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30 results for “Hybridization”
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Research ArticleOpen accessIn the abstractA Hybrid of Fuzzy and Proportional-Integral-Derivative Controller for Electro-Hydraulic Position Servo SystemPornjit Pratumsuwan, Siripun Thongchai, Surapun TansriwongProblem statement: While classical PID controllers are sensitive to variations in the system parameters, Fuzzy controllers do not need precise information about the system variables in order to be effective. However, PID controllers are better able to control and minimize the steady state error of the system. To enhance the controller performance, hybridization of these two controller structures comes to … …Energy Research Journal, Vol. 1, Issue 2 · 31 Oct 2010 · 10,516 accessesView PDFCite
Research ArticleOpen accessIn the abstractDNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus CattleArash Javanmard, Nader Asadzadeh, Fatollah SarhadiProblem statement: Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. Approach: The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 Animals from four breeds from two species of Iranian cattle populations … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 8,024 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent CompostAzhari Samsu Baharuddin, Mohamad Nafis Abd Razak, Lim Siong Hock et al.Problems statement: Lack of information on locally isolated cellulase-producing bacterium in thermophilic compost using a mixture of Empty Fruit Bunch (EFB) and Palm Oil Mill Effluent (POME) as composting materials. Approach: The isolation of microbes from compost heap was conducted at day 7 of composting process where the mixture of composting materials consisted of 45.8% cellulose, 17.1% hemicellulose and 28.3% … …American Journal of Applied Sciences, Vol. 7, Issue 1 · 31 Jan 2010 · 12,155 accessesView PDFCite
Research ArticleOpen accessIn the abstractInduction of the Chemokines CCL3α, CCL3α and CCL5 in Atherosclerotic PatientsAlyaa Mousa, Amani Al-Zaki, Safa Taha, Moiz BakhietChemokines recruit immune cells to inflammatory sites and promote the process of inflammation. The role of these mediators in the disease process in atherosclerosis is not fully studied. The spontaneous mRNA expression and intracellular protein production of the potential inflammatory chemokines CCL3α and CCL3β (macrophage- inflammatory protein-1α and β; CCR3α/β ligand) and CCL5 (regulated upon activation, normal T cell expressed … …American Journal of Immunology, Vol. 3, Issue 2 · 31 Dec 2007 · 6,952 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Identification of Listeria monocytogenes in Processed Meat by a Combined Cultural-molecular MethodAngela Ingianni, Marta Quartuccio, Maria Antonietta Madeddu et al.The isolation and identification of Listeria monocytogenes in processed meat samples by a combined cultural-molecular method is described. It allows the identification of Listeria strains by means of a hybridization technique with a specific DNA probe directed to the listerial internalin gene. The specificity of this method was found to be 100% and sensitivity was as low as 1 CFU/2.5 … …American Journal of Infectious Diseases, Vol. 3, Issue 3 · 30 Sep 2007 · 7,252 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvidences of Apoptosis in Hepatitis C Virus-Infected Hepatocytes and Peripheral Blood Monocuclear Cells in the Absence of Liver InjuryViviana Falcón, Nelson Acosta-Rivero, Mineko Shibayama et al.Understanding the mechanism of Hepatitis C Virus (HCV) pathogenesis is an important part of HCV research. In this study, the presence of apoptosis in HCV-infected liver and Peripheral Blood Mononuclear Cells (PBMC) from patients positive for anti-HCV antibodies and negative for serum HCV-RNA was investigated. The samples obtained from 21 patients were studied by in situ Hybridization (ISH), Immunofluorescence, TUNEL … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,975 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvidences of Hepatitis C Virus Replication in Hepatocytes and Peripheral Blood Monocuclear Cells from Patients Negative for Viral RNA in SerumViviana Falcón, Nelson Acosta-Rivero, Mineko Shibayama et al.In this study, we examined the expression of hepatitis C virus (HCV) components in hepatocytes and peripheral blood mononuclear cells (PBMC) from patients positive for anti-HCV antibodies and negative for serum HCV-RNA. The samples obtained from 25 randomly selected patients (13 of 25 patients showed no histological evidences of chronic hepatitis and had normal serum ALAT and GGTP levels) were … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 7,164 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Transcript Labeling Techniques and Development of a Membrane-based Parallel Gene Expression AssayMark A. Fisher, Bonnie B. Plikaytis, Thomas M. ShinnickAn inexpensive alternative to micro arrays was developed to examine the expression of small sets of genes in parallel and used to compare direct and indirect transcript labeling methods. Psoralen-biotin direct labeling was ~10-fold less sensitive than reverse transcriptase-biotin-dUTP labeling, but the enzymatic method generated higher levels of background noise in miniarray hybridizations. The reproducibility of hybridization intensities was the … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,868 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Sequence Based Validation of Gene Expression Microarray DataGerhard G. Thallinger, Eva Obermayr, Pornpimol Charoentong et al.Problem statement: Quantitative Reverse Transcription PCR (RT-qPCR) is often used to validate microarray data. Previous studies show different levels of correlation, without further investigation of influencing factors. Approach: We compared expression levels of 381 genes obtained from microarray hybridizations and from TaqMan based RT-qPCR assays. Correlation of expression levels was determined by comparing: (i) single genes across samples, (ii) all … …Current Research in Bioinformatics, Vol. 1, Issue 1 · 19 Jan 2012 · 13,715 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Widely Used Diagnostics "DNA Microarray"-A ReviewR. Manoj KumarProblem statement: DNA microarray technique is one of the latest advances in the field of molecular biology and medicine. It is a multiplex technique used in combination of bioinformatics and statistical data analysis. Since, 1995, the technique offers the possibility of conducting tens or hundreds of thousands of simultaneous hybridizations. Approach: This increased experimental efficiency permits high throughput and whole … …American Journal of Infectious Diseases, Vol. 5, Issue 3 · 30 Sep 2009 · 7,787 accessesView PDFCite