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291 results for “Z-DNA”
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Research ArticleOpen accessEvery word in the titleEvaluation of a DNA Vaccine for Immunocontraceptive Potential Against Zona Pellucida Glycoproteins in CattleC.A. Foley, S.M. Boyle, R.E. Pearson, F.C. GwazdauskasHolstein cows were administered zona pellucida (ZP) DNA vaccine and used to determine the potential of recombinant rabbit ZP glycoproteins (rZP) as immunocontraceptive antigens. Zona pellucida proteins were purified and quantified. Cows were assigned to one of four treatment groups in which plasmids encoding rabbit ZP proteins were administered, i.d., using a gene gun (ZP55, n=2; ZP75, n=2; Hep55, n=2; … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,888 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleUltrastructural and Immunological Characterization of Hepatitis C Core Protein-DNA Plasmid ComplexesNelson Acosta-Rivero, Yaraima Aguilera, Viviana Falcon et al.Recently, it has been shown that a truncated HCV core (HCcAg) variant, covering the first 120 aa (HCcAg.120), interacts with plasmid DNA vaccine (pIDKE2), encoding the HCV structural proteins (HCcAg, E1 and E2). In the present work, HCcAg.120-pIDKE2 complexes, forming heterogeneous packaged structures, were visualized using a negative stain/rotary shadow technique. Interestingly, 72 hours after intramuscular injection of HCcAg.120-pIDKE2 complexes … …American Journal of Immunology, Vol. 2, Issue 3 · 30 Sep 2006 · 6,743 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleApplication of RNA-DNA Duplex Base Triplets to Antisense DrugsJie Yang, Yao Leng, Ju BaoSixty-four sets of three-dimensional models of RNA-DNA duplex base triplets were constructed based on codons by homology modeling method using software InsightII on Indigo workstation, which should be helpful for the study of RNA-DNA annealing, the basis of nucleic acids interactions and some peculiar motifs for design antisense oligonucleotides. Our research result reveal that the energies (such as E, Ec, … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,531 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePreliminary Study of Phylogenetic Relationship of Rice Field Chironomidae (Diptera) Inferred From DNA Sequences of Mitochondrial Cytochrome Oxidase Subunit ISalman A. Al-Shami, M.N. Siti Azizah, Che Salmah Md Rawi, Abu Hassan AhmadProblem statement: Chironomidae have been recorded in rice fields throughout the world including in many countries such as India, Australia and the USA. Although some studies provide the key to genera level and note the difficulty of identifying the larvae to species level. Chironomid researches have been hindered because of difficulties in specimen preparation, identification, morphology and literature. Systematics, phylogenetics … …American Journal of Applied Sciences, Vol. 6, Issue 5 · 31 May 2009 · 7,520 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Identification and Quantification of Marine Fish from Environmental DNA in Sulawesi Waters (Makassar Strait, Flores Sea and Bone Bay), IndonesiaNita Rukminasari, Andi Aliah Hidayani, Wilma Joanna Carolina Moka et al.The emergence of environmental DNA (eDNA) represents a recent methodological breakthrough for evaluating the presence of aquatic vertebrate species. This approach offers a relatively simple method with significant implications for conservation biology. Our study aim was to augment our understanding of marine fish biodiversity in Sulawesi waters. We employed eDNA metabarcoding to investigate fish biodiversity within Sulawesi waters, specifically focusing … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 5 Jul 2024 · 5,005 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleGenetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA LabelingRustem Saltanovich Beishov, Gulnar Sultangazina, Indira Saltanovna Beishova et al.In the article, the results of studying the gene pools of the Kazakhstan coenopopulations of a rare plant species Pulsatilla patens (L.) Mill. are shown. The biomaterial taken from five coenopopulations located in Northern Kazakhstan has been used as samples. To identify the multilocus DNA polymorphism, the authors have used ISSR (Inter Simple Sequence Repeats, or intermicrosatellite analysis) labeling. The … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 2 Apr 2021 · 5,944 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAn Effective and Viable DNA Extraction Protocol for FFPE Tissues and its Effect on Downstream Molecular ApplicationSai Sushmitha Kontham, Charles Emmanuel Jebaraj Walter, Manjula Raju et al.Archival Formalin-Fixed Paraffin-Embedded (FFPE) tissues are a readily available source of DNA for retrospective studies including genetic predisposition. FFPE tissues present significant challenges for extraction of pure genomic DNA in adequate amounts due to the harsh fixation conditions and long-term storage associated with samples. The aim of the study is to propose a simple, in-expensive, viable protocol for the extraction … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 24 Mar 2020 · 7,374 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleGenetic Characterization of Kazakh Native Sheep Breeds Using Mitochondrial DNAIlmira Mukhametzharova, Yessenbay Islamov, Saukymbek Shauyenov et al.In this study, we used mitochondrial (mt) DNA to reveal patterns of genetic diversity among six breeds of sheep native to Kazakhstan, including the Chu and Kalchengel types of Kazakh mutton-semifine-wool breed of sheep, Akzhayik, Edilbay, Kazakh fat-rumped semi-course-wool and South Kazakh Merino breeds. Mitochondrial DNA D-loop was studied in 64 samples to determine the genetic diversity of the local … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 7 Sep 2018 · 8,848 accessesView PDFCite
InvestigationOpen accessEvery word in the titleGenetic Structure of Populations of Kazakh Whiteheaded and Hereford Cattles on the Basis of Microsatellite DNAAdas Tileubekovna Tyngoziyeva, Talgat Nikolaevich Karymsakov, Serik Doldashevich NurbaevThis work is aimed at determination of genetic dissimilarities between two allied cattle breeds: Kazakh Whiteheaded and Hereford. Hair follicles were analyzed, from which DNA was extracted with subsequent amplification. Identification of amplification products was performed using an ABI Prism 310 genetic analyzer. Allele 147 was detected in Kazakh Whiteheaded cattle in locus BM 2113, there was no such allele … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 6 Dec 2017 · 7,427 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePhylogenetic of ERIC-DNA Fingerprinting and New Sequencing of Aeromonas Species and V. Cholerae DNAHawraa Natiq Kabroot AL-Fatlawy, Hawraa Abdalameer Aldahhan, Ali Hmood AlsaadiThe current study included 44 isolate of A. hydrophila, A. sobria and V. cholerae and other bacteria isolated from stool samples and environmental samples (Kufa river water and hospital environmental samples). ERIC DNA Fingerprinting with ERIC primers pairs generated distinct amplification bands ranging in size from (87 bp to 8000 kb). The 44 isolates produced 93 different patterns by ERIC … …American Journal of Applied Sciences, Vol. 14, Issue 10 · 5 May 2017 · 7,801 accessesView PDFCite