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Research ArticleOpen accessIn the abstractMining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3Miaomiao Sheng, Huake Jia, Xiaomai Tao et al.Objective of this paper is to excavate the siderophore synthesis gene from Brevibacillus brevis GZDF3 and verify its type and antibacterial effects. The method is using genome mining technology to analyze the siderophore synthesis genes and the phylogenetic tree of each synthesis gene was constructed separately. Iron free medium was utilized to induce the synthesis of siderophore and CAS liquid … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 3 · 28 Aug 2018 · 10,606 accessesView PDFCite
Research ArticleOpen accessIn the abstractQuercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine NeutrophilsSuphakit Srikok, Sukij Nambut, Kanruethai Wongsawan, Phongsakorn ChuammitriThis study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or 50 µM of Quercetin (QH) or Cytochalasin B (CytB), as phagocytosis inhibitor, prior to monitoring phagocytosis of Escherichia coli by flow cytometry and microscopic examination. Additionally, the expressions … …American Journal of Animal and Veterinary Sciences, Vol. 12, Issue 2 · 2 Aug 2017 · 8,120 accessesView PDFCite
Review ArticleOpen accessIn the abstract"Ebola Ça Suffit!" is not enough to Prove Efficacy of an Ebola VaccineDavid R CroweA recently published Ebola vaccine trial claimed an almost perfect result with no cases of Ebola after the vaccine had time to take effect - a vaccine efficacy of 100%. This article is a detailed review of the information published about the trial and, particularly, this claim. The trial design was sub-optimal because randomization was only partial (and one treatment … …American Journal of Immunology, Vol. 13, Issue 3 · 8 Jul 2017 · 17,825 accessesView PDFCite
Research ArticleOpen accessIn the abstractScreening of Yeast Strains for Pectinolytic Activity: Effects of Different Carbon and Nitrogen Sources in Submerged FermentationsMustafa Oskay, Hüsniye Tansel YalçinTwenty yeast strains were screened for pectinolytic activity, among the strains, only nine were positive for pectinase production. The best strain was Kluyveromyces marxianus NRRL-Y-1109 which gave high quantities of pectinase activity, by submerged fermentation. Different parameters such as incubation time, pH (3.5-7), temperature (20-45°C), nitrogen and carbon source, were optimized. The optimal incubation time, temperature and pH for pectinase … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 22 Jun 2015 · 7,812 accessesView PDFCite
Research ArticleOpen accessIn the abstractOPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORMNayera A.M. Abdelwahed, Enas N. Danial, Noura El-Ahmady El-Naggar, Asem A. MohamedOptimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL-1 were 30°C at pH 8.5 and incubation time 96 h in free cell cultures using starch 20 g L-1 as carbon source and yeast extract 5 g L-1 … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Dec 2013 · 8,125 accessesView PDFCite
Research ArticleOpen accessIn the abstractUse of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid DamageClara Gonzalez-Marin, Michael Eduard Kjelland, Rosa Roy et al.Cryopreserved sperm samples from Holstein bulls (n = 20) were examined for bacterial presence and Sperm DNA Fragmentation (SDF) dynamics. SDF was assessed after thawing (T0) and at 4, 24 and 48 h of incubation (37°C) and the rate of SDF (r-SDF), as an estimator of the DNA degradation over time, was calculated. Two groups of bulls were identified based … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 4 · 29 Dec 2012 · 7,430 accessesView PDFCite
Research ArticleOpen accessIn the abstractAerobic Biodegradation of Per-Treated Methyl Tert-Butyl Ether By Ozonation in an Up-Flow-Fixed-Bed ReactorM. Sadeghi, M. Arbabi, A. Nikpey, G. MardaniProblem Statement: MTBE is a common pollution of environmental and has become an issue of considerable concern in recent years. It is not readily amenable to remove MTBE by conventional techniques in water treatment. In the present study, the feasibility of the continuous aerobic biodegradation of MTBE, was evaluated in an Up- Flow Fixed Bed Reactor (UFBR). Approach: The UFBR … …American Journal of Environmental Sciences, Vol. 5, Issue 3 · 30 Jun 2009 · 6,620 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of some by-Products using In situ and In vitro Gas Production TechniquesBesharati Maghsoud, Taghizadeh Akbar, Janmohammadi Hossein, Moghadam Gholam AliFood by-products in Iran are produced in high levels. In this study, in situ and in vitro gas production techniques were used to describe nutritive value of apple pomace, tomato pomace and noodle waste. For this purpose two ruminal fistulated sheep were used. Nylon bags which were approximately (6×12 cm) containing 5 g samples (2 mm screen) were incubated in … …American Journal of Animal and Veterinary Sciences, Vol. 3, Issue 1 · 31 Mar 2008 · 6,945 accessesView PDFCite
Research ArticleOpen accessIn the abstractHigh Concentrations of Glucose can Activate or Inhibit Human Erythrocyte Aminolevulinate Dehydratase in vitro Depending Exposure TimeJ. C. Soares, D. Gabriel, V. Folmer et al.Hyperglycemia can cause oxidative stress and inactivate susceptible enzymes. In the present investigation we examined whether short-term incubations (24 or 48 h) with high concentrations of glucose (10-200 mmol L-1) inhibit ALA-D from human erythrocytes. Incubations of erythrocytes for 24 h with glucose (10 up to 40 mmol L-1) resulted in an increase of δ-ALA-D activity (P<0,001). Incubations of erythrocytes … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,686 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnzymatic Hydrolysis of Pretreated Kenaf using a Recombinant Xylanase: Effects of Reaction Conditions for Optimum Hemicellulose HydrolysisNur Izyan Wan Azelee, Jamaliah Md Jahim, Ahmad Fauzi Ismail et al.An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstrated. Experiments were performed primarily to investigate the effect of several critical reaction conditions towards the enzymatic hydrolysis of the pre-treated kenaf. The effects of … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 2 · 28 Apr 2016 · 7,816 accessesView PDFCite