect on Transcription Initiation at Escherichia coli PromoterS. Kannan, M. Krishnan, S. Sundarraj et al.Problem statement: It is widely accepted thought that the weak promoters control the RNA synthesis and play regulatory role in complex genetic networks in bacterial system. An experiment had been designed to address whether mutations in the -16/-17 region affect the rate of transcription at an activator-independent promoter in E. coli or not? Approach: The aim of this study was … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 2 · 30 Jun 2009 · 7,555 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleThe Development of a New, Rapid, Amperometric Immunosensor for the Detection of Low Concentrations of Bacteria Part II: Optimization of the System for Escherichia coliEric Carnes, Ebtisam WilkinsThe optimization of a flow-through immunoassay system was conducted to achieve rapid detection of low concentrations of E. coli bacteria. The system includes a new immunosensor that uses newly developed, disposable immunofiltration membranes to yield a system that is sensitive as well as simple. Comparison of the performance of this system with conventional immunoassays demonstrated a significantly shorter time for … …American Journal of Applied Sciences, Vol. 2, Issue 3 · 31 Mar 2005 · 6,780 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEpidemiology and Detection of Bacterial Diarrhea Using Multiplex Polymerase Chain Reaction, Focusing on Campylobacter spp.Eun Ju Oh, Tae Su Jang, Jung Min Park, Jae Kyung KimThis study assessed the clinical characteristics, epidemiological trends, and changes associated with major pathogens causing gastrointestinal inflammation. We retrospectively analyzed the test results of hospitalized patients who were administered a mPCR test for diarrhea causing bacteria between June 2010 and December 2019 at the Cheonan Dankook University hospital in the Republic of Korea and identified clinical characteristics and epidemiological trends … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 1 · 27 Mar 2023 · 4,979 accessesView PDFCite