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32 articles for “quantitative PCR”
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Research ArticleOpen accessIn the abstractClinical Significance of Precise Hyperthermic Intraperitoneal Chemotherapy Up-regulation RP11-356I2.2 Restraining the Occurrence and Development of Gastric CancerYu Yan, Feng Yanlin, Feng Jinxin et al.In order to study the therapeutic effect and possible mechanism of Precise Hyperthermic Intraperitoneal Chemotherapy (HIPEC) on gastric cancer, the expression of RP11-356I2.2 in gastric cancer cell lines and tissues was detected by real-time fluorescence quantitative PCR (RT-PCR). Chi-square test was used to analyze the correlation between the expression and clinic pathological parameters. Western blotting was used to detect the … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 4 Jun 2020 · 6,487 accessesView PDFCite
Research ArticleOpen accessIn the abstractMorpho-Physiological and Genetic Responses of Bali Local Rice Cultivars to Drought Stress at Seedling StageMade Pharmawati, Luh Putu WrasiatiRice is the main food source in many countries in the world. One of the major limiting factors for rice growth and productivity is drought stress. In Bali there are several local rice cultivars, two of them are ‘Mansur' and ‘Putih Cempaka'. The cultivar ‘IR 64' is one rice cultivar released by IRRI. The responses of those rice cultivars to … …OnLine Journal of Biological Sciences, Vol. 18, Issue 1 · 29 Mar 2018 · 7,644 accessesView PDFCite
Research ArticleOpen accessIn the abstractRelative Transcription Expression Level of SIRT1, SIRT2 and SIRT3 in Correlation to the Expression of a Set of Selected Cancer Related Genes in Human Breast CancerHamdy Swelim, Omnia Mansour, Salah Abdel-Rahman et al.Mammalian Sirtuins have been shown to perform distinct cellular functions and deregulated expression of these genes was reported to be involved in the development of various malignancies including breast cancer. An increasing number of evidence indicates that Sirtuins have both tumor promoter and tumor suppressor functions. However, the roles of Sirtuins have not been well-studied in breast cancer. In the … …OnLine Journal of Biological Sciences, Vol. 18, Issue 2 · 18 Jan 2018 · 7,476 accessesView PDFCite
Research ArticleOpen accessIn the abstractAge-Dependent Variation of Telomere Length and DNA Damage in ChickenMaxim S. Makarenko, Alexander V. Usatov, Lyubov V. Getmantseva et al.Birds are unique models for molecular studies on aging processes and cellular senescence because of their slow rates of aging and comparatively long life spans among homoeothermic animals. The study aimed to examine the relative telomere length and amount of nuclear, mitochondrial DNA lesions in hens of different ages and to find out the correlation between these biomarkers of aging. … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 14 Dec 2017 · 7,505 accessesView PDFCite
Research ArticleOpen accessIn the abstractWhat is Missing in STD Screening in Hong Kong?Clement Leung-Kwok Chan, Wai Yee Chan, Christopher Hon Ki Cheng, Ping XiaThis retrospective analysis was to study the prevalence of Sexually Transmitted Diseases (STD) in a Reproductive Medical Center in Hong Kong. A total of 1190 patients were included in this study. Group 1A, couples had no symptoms but presented with subinfertility; Group 1B, the subfertile couples were positive for either Chlamydia trachomatis (CT) or Ureaplasma urealyticum (UU); Group 2, couples … …Current Research in Medicine, Vol. 7, Issue 2 · 11 Jan 2017 · 8,407 accessesView PDFCite
Research ArticleOpen accessIn the abstractGene Expression Profiling of Toll-Like Receptor 4 and 5 in Peripheral Blood Mononuclear Cells of Patients with Systemic SclerosisSimin Almasi, Saeed Aslani, Hadi Poormoghim et al.The Toll-Like Receptor (TLR) family is appeared to be expressed in many cell types in the immune system and plays a role in the pathogenesis of various autoimmune diseases. The expression profile and role of TLRs in Systemic Sclerosis (SSc) have been partly explained. It is aimed through this investigation to evaluate the expression pattern of TLR 4 and 5 … …American Journal of Immunology, Vol. 12, Issue 1 · 17 Mar 2016 · 7,049 accessesView PDFCite
Research ArticleOpen accessIn the abstractImpact of Acute Bronchiolitis on Cardiac Functions and Serum microRNA-122 and 499Mona Abd El-Gelel Mohamed, Khalid Mahmoud ZayedBronchiolitis is an acute inflammatory injury of the bronchioles that is usually caused by a viral infection, most commonly Respiratory Syncytial Virus (RSV). This study aimed to verify the direct injurious effect of acute bronchiolitis on the myocardium and serum level of microRNA-122 and 499 (miR-122 and miR-499) of infected infants. Serum lactate dehydrogenase (LDH), creatine phosphokinase-isoenzyme MB (CK-MB) and … …American Journal of Infectious Diseases, Vol. 12, Issue 1 · 26 Jan 2016 · 7,250 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenomic Signature of Respiratory Microbes on Human Scalp EpitheliaBhola Nath Paul, Vani Mishra, Jasmeet Kaur Chhabra, Divyani PaulDespite overlap between habitats occupied by diverse Airborne Respiratory Microbes (ARM), the airway epithelium is known for respiratory microbe infections, human scalp epithelium is undocumented. Analogous to airways epithelium, the scalp epithelium is vulnerable to infection by dormant variants of sensitive microbes that underlie many chronic and relapsing diseases, difficult to eradicate by conventional antibiotics and challenged our ability to … …American Journal of Immunology, Vol. 11, Issue 3 · 11 Sep 2015 · 7,074 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Algorithm for Variable and Samples Selection in Multivariate Calibration ProblemsKelton de Souza Santiago, Anderson Silva Soares, Telma Woerle de Lima et al.One of the main problems of quantitative analytical chemistry is to estimate the concentration of one or more species from the values of certain physicochemical properties of the system of interest. For this it is necessary to construct a calibration model, i.e., to determine the relationship between measured properties and concentrations. The multivariate calibration is one of the most successful … …Journal of Computer Science, Vol. 11, Issue 4 · 8 Jun 2015 · 7,381 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Different Drugs in Down-Regulation of Efflux Pump Genes expression in Methicillin-Resistant Staphylococcus aureus StrainsManu Chaudhary, Santosh Kumar Patnaik, Anurag PayasiAntimicrobial resistance caused by efflux systems is not widely studied in Staphylococcus aureus. In the present study, prevalence of NorA and NorB efflux pumps was studied in thirteen Methicillin-Resistant Staphylococcus aureus (MRSA) strains using a pair of primers with Polymerase Chain Reaction (PCR). Antibiotic susceptibility study in NorA and NorB efflux positive isolates was carried out according to the Clinical … …American Journal of Infectious Diseases, Vol. 10, Issue 4 · 7 Jan 2015 · 7,451 accessesView PDFCite