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33 articles for “purity”
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Research ArticleOpen accessIn the abstractInfluence of Tags on the Binding Affinity of Acyl-CoA Binding ProteinJizhong Han, Yunlong Sun, Yu Chen et al.Tagged fusion proteins are frequently employed for protein purification methods, but their effects on protein function and binding affinity are rarely studied. Here we expressed recombinant protein Acyl-CoA Binding Protein (ACBP) cloned from the full-length cDNA of Aspergillus oryzae and Saccharomyces cerevisiae. ACBP was expressed in Escherichia coli fused to a Maltose-Binding Protein (MBP) and Histidine-tag fusion. Recombinant ACBP was … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 23 Jan 2019 · 9,097 accessesView PDFCite
Research ArticleOpen accessIn the abstractLarvicidal Activity of Bruceine A against Aedes aegypti and Toxicity on Vero CellsDwi Sutiningsih, Mustofa, Tri Baskoro Tunggul Satoto, Edhi MartonoVector control is still based on the use of chemical insecticides, which can cause death of nontarget animals, pollution and the emergence of vector resistance. This study aims to assess the larvicidal activity of bruceine A against larvae of Aedes aegypti and its cytotoxic activity against Vero cells. Extraction and isolation of bruceine A from the seeds of Brucea javanica … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 4 Sep 2018 · 7,912 accessesView PDFCite
InvestigationOpen accessIn the abstractThe Influence of Bioorganic Preparations and Mineral Fertilizers to the Productivity and Quality of Beetroot in the Subsurface Irrigation in the South-East of KazakhstanTemirzhan Yerkassovich Aitbayev, Bekzat Abiyevich Turegeldiyev, Laura Abdireevna Buribayeva et al.Vegetables are valuable sources of vitamins, organic acids and mineral salts. In Kazakhstan, the consumption rate of vegetables per person is 120 kg per year, where 8 kg is beetroot. Beetroot cultivated area is 7,000 hectares. The yield is 150,000 tons, and the demand is 144,000 tons. Beetroot is one of the most popular and all year round vegetable that … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 10 Jan 2018 · 7,564 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Simulation Study of Enhanced Distillation Using Ultrasound Waves for the Separation of Azeotropic MixturesTaha Mahdi, Ammar Emad Al-KawazThe potential of ultrasound waves to enhance distillation system for azeotropic mixture is examined through process flowsheeting using Aspen Plus. Since sonication is not a standard feature in the software library, a mathematical model of the proposed distillation system is developed based on first principles in Aspen Custom Modeler and later exported to Aspen Plus environment for process simulation and … …American Journal of Engineering and Applied Sciences, Vol. 11, Issue 1 · 2 Jan 2018 · 8,411 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization of Raw Materials for Preparation of Transparent Soil Based on Analytic Hierarchy ProcessJianjun Liu, Yao Wang, Rui Song, Min YeThe application of man-made transparent soil has greatly contributed to the development of the visual research in physical model test. Comparing to the traditional model test, the transparent soil has advantages with respect to transparency, visualization, lower cost and utility et al. However, the transparency of material itself seriously restricted the effect of the results during the model text. This … …American Journal of Engineering and Applied Sciences, Vol. 10, Issue 1 · 1 Mar 2017 · 7,266 accessesView PDFCite
Research ArticleOpen accessIn the abstractGraphene Synthesis by Plasma-Enhanced CVD Growth with EthanolTeresa Campo, María Cotto, Francisco Márquez et al.A modified route to synthesize graphene flakes is proposed using the Chemical Vapor Deposition (CVD) technique, by using copper substrates as supports. The carbon source used was ethanol, the synthesis temperature was 950°C and the pressure was controlled along the whole process. In this CVD synthesis process the incorporation of the carbon source was produced at low pressure and 950°C … …American Journal of Engineering and Applied Sciences, Vol. 9, Issue 3 · 27 Jul 2016 · 9,146 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Bioactivity of Fucoxanthin from Undaria pinnatifida in VitroYiting Li, Ye Liu, Yuepeng Wang et al.Fucoxanthin, a marine carotenoid, can be found in marine algae and some marine invertebrates, has remarkable biological properties for human health. It is known that fucoxanthin has anti-cancer activity on some human cancer cells such as human colon cancers, leukemia cells, prostate cancer cells, breast cancer cells. In this study, the fucoxanthin was extracted from Undaria pinnatifida, after purification by … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 2 · 7 Jun 2016 · 7,866 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Clean-Green Synthesis of Platinum Nanoparticles Utilizing a Pernicious Weed Lantana (Lantana Camara)Musthafa O. Mavukkandy, Sudip Chakraborty, Tasneem Abbasi, Shahid A. AbbasiA rapid, reproducible single step method is proposed for synthesizing platinum nanoparticles using an invasive weed lantana (Lantana camara, L). The method involves mixing of platinum (VI) solution, lantana leaf extract and ascorbic acid in appropriate concentrations and keeping the mixture at 95°C for 8 min. The collective action of ascorbic acid and leaf extract reduced the chloroplatinic acid to … …American Journal of Engineering and Applied Sciences, Vol. 9, Issue 1 · 23 Feb 2016 · 10,447 accessesView PDFCite
Research NotesOpen accessIn the abstractPolymerisation and Oxidation of Docosahexaenoic AcidBrian Mundell Ross, Shelly Lynn Browning, Adrian McKeeOils rich in polyunsaturated fatty acids, such as linseed oil, can oxidise to form polymers. These can be used as protective coatings and act as an alternative to those derived from petrochemicals. Such renewable source oils usually require the addition of metal ions as catalysts of the polymerisation process and/or the partial pre-oxidation of the oil before use. We hypothesised … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 11 Dec 2015 · 7,612 accessesView PDFCite
Research ArticleOpen accessIn the abstractIntracellular L-Asparaginase from Bacillus sp. PG02: Purification, Biochemical Characterization and Evaluation of Optimum pH and TemperatureFatemeh Izadpanah Qeshmi, Mahsa Rahimzadeh, Sedigheh Javadpour, Manijeh PoodatBacterial L-asparaginases are amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukemia. In the present study, a novel strain, Bacillus sp. PG02 was explored for the production of intra-cellular L-asparaginase enzyme. The nitrogen source for L-asparaginase production was L-asparagine. New intracellular L-asparaginase was purified using ion exchange chromatography and … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Nov 2015 · 7,480 accessesView PDFCite