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26 results for “E. coli expression”
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Research ArticleOpen accessIn the abstractInduction of the Chemokines CCL3α, CCL3α and CCL5 in Atherosclerotic PatientsAlyaa Mousa, Amani Al-Zaki, Safa Taha, Moiz BakhietChemokines recruit immune cells to inflammatory sites and promote the process of inflammation. The role of these mediators in the disease process in atherosclerosis is not fully studied. The spontaneous mRNA expression and intracellular protein production of the potential inflammatory chemokines CCL3α and CCL3β (macrophage- inflammatory protein-1α and β; CCR3α/β ligand) and CCL5 (regulated upon activation, normal T cell expressed … …American Journal of Immunology, Vol. 3, Issue 2 · 31 Dec 2007 · 6,952 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression of Thioredoxin-Fusion Proteins of α-Gliadin, γ-Gliadin and Low Molecular Weight Glutenin, from Wheat Endosperm and their Domains in EnterobacteriaClaudia G. Benitez-Cardoza, Yves Popineau, Jacques GueguenWheat seed storage proteins play a determining role in the viscoelastic properties of wheat gluten. The genes encoding α-gliadin, γ-gliadin and Low Molecular Weight glutenin and their N-central-repetitive and C-terminal domains from wheat endosperm had been subcloned into a thioredoxin expression system (pET102/D-Topo) and produced as fusion proteins in E. coli. The expression levels for each of the proteins varied … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,746 accessesView PDFCite
Research ArticleOpen accessIn the abstractHuman Insulin Modulation of Escherichia coli Adherence and ChemotaxisKarolina Klosowska, Balbina J. PlotkinEscherichia coli exhibited increased hydrophobicity and mannose-resistant epithelial cell adherence after growth in the presence of human insulin (2 μU mL-1 or 200 μUmL-1 insulin, respectively) with glucose (100 mg dL-1). Capsule production and hemagglutination were unaffected by insulin and glucose. Chemotactic attraction to glucose as compared to insulin or glucose alone was enhanced by the presence of insulin. Insulin … …American Journal of Infectious Diseases, Vol. 2, Issue 4 · 31 Dec 2006 · 7,104 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Murine Charged Multivesicular Body Protein 2A, CHMP2A Interacts with the 5' and 3' Terminal Regions of Dengue Virus Complementary Minus-strand RNASuchita Chaudhry, Sathyamangalam Swaminathan, Navin KhannaDengue (DEN) viruses, of which there are four distinct serotypes (DEN-1, -2, -3 and –4), belong to the Flaviviridae family. Their ‘plus’ sense RNA genomes contain Non-Translated Regions (NTRs) at their 5’ and 3’ ends. Replication of viral genomic RNA, which takes place in close association with host cell membranes, involves the initial synthesis of the complementary minus sense RNA … …American Journal of Infectious Diseases, Vol. 2, Issue 1 · 31 Mar 2006 · 7,314 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of Two New Recombinant Antagonists of Fibrinogen ReceptorJianbo Yang, Jia Yao, Kun Yang et al.The gene sequence of Decorsin which is extracted from a kind of North American leeches was synthesized. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the carboxyl terminal 27 amino acid residues of Decorsin(AnnV-D27), were constructed. And a 10 amino acids linker peptide of GGGGSGGGGS was inserted between Annexin V and Decorsin in AnnV-D39. Using pET-28(a+) … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,913 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordHeterologous Expression and Characterization of Thermostable Levansucrase (BsSacB) from Bacillus subtilis BB03Viniti Vaidya, Gajjeraman Prabu, D. Theertha PrasadThermostable levansucrase encoding gene (sacB) with its promoter was isolated from Bacillus subtilis BB03. BsSacB is composed of 1419 bp, encoding 473 amino acid residues and with promoter regions TTGCAA (-35) and TAGAAT (-10). The amino acid sequence analysis of BsSacB showed conserved motifs of microbial fructosyltransferase belonging to Glycoside Hydrolase family 68 (GH68). Comparative analysis of the protein structure … …OnLine Journal of Biological Sciences, Vol. 15, Issue 1 · 25 Mar 2015 · 7,371 accessesView PDFCite