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35 results for “Expression Vector”
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Research ArticleOpen accessIn the abstractEfficient Classification of Cancer using Support Vector Machines and Modified Extreme Learning Machine based on Analysis of Variance FeaturesA. Bharathi, A.M. NatarajanProblem statement: The primary objective is to propose efficient cancer classification techniques which provide reliable and significant classification accuracy. To achieve this primary research goal is to find the smallest set of genes that can ensure high accuracy in classification using supervised machine learning algorithms. The significance of finding the minimum subset is three fold: (a) The computational burden and … …American Journal of Applied Sciences, Vol. 8, Issue 12 · 22 Oct 2011 · 7,441 accessesView PDFCite
Research ArticleOpen accessIn the abstractCancer Classification using Support Vector Machines and Relevance Vector Machine based on Analysis of Variance FeaturesA. Bharathi, A.M. NatarajanProblem statement: The objective of this study is, to find the smallest set of genes that can ensure highly accurate classification of cancer from micro array data by using supervised machine learning algorithms. The significance of finding the minimum subset is three fold: The computational burden and noise arising from irrelevant genes are much reduced; the cost for cancer testing … …Journal of Computer Science, Vol. 7, Issue 9 · 25 Jul 2011 · 7,593 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of the Recombinant Protein of Catechol-O-Methyltransferase from Chinese White Shrimp (Fenneropenaeus chinensis)Dian-Xiang Li, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing WangProblem statement: We have previously cloned a gene of Chinese white shrimp Catechol O-Methyltransferase (designated Fc-COMT) and characterized the gene expression pattern. In this study, expression and purification as well as activity assay of the recombinant Fc-COMT was further conducted. Approach: Using pET-30a (+) as a prokaryotic expression vector, the recombinant Fc- COMT was expressed in the supernatant of Escherichia … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,770 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhanced Survival and Nodule Occupancy of Pigeon pea Nodulating Rhizobium sp. ST1 expressing fegA Gene of Bradyrhizobium japonicum 61A152Falguni R. Joshi, Dhwani K. Desai, G. Archana, Anjana J. DesaiProblem statement: Rhizobial isolates belonging to genera (Rhizobium sp. and Mesorhizobium sp.) in our laboratory produced only catecholate type of siderophores. Although FhuA and FegA (ferrichrome receptors) homologs were found to be present in the sequenced genomes of few rhizobia (e.g., 1 in R. etli and 2 in Mesorhizobium sp. BNC1), laboratory isolates of the corresponding genera failed to utilize … …OnLine Journal of Biological Sciences, Vol. 9, Issue 2 · 30 Jun 2009 · 7,865 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssessment of Site Specific Mutational Effect on Transcription Initiation at Escherichia coli PromoterS. Kannan, M. Krishnan, S. Sundarraj et al.Problem statement: It is widely accepted thought that the weak promoters control the RNA synthesis and play regulatory role in complex genetic networks in bacterial system. An experiment had been designed to address whether mutations in the -16/-17 region affect the rate of transcription at an activator-independent promoter in E. coli or not? Approach: The aim of this study was … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 2 · 30 Jun 2009 · 7,555 accessesView PDFCite
Research ArticleOpen accessIn the abstractAAV-Based Targeting Gene TherapyWenfang Shi, Mingxia Yu, Qijun QianSince the first parvovirus serotype AAV2 was isolated from human and used as a vector for gene therapy application, there have been significant progresses in AAV vector development. AAV vectors have been extensively investigated in gene therapy for a broad application. AAV vectors have been considered as the first choice of vector due to efficient infectivity, stable expression and nonpathogenicity. … …American Journal of Immunology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,960 accessesView PDFCite
Research ArticleOpen accessIn the abstractAn Inducible System for the Identification of Target Genes for a Regulator in MycobacteriaShruti Jain, Garima Khare, Pushplata Tripathi, Anil K. TyagiWe described principle and application of an inducible system for identification of target genes of a given mycobacterial protein with a regulatory function. This vector system, a promoterprobe vector, carries (i) promoterless lacZ as the reporter gene and (ii) the mycobacterial gene encoding the regulatory protein under the transcriptional control of the promoter of highly inducible mycobacterial gene encoding acetamidase. … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 7,249 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Soybean Mosaic Virus NIa Protein and its Processing Event in Bacterial ExpressionBong K. Choi, Jae S. Park, Hye J. Ahn et al.Soybean mosaic virus (SMV)-CN18 is an Rsv resistance-breaking (RB) isolate to overcome soybean resistance genes Rsv1, Rsv3 and Rsv4. The aim of this study was to characterize nuclear inclusion protein a (NIa protein) of RB isolate at the molecular level and demonstrate its processing into genome-linked protein (VPg) and NIa-Pro domains in Esherichia coli containing a bacterial expression pET vector … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,902 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of Two New Recombinant Antagonists of Fibrinogen ReceptorJianbo Yang, Jia Yao, Kun Yang et al.The gene sequence of Decorsin which is extracted from a kind of North American leeches was synthesized. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the carboxyl terminal 27 amino acid residues of Decorsin(AnnV-D27), were constructed. And a 10 amino acids linker peptide of GGGGSGGGGS was inserted between Annexin V and Decorsin in AnnV-D39. Using pET-28(a+) … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,913 accessesView PDFCite
Research ArticleOpen accessIn the abstractInduced Production of α-Tocotrienol by Co-Suppression of Tocopherol Cyclase Gene in Vegetable CropsUmaiyal Munusamy, Siti Nor Akmar Abdullah, Maheran Abdul Aziz et al.This study involves the induced production of α-tocotrienol by the co-suppression of tocopherol cyclase gene. The Tocopherol/Tocotrienol Cyclase (TC) is a key enzyme involved in the biosynthesis of α-tocotrienol. The partial conserved cDNA of TC gene isolated from the oil palm (Elaeis guineensis) that shared greater than 75% sequence identity with TC sequences from other plant species was used to … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 7,706 accessesView PDFCite