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51 articles for “3C protease”
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Research ArticleOpen accessIn the keywordsPapain, a Plant Enzyme of Biological Importance: A ReviewFlorence Mamboya, Ezekiel AmriPapain is a plant proteolytic enzyme for the cysteine proteinase family cysteine protease enzyme in which enormous progress has been made to understand its functions. Papain is found naturally in papaya (Carica papaya L.) manufactured from the latex of raw papaya fruits. The enzyme is able to break down organic molecules made of amino acids, known as polypeptides and thus … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 44,698 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPurification of Chymotrypsin-Trypsin Inhibitor from Winged Bean Seeds using Single Step Immunoaffinity ColumnSanhita Roy, Samir Kumar DuttaProblem statement: Winged bean is a rich source of protease inhibitors. The seeds of winged bean contain several serine protease inhibitors of which winged bean chymotrypsin-trypsin inhibitor (WbCTI) is of significant importance due to its dual inhibition of both chymotrypsin and trypsin. The purification of WbCTI has always been difficult and a time taking process that involved large number of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 7,095 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCharacterization and Wash Performance Analysis of Microbial Extracellular Enzymes from East Calcutta Wetland in IndiaRamesh Malathu, Sanhita Chowdhury, Madhusmita Mishra et al.Extracellular protease from a novel bacterial isolate showing maximum similarity of 98.22% with Microbacterium luteolum was obtained from East Calcutta Wetland, India. It showed compatibility with commercial detergents. The enzyme retains more than 60% of its activity between 6.0 to 10.5 pH. The maximum activity is at pH 7.5 with 71% activity at pH 10.0 and 10.5. The protease retained … …American Journal of Applied Sciences, Vol. 5, Issue 12 · 31 Dec 2008 · 8,727 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Proteolytic System of Candida dubliniensisLoaiza-Loeza Salomé, Parra-Ortega Berenice, Bautista-Muñoz Consuelo et al.Proteases of Candida dubliniensis have been scarcely studied, these enzymes may play an important role in nitrogen metabolism, post-translational processing, nutritional stress, dimorphism, virulence, etc. In this work, we report the presence of five different intracellular proteases and one extracellular proteolytic activity. The intracellular proteases are: aminopeptidase ycdAPE, carboxypeptidase ycdCP, dipeptidyl aminopeptidase ycdDAP, proteinases ycdPrA and ycdPrB, and extracellular protease … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,953 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInduction of Caspase-dependant Apoptosis by Enterovirus 70 in Human Lens Epithelial SRA01/04 CellsDequan Chen, Chris Duggan, Donald E. Texada et al.Enterovirus 70 (EV70), an etiological agent of acute hemorrhagic conjunctivitis (AHC), induces apoptosis in human Chang’s conjunctival (HCC) cells. EV70 is also able to infect and replicate in some other human cells such as human lens epithelial SRA01/04 cells and U937 cells. EV70 infection was found to induce apoptosis in SRA01/04 cells. During EV70-induced apoptosis of SRA01/04 cells, the expression … …American Journal of Infectious Diseases, Vol. 1, Issue 2 · 30 Jun 2005 · 6,636 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Sturgeon Protein Fractions on Active Peptide Generation via Enzymatic HydrolysisMengjie Lu, Yingmei Hou, Yejing Sun et al.To identify the sturgeon protein component responsible for generating bioactive peptides, including antioxidant peptides and alcohol dehydrogenase (ADH) promoting peptides, sturgeon protein and its isolated protein components (sarcoplasmic, myofibrillar, and stromal proteins) were studied. Among the four isolated protein components, stromal protein demonstrated the highest susceptibility to enzymatic hydrolysis (P<0.05). Besides, pepsin and alkaline protease are the most effective enzymes … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 1 · 8 Apr 2026 · 1,419 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effect of Different Functional Microbial Agents on the Biological Characteristics and Rhizosphere Enzyme Activity of Hydroponic CucumberCe Wang, Ouya Zhao, Shiyou Sun et al.This study aims to comprehensively explore the effect of functional microbial agents on healthy soil cultivation and attempt to increase cucumber yield. Three different microbial agents were used for cucumber seedlings by virtue of the hydroponic cultivation method in order to study their effects on enzyme activity and seedling growth and reveal the relationship between rhizosphere enzyme activity and carbon, … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 24 Mar 2025 · 5,510 accessesView PDFCite
Research ArticleOpen accessIn the abstractMaximizing Fungal Growth in State-of-the-Art Tray Solid-State Fermenter: Aeration Strategies Optimized for Enhanced PerformanceMusaalbakri Abdul Manan, Colin WebbThis study introduced a tray fermenter, featuring eight layers of squared perforated trays, specifically designed for solid-state fermentation applications. It is named the multi-layer squared tray solid-state fermenter. The fermenter employs two aeration strategies with varying air flow rates to optimize fungal growth. Firstly, sterilized moistened air at 8 L/min flows from the bottom through the perforated trays. Secondly, a … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 9 Apr 2024 · 6,453 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro Ruminal Cumulative Gas and Methane Production, Enzyme Activity, Fermentation Profile and Nutrient Digestibility on Feed Supplemented with Organic SeleniumMoh. Sofi’ul Anam, Andriyani Astuti, Budi Prasetyo Widyobroto et al.Methane (CH4) emissions are indeed a global environmental concern and ruminants are a significant source of these emissions; however, there is limited understanding regarding the contribution of organic Selenium (Se) in mitigating CH4 emissions. Hence, this study investigated the impact of supplementing organic Se from Selenomethionine (Se-Met) on the in vitro ruminal cumulative gas and CH4 production, fermentation profile, enzyme … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 26 Oct 2023 · 5,215 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Humic Acid Dosage and Fermentation Time with Bacillus subtilis on Nutrient Content and Quality of Palm Kernel CakeMirnawati, Gita Ciptaan, Yoga Seftiadi, Malik MakmurThis study aims to determine the effects of humic acid dosage and fermentation time with Bacillus subtilis on the nutrient content and quality of Palm Kernel Cake (PKC). A Completely Randomized Design (CRD) with a 3 × 4 factorial pattern and 3 replications was applied. The present experiment considered two major parameters, termed A and B. Factor A represents the … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 1 · 15 Feb 2022 · 6,699 accessesView PDFCite