91 results for “sequencing”

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Review ArticleOpen accessIn the keywordsComparative Study on Microbial Communities of Soil, Grape Must and Wine Fermentation of Cabernet Sauvignon Inoculated with three Commercial Yeast of Saccharomyces cerevisiaeRongbin Li, Xu Shi, Yaqiong Liu et al.This study examined the microbial diversity in soil, grape must and wine fermentation of Cabernet Sauvignon inoculated with three different starters (S01: Xinjiang Saccharomyces cerevisiae CEC01; SCA: Ningxia Saccharomyces cerevisiae CECA; S96: Saccharomyces cerevisiae AWRI 796) using a high-throughput sequencing technique. The results showed that 227 bacterial genera and 20 fungi genera were shared in all samples, consisting of Saccharomyces, … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 4 · 24 Dec 2021 · 5,997 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsMycobacterium tuberculosis Complex Mutations in Drug Resistant Clinical Isolates from Southwest MexicoMartínez-Cruz Perla Mónica, Nakamura-López Yuko, Quintero-Hernández Verónica et al.Mutations in target genes have been described in Mycobacterium tuberculosis Complex (MTBc) drug resistant isolates worldwide. In Mexico, not enough information has been reported in this concern. The aim of this study was to characterize mutations related to resistance to first line drugs in MTBc isolates from Oaxaca, Mexico. MTBc isolates were identified in clinical samples from Tuberculosis (TB) patients. … …American Journal of Infectious Diseases, Vol. 17, Issue 4 · 28 Oct 2021 · 6,667 accessesView PDF Cite

Review ArticleOpen accessIn the keywordsCOVID-19 Reinfection and its Diagnostic ApproachAshish William, Ravinder KaurCOVID-19 caused by Coronavirus strain “Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-COV-2)” has been responsible for the significant threat to the world and its pandemic has become the most important matter of concern in health care systems. The cases of reinfection with virus have been noted in the patients after the discharge. The lack of reporting of COVID-19 virus in … …American Journal of Applied Sciences, Vol. 18, Issue 1 · 3 Apr 2021 · 7,880 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsAn Effective and Viable DNA Extraction Protocol for FFPE Tissues and its Effect on Downstream Molecular ApplicationSai Sushmitha Kontham, Charles Emmanuel Jebaraj Walter, Manjula Raju et al.Archival Formalin-Fixed Paraffin-Embedded (FFPE) tissues are a readily available source of DNA for retrospective studies including genetic predisposition. FFPE tissues present significant challenges for extraction of pure genomic DNA in adequate amounts due to the harsh fixation conditions and long-term storage associated with samples. The aim of the study is to propose a simple, in-expensive, viable protocol for the extraction … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 24 Mar 2020 · 7,374 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsGenetic Mutation Assessment of NPM1 Gene and Gene Expression of MDR1 in Iraqi Patients with Acute Myeloid LeukemiaAhmed Abduljabbar Suleiman, Ali Zaid Al-Saffar, Tamadher Abbas Rafaa, Hasan Abdulwahab JwadSince its discovery, mutations in NPM1 have been frequently associated with a large number of Acute Myeloid Leukemia (AML) patients. The vast majority of genetic changes were previously detected in exon 12, however little information report mutations in Nuclear Export Signal region (NES) of NPM1. Sequencing analysis included exon 2 and 3 for 75 Iraqi AML patients showed three SNPs, … …OnLine Journal of Biological Sciences, Vol. 18, Issue 1 · 11 Jan 2018 · 7,782 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsThe Investigation of Organelle Genomes of Extra Nuclear Sunflower Mutants with Variegated PhenotypeIrina Dremuk, Nikolay Kozel, Mariya Logacheva et al.The comparative analysis of complete chloroplast and mitochondrial DNA sequences of the original inbred line 3629 and three extra nuclear mutant lines variegated-1, variegated-15, variegated-17, obtained by the N-nitroso-N-methylurea induced mutagenesis, was done. The studied mutant lines were presented two different phenotypes with diverse chlorophyll content: Pale/green mutant line variegated-17 with 1.8% relative (to 3629 line) chlorophyll content in mutant … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 24 Oct 2017 · 7,633 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsClosing the Gaps in Rat Cytomegalovirus ALL-03 (Malaysian Strain) Genomic ScaffoldKrishnan Nair Balakrishnan, Ashwaq Ahmed Abdullah, Yusuf Abba et al.Next generation sequencing technologies has revolutionized genomic research by producing a large volume of sequence data and lowest per base cost compared to the traditional sanger method. Although this technology offers many advantages, gap occurrences are commonly found in draft assemblies. The same problem was observed with Rat Cytomegalovirus (RCMV) ALL-03 (Malaysia strain), where a complete genome sequence could not … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 3 · 8 Jun 2015 · 7,578 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsDiversity of the Rhizosphere Soil Culture-Dependent Fungi of Mature TobaccoXiaolin Li, Xinzhu Li, Kewei Zhao et al.Problem statement: The maturation of tobacco is a very important period of tobacco production. Until recently, there are no many reports on tobacco rhizospheric culturable fungi, especially at the mature stage. Approach: Five rhizosphere soils of tobacco and five non-rhizosphere soils of tobacco were collected in Yanbian county, Panzhihua. Sixty-one fungal strains isolated from these soil samples were analyzed by … …Current Research in Microbiology, Vol. 2, Issue 1 · 26 Oct 2011 · 9,880 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsMolecular Characterization of a Phytoplasma Associated with Coconut Yellow Decline (CYD) in MalaysiaN. Nejat, K. Sijam, S. N.A. Abdullah et al.Problem statement: Molecular methods have been used to detect phytoplasma in Malayan Red Dwarf (MRD) ecotype of coconut for the first time in Malaysia. Detect phytoplasma from coconut showing yellowing symptoms by nested PCR and Classify phytoplasma associated with disease of coconut palms, based on analysis of 16S rRNA gene operon sequences and virtual RFLP. Approach: Twenty MRD symptomatic palms … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 7,919 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsA Neural Network for Generating Adaptive LessonsHassina Seridi-Bouchelaghem, Toufik Sari, Mokhtar SellamiTraditional sequencing technology developed in the field of intelligent tutoring systems have not find an immediate place in large-scale Web-based education. This study investigates the use of computational intelligence for adaptive lesson generation in a distance learning environment over the Web. An approach for adaptive pedagogical hypermedia document generation is proposed and implemented in a prototype called KnowledgeClass. This approach … …Journal of Computer Science, Vol. 1, Issue 2 · 30 Jun 2005 · 7,269 accessesView PDF Cite