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60 results for “polysaccharide”
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Research ArticleOpen accessEvery word in the titleOptimization of Chelating Process of Polysaccharides of Lyophyllum decastes with Zinc and Its Antioxidant ActivityBing Xu, Yi Dai, Jing Hong, Yiyong ChenIn this study, polysaccharides of Lyophyllum decastes (LDS) were used as raw material. The chelating rate was set as the index, the effect of chelating time, mass ratio and initial mass concentration on chelating rate was investigated. Response surface method was used to optimize the preparation technology of LDS and Zinc (Ⅱ) chelate (LDS-Zn) based on single factor experiments. LDS-Zn … …Current Research in Bioinformatics, Vol. 9, Issue 1 · 26 Aug 2020 · 11,761 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInhibitory Effect of Crude Walnut Leaf Polysaccharides on Oxidative Damage of Proteins and Proliferation of SGC-7901 CellsShuqing Yang, Zhike Xie, Shuyan Yu et al.In this paper, we studied the inhibitory effect of crude Walnut Leaf Polysaccharides (WLP) on free radical-induced oxidative damage of proteins and anti-proliferation activity on human Gastric Cancer cells (SGC-7901). The oxidative degradation of Bovine Serum Albumin (BSA), mouse brain proteins (MBP) and Mouse Liver Proteins (MLP) induced by hydroxyl radical and alcoxyl radical was analyzed by Sodium Dodecyl Sulfate-Polyacrylamide … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 26 Apr 2020 · 7,057 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOptimization of Ultrasonic/Microwave Assisted Extraction (UMAE) and Rheological Properties of Polysaccharides from Auricularia polytrichaZe Zhang, Xiao Ding, Ziyi Xu et al.Polysaccharides from Auricularia polytricha (APPS) were extracted using Ultrasonic/Microwave Assisted Extraction (UMAE) technology. UMAE conditions of APPS were optimized with Response Surface Methodology (RSM). Rheological properties of APPS were investigated. The results suggested that the optimal UMAE conditions for APPS were ratio of water to raw material 22 (mL/g), extraction time 15.3 min and microwave power 59W. APPS solution exhibited … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 14 Mar 2020 · 7,420 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOptimization for the Extraction of Polysaccharide from Walnut (Juglans regia L.) Leaves: Antioxidant Activities in VitroShuqing Yang, Haifang Xiao, Shuyan Yu et al.Cellulase-assisted extraction of Walnut Leaf Polysaccharide (WLP) was studied. Single factor test and response surface design were employed to optimize the technological conditions. The consequences indicated that the highest WLP yield of 5.21% was achieved with raw material ratio of 50 mL/g, cellulase dose of 41488.52 U/g, enzymolysis pH of 5.88, enzymolysis time of 29.74 min and enzymolysis temperature of … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 23 Oct 2019 · 7,450 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Deproteinization, Antioxidant Acticities and Inhibitory Effect on α-Amylase of Polysaccharides from Corn SilkYaping Li, Songbiao Shi, Xiudong Yang, Hongli ZhouAn approach of deproteinization of polysaccharides from corn silk (CSPs) was established to improve the purity and activities of CSPs by investigating Sevag method, trichloroacetic acid (TCA) method and HCl method. Deproteinization rate, polysaccharide retention rate, antioxidant activities and inhibition α-amylase effects were used as indicative mark to select suitable deproteinization method of CSPs. The crude CSPs was extracted by … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 23 May 2019 · 9,294 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInhibition Effect of Walnut Leaf Polysaccharide on HepG2 Cells in vitroShuqing Yang, Haifang Xiao, Shuyan Yu et al.In this study, the effect of Walnut Leaf Polysaccharides (WLP) on human liver tumor HepG2 cells was investigated. The results showed that WLP had dose-dependent and time-dependent inhibitory effects on HepG2 cell viability. Typical apoptosis characteristics were observed in WLP-treated HepG2 cells through 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolyl-carbocyanine iodide (DAPI) staining and Acridine Orange (AO)/Ethidium Bromide (EB) dual staining. Meanwhile, significant decrease in Mitochondrial … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 29 Oct 2019 · 7,313 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleImmunological Evidence for Peptide-Carbohydrate Mimicry with a Group A Streptococcus Polysaccharide-Mimetic PeptideSilvia Borrelli, Rehana B. Hossany, Susan Findlay, B. Mario PintoThe immunogenicity of a peptide-protein conjugate developed by linking a peptide mimic DRPVPY of the Group A Streptococcus cell-wall polysaccharide (GAS-CWPS), to tetanus toxoid (TT) was examined. BALB/c mice were immunized three times subcutaneously following homologous or heterologous prime/boost strategies at 4- or 6- week intervals in two different experiments. DRPVPY-TT, CWPS-TT, heat-killed, pepsin-treated GAS bacteria (with exposed polysaccharide) and … …American Journal of Immunology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,956 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleFabrication of Polysaccharides from a Fermented Tea and Their Antioxidant and Hypoglycemic ViabilityJun Li, Lingxian Tong, Bing Li et al.The hypoglycemic effects of tea have been extensively studied, with fermented tea demonstrating notable efficacy owing to its distinct processing methods. This study involved the extraction of tea polysaccharides from Wuniuzao Black Tea (WTP) using a water extraction and ethanol precipitation method. WTP-1 and WTP-2 were obtained by separation and purification using DEAE-52 cellulose columns. Antioxidant activity was evaluated utilizing … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 2 · 30 Jul 2026 · 655 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleImmunomodulatory and Antioxidant Activities of Selenium-Enriched Polysaccharides from Epimedium and Isatis IndigoticaRui Zhang, Jin Li, Jiajia ZhouThis study aims to investigate the effects of selenium modification on polysaccharides derived from two traditional Chinese medicinal herbs-Epimedium (Yinyanghuo) and Isatis root (Banlangen)-in enhancing immune function and antioxidant activity. The purpose is to explore the relationship between structure and function, providing a theoretical basis for the development of efficient plant-based functional antioxidants. Polysaccharides were extracted using an optimized enzymatic … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 3 Feb 2026 · 1,933 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleSeparation, Purification and Anti-aging Activity of Tobacco PolysaccharidesMengyue Huang, Jingwu Song, Shuaishuai Chang et al.To separate tobacco polysaccharides and investigate their antioxidant and anti-aging properties, crude tobacco polysaccharides were extracted from raw tobacco using hot water, followed by purification through DEAE-52 cellulose column chromatography. The molecular weight and monosaccharide composition of the isolated polysaccharides were analyzed using HPGPC and HPLC. The antioxidant activities of these tobacco polysaccharides were tested using DPPH, ABTS, hydroxyl radicals … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 15 Jan 2025 · 5,103 accessesView PDFCite