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39 articles for “Clone”
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Research ArticleOpen accessIn the abstractResistance to Citrus Canker in Key/Mexican Lime Induced by β-Aminobutyric Acid and Green TeaB. Beheshti, G.R. Sharifi-Sirchi, M. Mansouri et al.Citrus bacterial canker, caused by Xanthomonas citri subsp. citri (Xcc), is a destructive disease. So far used chemicals to control this pathogen are either not effective or have harmful effects on the environment. To improve control of this disease, lime (Citrus aurantifolia) plants inoculated with Xcc were treated with β-Aminobutyric Acid (BABA), ascorbic acid (vitamin C), thiamin (vitamin B1), green … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 2 · 6 Jun 2011 · 8,191 accessesView PDFCite
Research ArticleOpen accessIn the abstractCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of the Recombinant Protein of Catechol-O-Methyltransferase from Chinese White Shrimp (Fenneropenaeus chinensis)Dian-Xiang Li, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing WangProblem statement: We have previously cloned a gene of Chinese white shrimp Catechol O-Methyltransferase (designated Fc-COMT) and characterized the gene expression pattern. In this study, expression and purification as well as activity assay of the recombinant Fc-COMT was further conducted. Approach: Using pET-30a (+) as a prokaryotic expression vector, the recombinant Fc- COMT was expressed in the supernatant of Escherichia … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,774 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Gender Determination of Ancient Human from Malay PeninsularZ.A. Shahrul Hisham, S. Sahidan, M. A.W. Rohaya et al.Problem statement: DNA samples from fourteen modern human bloods (seven males and seven females) and two ancient skeletal samples excavated from Kalumpang Island and Dungun, Peninsular Malaysia were subjected for molecular genders determination using specific primers of human AMELX and AMELY. Approach: A standard multiple PCR mixture with forward primer and either a human X-specific reverse primer for AMELX or … …American Journal of Applied Sciences, Vol. 6, Issue 10 · 31 Oct 2009 · 7,609 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhanced Survival and Nodule Occupancy of Pigeon pea Nodulating Rhizobium sp. ST1 expressing fegA Gene of Bradyrhizobium japonicum 61A152Falguni R. Joshi, Dhwani K. Desai, G. Archana, Anjana J. DesaiProblem statement: Rhizobial isolates belonging to genera (Rhizobium sp. and Mesorhizobium sp.) in our laboratory produced only catecholate type of siderophores. Although FhuA and FegA (ferrichrome receptors) homologs were found to be present in the sequenced genomes of few rhizobia (e.g., 1 in R. etli and 2 in Mesorhizobium sp. BNC1), laboratory isolates of the corresponding genera failed to utilize … …OnLine Journal of Biological Sciences, Vol. 9, Issue 2 · 30 Jun 2009 · 7,870 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssessment of Site Specific Mutational Effect on Transcription Initiation at Escherichia coli PromoterS. Kannan, M. Krishnan, S. Sundarraj et al.Problem statement: It is widely accepted thought that the weak promoters control the RNA synthesis and play regulatory role in complex genetic networks in bacterial system. An experiment had been designed to address whether mutations in the -16/-17 region affect the rate of transcription at an activator-independent promoter in E. coli or not? Approach: The aim of this study was … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 2 · 30 Jun 2009 · 7,559 accessesView PDFCite
Research ArticleOpen accessIn the abstractEpitope Mapping of Dengue-Virus-Enhancing Monoclonal-Antibody Using Phage Display Peptide LibraryChung-I Rai, Huan-Yao Lei, Yee-Shin Lin et al.The Antibody-Dependent Enhancement (ADE) hypothesis has been proposed to explain why more severe manifestations of Dengue Hemorrhagic Fever and Dengue Shock Syndrome (DHF/DSS) occur predominantly during secondary infections of Dengue Virus (DV) with different serotypes. However, the epitopes recognized by these enhancing antibodies are unclear. Recently, antipre-M monoclonal antibody (mAb 70-21), which recognized all DV serotypes without neutralizing activity, were … …American Journal of Infectious Diseases, Vol. 4, Issue 1 · 31 Mar 2008 · 8,455 accessesView PDFCite
Research ArticleOpen accessIn the abstractVibrio cholerae O1 Strains of Different Ribotypes have Similar hlyA RFLP Patterns but Different Vacuolating AbilityJorge E. Vidal, Paula Figueroa-Arredondo, Silvia Giono-Cerezo et al.Extensive cytoplasmic vacuolation on Vero and HeLa cells in vitro by the Vibrio cholerae pore forming toxin HlyA, has been previously reported by our group. Vibrio cholerae O1 and non-O1 pathogenic strains show differences in the potential to induce vacuolation, here we study occurring variations on vacuolating cytotoxic ability, related to changes in the nucleotide sequence of the hlyA-orf. A … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,989 accessesView PDFCite
Research ArticleOpen accessIn the abstractToll-Like Receptor (TLR) 2 and TLR4 Gene Expression in Canine HeartA. Linde, F. Blecha, T. MelgarejoToll-like receptors (TLRs) are archetypal pattern recognition receptors of immediate importance for an efficacious innate immune response. TLRs exhibit marked differential tissue activity and their levels within a discrete cell type can be highly dynamic. Of 13 known mammalian paralogues, three TLRs have been identified in the dog. Although cardiac TLR expression has been reported in other species, this study … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,924 accessesView PDFCite