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Research ArticleOpen accessIn the keywordsModification of Artificial Oliogosaccharides in Recombinant Escherichia coli CellsTomohisa Kato, Rie Mitsumori, Kenichi HatanakaArtificial oligosaccharides were modified using recombinant Escherichia coli cells that overexpress sialidase. Based on the principle of the saccharide primer method by using bacterial cells overexpressing enzymes related to oligosaccharide modification. Problem statement: It is very hard to obtain oligosaccharides, because they have complex and diverse structures with different linkage patterns and monosaccharide components. Approach: It had been known that … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,802 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInhibition of Sphingolipid Biosynthesis in Cultured Cells Enhances the Oligosaccharide Production from Exogenous Artifical SubstrateTomohisa Kato, Maria Carmelita Z. Kasuya, Kenichi HatanakaThe effect of fumonisin B1 which is an inhibitor of ceramide synthesis on saccharide elongation of dodecyl β-lactoside (Lac-C12), which is the analogue of lactosyl ceramide (LacCer), in mouse melanoma B16 cells and African green-monkey kidney (Vero) cells was examined. By adding fumonisin B1 in the culture medium used for glycosylation of Lac-C12, mouse melanoma B16 cells synthesized GM3-type analogue … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 6,669 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Identification and Quantification of Marine Fish from Environmental DNA in Sulawesi Waters (Makassar Strait, Flores Sea and Bone Bay), IndonesiaNita Rukminasari, Andi Aliah Hidayani, Wilma Joanna Carolina Moka et al.The emergence of environmental DNA (eDNA) represents a recent methodological breakthrough for evaluating the presence of aquatic vertebrate species. This approach offers a relatively simple method with significant implications for conservation biology. Our study aim was to augment our understanding of marine fish biodiversity in Sulawesi waters. We employed eDNA metabarcoding to investigate fish biodiversity within Sulawesi waters, specifically focusing … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 5 Jul 2024 · 5,005 accessesView PDFCite
Research ArticleOpen accessIn the abstractEarly Detection of Endobacteria in Rhizopus spp.Ratna Noviyanti, Gayuh Rahayu, Rida Oktorida KhastiniRhizopus spp. are commonly used in bio-industrial processes such as manufacturing traditional fermented foods. However, one species, R. microsporus has been reported to contain toxin-producing endobacteria. Therefore, it is essential to evaluate the Rhizopus strains' safety before application as tempe inoculum. The study aimed to detect the presence of endobacteria from seven strains of Rhizopus spp. isolated from Tempe and … …OnLine Journal of Biological Sciences, Vol. 23, Issue 3 · 24 Jun 2023 · 5,331 accessesView PDFCite
Research ArticleOpen accessIn the abstractEstablishment and Application of Rapid Quantitative Detection Technology for Bacillus cereusFeilong Sun, Hanyu Li, Jingxue Qian et al.Infections caused by Bacillus cereus often occur, causing a certain degree of health hazards and economic losses to people around the world. Rapid quantitative detection is of great significance for prevention. This study aims to develop a simple and rapid quantitative detection method for B. cereus, which can be used to monitor the contamination of B. cereus in food samples. … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 1 · 21 Mar 2023 · 5,564 accessesView PDFCite
Research ArticleOpen accessIn the abstractUnderstanding the Epidemiology of Trypanosoma Evansi Infection in Dogs (Canis lupus familiaris) from Urban Areas of ColombiaJeiczon Elim Jaimes-Dueñez, Ángela Patricia Jiménez-Leaño, Daniela Montenegro-Ayala, Maria Esteban-MendozaTrypanosomaevansi is a protozoan parasite that is the causative agent of the animal disease known as "surra", which affects a wide variety of wild and domestic mammals including humans. This study aimed to evaluate the molecular point prevalence and clinic-epidemiological traits of T. evansi infection in dogs from municipalities of the Metropolitan Area of Bucaramanga (MAB), Santander, Colombia. From 2019 … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 2 · 1 Jul 2022 · 5,937 accessesView PDFCite
Research ArticleOpen accessIn the abstractSafety Assessment of Indigenous Probiotic Strain Lactobacillus plantarum Mut-7 Using Sprague Dawley Rats as a ModelAtika Yahdiyani Ikhsani, Emma Riftyan, Rosa Amalia Safitri et al.Lactobacillus plantarum Mut-7 is a probiotic candidate isolated from gatot, traditional fermented cassava from Java, Indonesia. This study aimed to evaluate safety aspects of high dose consumption of L. plantarum Mut-7 (1011 CFU/ml/day) on Sprague Dawley rats for 21 days. Twenty four female rats were randomly divided into 4 groups; initial condition group (P.0), control group (P.1), skim milk group … …American Journal of Pharmacology and Toxicology, Vol. 15, Issue 1 · 30 Apr 2020 · 8,690 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity in Merozoite Surface Protein.1 of Plasmodium falciparum in Highlands: Wamena Papua IndonesiaRosye Hefmi Rechnelty Tanjung, Yulius Sarungu, Meidy Johana Imbiri et al.Malaria is the first killer as well as endemic disease in highland, lowland and coastal areas of Papua. In 2016 there were 160 thousands cases noted and 80% of the cases were caused by Plasmodium falciparum. Total sample for this study were provided by a clinical trial conducted from May to June 2018 in the regional hospital of Puncak Jaya, … …American Journal of Infectious Diseases, Vol. 14, Issue 4 · 11 Jan 2019 · 7,753 accessesView PDFCite
Research ArticleOpen accessIn the abstractSafety Assessment of Indigenous Probiotic Strain Lactobacillus plantarum Dad-13 Isolated from Dadih Using Sprague Dawley Rats as a ModelEndang S Rahayu, Ilzamha H Rusdan, Armita Athennia et al.Lactobacillus plantarum Dad-13 is a probiotic candidate from dadih, a traditional fermented buffalo milk from West Sumatera, Indonesia. This study aimed to evaluate safety aspects of L. plantarum Dad-13 in high dose consumption on Sprague Dawley rats. Two treatment groups were fed with 1011 CFU/mL/day of L. plantarum Dad-13 for 14 and 28 days respectively. Two placebo groups were fed … …American Journal of Pharmacology and Toxicology, Vol. 14, Issue 1 · 12 Nov 2019 · 10,134 accessesView PDFCite
Research ArticleOpen accessIn the abstractMorpho-Physiological and Genetic Responses of Bali Local Rice Cultivars to Drought Stress at Seedling StageMade Pharmawati, Luh Putu WrasiatiRice is the main food source in many countries in the world. One of the major limiting factors for rice growth and productivity is drought stress. In Bali there are several local rice cultivars, two of them are ‘Mansur' and ‘Putih Cempaka'. The cultivar ‘IR 64' is one rice cultivar released by IRRI. The responses of those rice cultivars to … …OnLine Journal of Biological Sciences, Vol. 18, Issue 1 · 29 Mar 2018 · 7,644 accessesView PDFCite